Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

Abstract

We present an electrokinetic label-free biomolecular screening chip (Glass/PDMS) to screen up to 10 samples simultaneously using surface plasmon resonance imaging (iSPR). This approach reduces the duration of an experiment when compared to conventional experimental methods. This new device offers a high degree of parallelization not only for analyte samples, but also for multiplex analyte interactions where up to 90 ligands are immobilized on the sensing surface. The proof of concept has been demonstrated with well-known biomolecular interactant pairs. The new chip can be used for high throughput screening applications and kinetics parameter extraction, simultaneously, of interactant-protein complex formation.

Microfluidic Chemical Analysis Systems

“…Use of liquid chromatography has been more limited owing to the difficulties involved in high-pressure applications at the microscale, but some work has been conducted (48). Recently, use of surface plasmon resonance (SPR) imaging techniques in microfluidic devices has been increasing; SPR is attractive because it requires no labeling (49). In addition to separation, concentration of species is also possible, usually through the use of nanochannels to generate exclusion regions or extended space charge regions (50).…”

Improved polarization contrast method for surface plasmon resonance imaging sensors by inert background gold film extinction

“…The gold film array in Fig. 1 (a) is one of the most popular structures, which is constructed by depositing an array of thin gold film on the surface of a bare prism [14,15]. The gold film arrays work as sensing spots while the bare prism parts around them form the "background".…”

CMOS Active Column Sensor for biodetection applications based on Surface Plasmon Resonance

“…usually involves the analysis and manipulation of biological agents such as DNA, proteins, antibodies, enzymes, virus, etc. A particular phenomenon that can be applied to biodetection is Surface Plasmon Resonance, which has been used extensively in biosensor [2][3][4]. An SPR array usually consists of a functionalized SPR structure, excitation light source, coupling optics and photodetection stage.…”