Electron- and immunoelectron-microscopic investigation on the rabbit haemorrhagic disease virus

Alexandrov, M.; Peshev, R.; Bozhkov, S; Yanchev, I.; Doumanova, L.

doi:10.1016/0147-9571(93)90057-c

Cited by 8 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of two types of intact virions with high or low density inside was consistent with our previous experiments (Zheng et al, 2001). The existence of CLP in purified RHDV was also reported by negative staining (Alexandrov et al, 1993), but this is the first time to observe two types of CLPs with high or low density inside.…”

Section: Resultssupporting

confidence: 80%

See 1 more Smart Citation

Cryo-electron microscopy reconstructions of two types of wild rabbit hemorrhagic disease viruses characterized the structural features of Lagovirus

Tian

Zhai

et al. 2010

Protein Cell

View full text Add to dashboard Cite

Rabbit hemorrhagic disease was described in China in 1984 and can cause hemorrhagic necrosis of the liver within two or three days after infection. The etiological agent, rabbit hemorrhagic disease virus (RHDV), belongs to the Lagovirus genus in the Caliciviridae family. Compared to other calicivirus, such as rNV and SMSV, the structure of Lagovirus members is not well characterized. In this report, structures of two types of wild RHDV particles, the intact virion and the core-like particle (CLP), were reconstructed by cryo-electron microscopy at 11 Å and 17 Å, respectively. This is the first time the 3D structure of wild caliciviruses CLP has been provided, and the 3D structure of intact RHDV virion is the highest resolution structure in Lagovirus. Comparison of the intact virion and CLP structures clearly indicated that CLP was produced from the intact virion with the protrusion dissociated. In contrast with the crystal structures of recombinant Norovirus and San Miguel sea lion virus, the capsomers of RHDV virion exhibited unique structural features and assembly modes. Both P1 and P2 subdomains have interactions inside the AB capsomer, while only P2 subdomains have interaction inside CC capsomer. The pseudo atomic models of RHDV capsomers were constructed by homology modeling and density map fitting, and the rotation of RHDV VP60 P domain with respect to its S domain, compared with SMSV, was observed. Collectively, our cryo-electron microscopic studies of RHDV provide close insight into the structure of Lagovirus, which is important for functional analysis and better vaccine development in the future.

show abstract

Section: Resultssupporting

confidence: 80%

“…This particle had a smooth surface with 25-29 nm diameter (Alexandrov et al, 1993;Granzow et al, 1996). The molecular weight of CLP subunit was about 28-30 kDa corresponding to the N-terminal part of VP60.…”

Section: Introductionmentioning

confidence: 99%

Cryo-electron microscopy reconstructions of two types of wild rabbit hemorrhagic disease viruses characterized the structural features of Lagovirus

Tian

Zhai

et al. 2010

Protein Cell

View full text Add to dashboard Cite

show abstract

“…These particles are found in large amounts in the liver and spleen [54] and present unique characteristics when compared to RHDV particles: a smooth surface due to the lack of the cup-shaped depressions; a smaller diameter of 25-29 nm; a molecular weight of 28-30 kDa indicating that CLP correspond to the N-terminus (the buried shell domain) of the capsid; no haemagglutinating activity, most likely as the result of the absence of the C-terminus, but presenting reactivity with sera from RHDV convalescent rabbits and monoclonal antibodies directed towards the N-terminal part of the RHDV capsid [35,54-56]. CLP seem to be associated with the appearance of specific anti-RHDV IgM [54].…”

Section: Aetiological Agentmentioning

confidence: 99%

Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review

Abrantes

Loo

Pendu

et al. 2012

Vet Res

338

452

View full text Add to dashboard Cite

Rabbit haemorrhagic disease virus (RHDV) is a calicivirus of the genus Lagovirus that causes rabbit haemorrhagic disease (RHD) in adult European rabbits (Oryctolagus cuniculus). First described in China in 1984, the virus rapidly spread worldwide and is nowadays considered as endemic in several countries. In Australia and New Zealand where rabbits are pests, RHDV was purposely introduced for rabbit biocontrol. Factors that may have precipitated RHD emergence remain unclear, but non-pathogenic strains seem to pre-date the appearance of the pathogenic strains suggesting a key role for the comprehension of the virus origins. All pathogenic strains are classified within one single serotype, but two subtypes are recognised, RHDV and RHDVa. RHD causes high mortality in both domestic and wild adult animals, with individuals succumbing between 48-72 h post-infection. No other species has been reported to be fatally susceptible to RHD. The disease is characterised by acute necrotising hepatitis, but haemorrhages may also be found in other organs, in particular the lungs, heart, and kidneys due to disseminated intravascular coagulation. Resistance to the disease might be explained in part by genetically determined absence or weak expression of attachment factors, but humoral immunity is also important. Disease control in rabbitries relies mainly on vaccination and biosecurity measures. Such measures are difficult to be implemented in wild populations. More recent research has indicated that RHDV might be used as a molecular tool for therapeutic applications. Although the study of RHDV and RHD has been hampered by the lack of an appropriate cell culture system for the virus, several aspects of the replication, epizootology, epidemiology and evolution have been disclosed. This review provides a broad coverage and description of the current knowledge on the disease and the virus.

show abstract

Demonstration of rabbit haemorrhagic disease virus antigen by Staphylococcus protein A coagglutination test

Peshev¹,

Alexandrov

Ivanov³

et al. 1996

Journal of Virological Methods

View full text Add to dashboard Cite

Electron- and immunoelectron-microscopic investigation on the rabbit haemorrhagic disease virus

Cited by 8 publications

References 10 publications

Cryo-electron microscopy reconstructions of two types of wild rabbit hemorrhagic disease viruses characterized the structural features of Lagovirus

Cryo-electron microscopy reconstructions of two types of wild rabbit hemorrhagic disease viruses characterized the structural features of Lagovirus

Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review

Demonstration of rabbit haemorrhagic disease virus antigen by Staphylococcus protein A coagglutination test

Contact Info

Product

Resources

About