Electromagnetic field effects on Artemia hatching and chromatin state

Shckorbatov, Y. G.; Rudneva, I. I.; Pasiuga, V. N.; Grabina, V.A.; Kolchigin, N. N.; Ivanchenko, D.D.; Kazanskiy, O.V.; Shayda, Valentin G.; Dumin, Oleksandr

doi:10.2478/s11535-010-0063-8

Cited by 8 publications

(5 citation statements)

References 17 publications

(22 reference statements)

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“…We investigated different stirring speeds of 0, 80, and 400 rpm with magnetic stirring. To compare magnetic stirring with mechanical stirring, we also conducted an experiment using a home-made overhead stirrer, which minimized the impact of magnetic field that can be generated by magnetic stirring, and potentially improve cyst hydration (Yuriy et al 2010, Sagdilek et al 2013). Results are shown in Figure 3 .…”

Section: Resultsmentioning

confidence: 99%

“…05 ), the CHN of 80 rpm by magnetic stirring after 16 h increased markedly faster (slope = 5.78, blue line, Figure 3 ) to its maximum than that of 80 rpm by overhead stirring (slope = 2.12, red line, Figure 3 ), indicating that magnetic stirring may be conducive to cyst hydration. Yuriy et al suggested that magnetic field-induced chromatin granule formation in Artemia nauplia could promote dormant cyst activation (Yuriy et al 2010). The effect of magnetic field on cyst hydration is beyond the scope of this study but worthy of further investigation.…”

Section: Resultsmentioning

confidence: 99%

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Which waters hydrate best? A study using brine-shrimp cysts (Artemia franciscana)

Pollack

2020

Preprint

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Hydration plays a particularly important role in health maintenance and general well-being. A wide assortment of drinking waters are currently available on the market. However, their ability to hydrate may vary. For studying hydration, a useful organism may be the cysts of brine shrimp. Those cysts may remain dehydrated and functionless for years, but regain function once hydrated. In this study, we first determined the optimal factors for assessing hydration in the brine-shrimp model, including aeration method and flow rate, salinity, and temperature. Various kinds of water, including tap water, bottled water, and water containing health-promoting agents, were tested by using this new method to evaluate their ability to hydrate. Tap water showed weak hydration, while some bottled waters (e.g., Kirkland Signature Purified Water) hydrated more effectively. Mineral water, such as Fiji water, was found to be a desirable option to maintain adequate and lasting hydration.Graphic abstract

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Which waters hydrate best? A study using brine-shrimp cysts (Artemia franciscana)

Pollack

2020

Preprint

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“…Виявлений нами набряк саркоплазми, який викликає розшарування міофібрил вже через чотири тижні після початку моделювання експериментального стрептозотоцинового цукрового діабету поряд зі значним зменшенням осміофільності нуклеоплазми у м'язових волокнах проміжного типу можна розглядати як прояв функціонального виснаження ядра (Shckorbatov et al, 2010).…”

Section: обговоренняunclassified

Left ventricular diastolic function in patients with obstructive sleep apnoea syndrome and obesity

Luchynskyi

Luchinskyi

Shcherba

et al. 2017

Regul. Mech. Biosyst.

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Obstructive sleep apnea syndrome (OSAS) is a chronic disease characterized by recurrent episodes of complete or partial obstruction of the upper airways during sleep and results in sleep fragmentation, night hypoxemia, and daytime sleepiness. The aim of this study was to investigate the state of left ventricular diastolic function in patients with first diagnosed COAS and obesity and to define the determinants of diastolic dysfunction in this category of patients. There were 46 patients (33 men and 13 women) with OSAS and obesity and 52 patients with obesity without OSAS enrolled in the study. The control group included 22 practically healthy individuals. Each patient underwent assessment of body mass index (BMI), 24-hour ambulatory blood pressure monitoring, cardiorespiratory monitoring, transthoracic echocardiography. We found that patients with OSAS and obesity had significant impairment of diastolic function in comparison with patients with obesity without OSAS, which manifested itself in decreasing of E/A ratio, increasing deceleration time and increasing isovolumic relaxation time. Increasing body mass index and increasing level of hypoxia lead to impairment of diastolic dysfunction. Diastolic dysfunction was diagnosed in 4 obese patients without OSAS and in 17 patients with OSAS and obesity. All patients with diastolic dysfunction had second or third stage obesity and severe OSAS. Left ventricular hypertrophy was diagnosed in 9 patients with OSAS and obesity. Patients with concentric hypertrophy had significantly lower E/A in comparison with patients without hypertrophy or with concentric remodeling. We found that the deceleration time has positive correlation with body mass index and desaturation index and negative correlation with min SaO2. Isovolumic relaxation time correlated only with desaturation index. According to the results of univariable regression analysis, five variables were allocated: body mass index, age, desaturation index, index apnea-hypopnea and left ventricular mass index. The multivariate analyses, after adjustment for age and sex and stepwise regression, showed that only the desaturation index was an independent predictor of left ventricular diastolic dysfunction in patients with OSAS and obesity. Obstructive sleep apnea syndrome combined with obesity leads to a deterioration of the diastolic function of the left ventricle even in patients without cardio-vascular diseases. The state of the diastolic function of the left ventricule worsens with an increase in the severity of hypoxic disorders in the syndrome of obstructive sleep apnea and with the degree of obesity. In multivariate regression analysis, the desaturation index was a predictor of left ventricular diastolic dysfunction in patients with obstructive sleep apnea syndrome and obesity.

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“…The simple method for determination of the portion of heterochromatin by counting the number of heterochromatin granules in a probe of cells and determination of average number per cell was proposed for buccal epithelium cells -cells of cheek mucosa [109]. In a series of works it was demonstrated that the low energy EMF (10 -100 µW/cm 2 ) and static magnetic field (25 mT) induce chromatin condensation (heterochromatinization) [38,47,[110][111][112][113][114]. It is supposed that this phenomenon reflects the process of regulation of genetic activity at cytological level, because heterochromatinization is connected with decrease of functional activity of chromatin [115,116].…”

Section: The Action Of Emf On Intracellular Regulatory Systemsmentioning

confidence: 99%

Electromagnetic field effects on Artemia hatching and chromatin state

Cited by 8 publications

References 17 publications

Which waters hydrate best? A study using brine-shrimp cysts (Artemia franciscana)

Which waters hydrate best? A study using brine-shrimp cysts (Artemia franciscana)

Left ventricular diastolic function in patients with obstructive sleep apnoea syndrome and obesity

The Main Approaches of Studying the Mechanisms of Action of Artificial Electromagnetic Fields on Cell

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