Chemical analysis of the glomeruli from the kidney of diabetics and subjects with choronic glomerulonephritis.

Studies on amino acid composition of renal glomeruli from the diabetics.

“…For the preparation of the glomeruli from the kidneys fixed in formaldehyde, cortical slices (about 2 g) were disrupted on 115-mesh stainless steel sieve of 7.5 cm in diameter and filtered through a 200-mesh sieve positioned under 80-mesh sieve. The glomerular fraction was obtained on the 200-mesh sieve, from which about 1,500 pieces of the glomeruli were picked up and collected at random under a stereomicroscope (Sato et al 1980). The glomeruli of nonfixed kidneys from 3 autopsy subjects were prepared according to the method of Krakower and Greenspon (Krakower and Greenspon 1951).…”