Electron dense alteration of kidney basement membranes

Chemical analysis of the glomeruli from the kidney of diabetics and subjects with choronic glomerulonephritis.

“…Westberg and Michael (1973b) reported that the content of hydroxyproline and hydroxylysine appeared to be reduced in chronic glomerulonephritis and idiopathic membranoproliferative glomerulonephritis, and the ratio of hydroxyproline to hydroxylysine in these groups, 3.3, was slightly lower than that in the control group (4.04). On the other hand, Galle and Machieu (1975) reported that the content of hydroxyproline and hydroxylysine was almost same in both of the control and glomerulonephritis groups. In our study, the content of hydroxyproline and O-hydroxylysylglycosides in the glomeruli of the glomerulonephritis groups did not differ significantly from the counterpart values in the control.…”

“…The chemical composition of the basement membrane isolated from the human kidney of glomerulonephritis cases was studied by Westberg and Michael (1973b), and Galle and Machieu (1975). Westberg and Michael (1973b) reported that the content of hydroxyproline and hydroxylysine appeared to be reduced in chronic glomerulonephritis and idiopathic membranoproliferative glomerulonephritis, and the ratio of hydroxyproline to hydroxylysine in these groups, 3.3, was slightly lower than that in the control group (4.04).…”

“…Chemical analysis of the glomerular basement membrane has been carried out to obtain information in regard to its structural alteration in pathological conditions such as diabetes mellitus and glomerulonephritis (Beisswenger and Spiro 1973;Westberg and Michael 1973a, b;Kefalides 1974;Sato et al 1975;Galle and Machieu 1975). There is, however, no general agreement concerning the changes in the chemical compositions of the glomerular basement membrane in these diseases.…”

Effect of hemodynamic factors in renal glomerulus. I. Alterations of glomerular basement membrane in the experimental congestive kidney.

“…Galle (11) first recognized the unique disorder of the glomerular basement membrane structure in an ultrastructural analysis of a form of proliferative glomerulonephritis. In this study an extremely electron dense "deposit" was noted within the substance of the glomerular basement membrane proper.…”

“…Electron micrographs of the syndrome showed a marked irregular thickening of the glomerular basement membrane with extensive deposition of dark fibers surrounded by narrow clear zones of rarefaction (1) . In the membranoproliferative glomerulonephritis, which is most often clinically manifested by heavy proteinuria accompanied by recurring hematuria, and is a typical dense deposit desease, an electron-dense transformation was recognized in biopsy materials (11). In diabetic nephropathy, diffuse thickening was observed (25, 26, 28).…”