Electron microscope study on the replication of AAV-1 in herpes-infected cells

Spatial and Temporal Organization of Adeno-Associated Virus DNA Replication in Live Cells

“…The molecular mechanisms of AAV DNA replication are well investigated (2), but relatively few studies report on the spatial organization of AAV DNA and proteins within the host cell nucleus (13,16,52,54). Hunter and Samulski (16) showed that in Ad-infected cells, all four Rep proteins colocalized in distinct nuclear foci.…”

“…While the molecular mechanisms of AAV DNA replication are well investigated (2), relatively few studies report on the spatial organization of AAV DNA and proteins within the nucleus of the infected cell (13,16,52,54). Using immunofluorescence microscopy, Hunter and Samulski (16) demonstrated that all four Rep proteins occupy the same intranuclear compartments and that Rep and capsid proteins colocalized.…”

Isolation and preliminary characterisation of a small nuclear inclusion virus infecting the diatom Chaetoceros cf. gracilis

“…3 suggests that formation of the paracrystalline array may be connected to fragmentation of the nucleolus. AlLami et al (1969) and Henry et al (1972) showed that large masses of empty capsids of 2 parvoviruses were closely associated with, and sometimes replaced, fragmented nucleoli, suggesting the assembly and possible synthesis of viral capsids by the nucleolus. The fact that individual viral particles were not observed within infected C. cf.…”

Helper Functions Required for Wild Type and Recombinant Adeno- Associated Virus Growth

“…However, the first reports indicated that HSV-1 was only able to induce AAV protein synthesis without detectable production of AAV particles [Atchison, 1970;Blacklow et al, 1970]. This notion was rapidly altered by the observation by electron micsoscopy of AAV capsids in the nucleus of cells coinfected with wt AAV-1 and HSV-1 [Henry et al, 1972]. Further studies conducted with wt AAV-2 confirmed this finding and also indicated that co-infection with HSV-1 or -2 resulted in AAV titers similar to those observed in the presence of Ad [Buller et al, 1981;Handa and Carter, 1979].…”