Demonstration of rabbit haemorrhagic disease virus antigen by Staphylococcus protein A coagglutination test

Peshev, R.; Alexandrov, M.; Ivanov, Y.; Gogov, Yordan

doi:10.1016/0166-0934(95)01914-6

Cited by 2 publications

(4 citation statements)

References 12 publications

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“…The test revealed high specificity and sensitivity and better performance than the HA [180]. However, and while the Sp A COAT does not require any special equipment and provides fast results [180], it does require rabbits to be infected with RHDV in order to obtain anti-RHDV antibodies raising concerns in terms of animal welfare and it does not allow genotype or variant identification. A Luminex xMAP assay has also been developed, allowing a specific detection of anti-RHDV antibodies [179].…”

Section: Staphylococcus Protein a Coagglutination Testmentioning

confidence: 99%

“…The Staphylococcus protein A coagglutination test (Sp A COAT) is a simple, specific and sensitive test that was developed as a rapid test for the detection of RHDV [ 180 ]. The test was adapted from a slide-agglutination method for pneumococci typing based on the ability of the Fc fragments of anti-pneumococci IgG to bind to the Staphylococcus aureus protein-A (Sp A) located in the cell wall [ 181 ].…”

Section: Staphylococcus Protein a Coagglutination Testmentioning

confidence: 99%

“…This binding directs the Fab structures outwards and coagglutination occurs when a suspension of homologous pneumococci antigens is added [ 181 ]. Liver, spleen, kidney and lung samples were successfully used for RHDV antigen detection by Sp A COAT with Staphylococcus aureus sensitized with rabbit anti-RHDV antibodies ( Figure 7 ) [ 180 ]. The test revealed high specificity and sensitivity and better performance than the HA [ 180 ].…”

Section: Staphylococcus Protein a Coagglutination Testmentioning

confidence: 99%

“…Liver, spleen, kidney and lung samples were successfully used for RHDV antigen detection by Sp A COAT with Staphylococcus aureus sensitized with rabbit anti-RHDV antibodies ( Figure 7 ) [ 180 ]. The test revealed high specificity and sensitivity and better performance than the HA [ 180 ]. However, and while the Sp A COAT does not require any special equipment and provides fast results [ 180 ], it does require rabbits to be infected with RHDV in order to obtain anti-RHDV antibodies raising concerns in terms of animal welfare and it does not allow genotype or variant identification.…”

Section: Staphylococcus Protein a Coagglutination Testmentioning

confidence: 99%

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A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV)

Abrantes

Lopes

2021

Microorganisms

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Since the early 1980s, the European rabbit (Oryctolagus cuniculus) has been threatened by the rabbit hemorrhagic disease (RHD). The disease is caused by a lagovirus of the family Caliciviridae, the rabbit hemorrhagic disease virus (RHDV). The need for detection, identification and further characterization of RHDV led to the development of several diagnostic tests. Owing to the lack of an appropriate cell culture system for in vitro propagation of the virus, much of the methods involved in these tests contributed to our current knowledge on RHD and RHDV and to the development of vaccines to contain the disease. Here, we provide a comprehensive review of the RHDV diagnostic tests used since the first RHD outbreak and that include molecular, histological and serological techniques, ranging from simpler tests initially used, such as the hemagglutination test, to the more recent and sophisticated high-throughput sequencing, along with an overview of their potential and their limitations.

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Section: Staphylococcus Protein a Coagglutination Testmentioning

confidence: 99%

Section: Staphylococcus Protein a Coagglutination Testmentioning

confidence: 99%

Section: Staphylococcus Protein a Coagglutination Testmentioning

confidence: 99%

Section: Staphylococcus Protein a Coagglutination Testmentioning

confidence: 99%

See 2 more Smart Citations

A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV)

Abrantes

Lopes

2021

Microorganisms

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Coagglutination for viral DNA preparation of canine parvovirus for molecular diagnosis

Ribeiro

Araújo

2009

Journal of Virological Methods

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The use of PCR for molecular diagnosis is accepted as the standard method for detecting nucleic acids from numerous major infectious agents using diverse sampling techniques. Although PCR is an essential tool in the research laboratory; the success of the method depends on a sample free of inhibitors that was obtained preferably by a simple and fast extraction method. The coagglutination test (COA test) is based on the propriety of protein A, from Staphylococcus aureus, which can bind specifically to the Fc portion of immunoglobulin G (IgG) in various mammals and to several IgG subclasses in mice. In this study, the COA method capacity of generating inhibitor-free DNA sample was tested. Ten fecal samples positive for canine parvovirus were subjected to nucleic acid extraction using COA method and a commercial kit (Illustra GFX Genomic Blood DNA Purification Kit/G.E. Healthcare); and the samples' viral DNA content were compared using real-time PCR. The COA procedure allowed the extraction of larger amount of viral DNA from feces than the commercial kit. This method was shown to be simple and effective for DNA extraction, concentrating viral particles dispersed in the biological samples.

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Demonstration of rabbit haemorrhagic disease virus antigen by Staphylococcus protein A coagglutination test

Cited by 2 publications

References 12 publications

A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV)

A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV)

Coagglutination for viral DNA preparation of canine parvovirus for molecular diagnosis

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