Electrohydrodynamically enhanced drying droplets for concentration of Salmonella bacteria prior to their detections using antibody-functionalized SERS-reporter submicron beads

Su, Shih-Rong; Chen, Yuan-Yu; Li, Kuan-Ying; Fang, Yu-Cheng; Wang, Chih‐Hsien; Yang, Chiou-Ying; Chau, Lai-Kwan; Wang, Shau‐Chun

doi:10.1016/j.snb.2018.12.048

Cited by 14 publications

(19 citation statements)

References 31 publications

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“…(a) A colorimetric biosensor based on gold nanoparticles aggregation (Ma et al., 2017). (b) A label‐based surface‐enhanced Raman scattering biosensor using nanoaggregate‐embedded beads as labels (Su et al., 2019). (c) Direct and reverse fluorescent biosensing strategies based on immunomagnetic beads and quantum dots (Yin et al., 2016).…”

Section: Biosensors With Different Transduces For Detection Of Salmonmentioning

confidence: 99%

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Biosensors for rapid detection of Salmonella in food: A review

Shen

2020

Comp Rev Food Sci Food Safe

136

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Salmonella is one of the main causes of foodborne infectious diseases, posing a serious threat to public health. It can enter the food supply chain at various stages of production, processing, distribution, and marketing. High prevalence of Salmonella necessitates efficient and effective approaches for its identification, detection, and monitoring at an early stage. Because conventional methods based on plate counting and real‐time polymerase chain reaction are time‐consuming and laborious, novel rapid detection methods are urgently needed for in‐field and on‐line applications. Biosensors provide many advantages over conventional laboratory assays in terms of sensitivity, specificity, and accuracy, and show superiority in rapid response and potential portability. They are now recognized as promising alternative tools and one of the most on‐site applicable and end user–accessible methods for rapid detection. In recent years, we have witnessed a flourishing of studies in the development of robust and elaborate biosensors for detection of Salmonella in food. This review aims to provide a comprehensive overview on Salmonella biosensors by highlighting different signal‐transducing mechanisms (optical, electrochemical, piezoelectric, etc.) and critically analyzing its recent trends, particularly in combination with nanomaterials, microfluidics, portable instruments, and smartphones. Furthermore, current challenges are emphasized and future perspectives are discussed.

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Section: Biosensors With Different Transduces For Detection Of Salmonmentioning

confidence: 99%

“…To further improve the detection sensitivity, an evaporation device that applied ionic wind flows to evaporate the droplet was developed for concentration of NAEBs‐bound Salmonella . Single CFU of Salmonella could be detected when 30 μL of sample volume was used (Su et al., 2019).…”

Section: Biosensors With Different Transduces For Detection Of Salmonmentioning

confidence: 99%

Biosensors for rapid detection of Salmonella in food: A review

Shen

2020

Comp Rev Food Sci Food Safe

136

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“…Our group has successfully developed electrohydrodynamic micro-concentrators to trap a small sample aliquot (30–50 µL) containing pathogenic bacteria including Neisseria and Salmonella using centrifugal flows generated by corona discharge-induced wind [ 24 , 25 ]. By hanging a needle electrode with a high AC voltage (≥1 kV) of 50 kHz about 3 mm above the surface of a sample droplet on an electrically grounded slide, we can create large amounts of plasma ions at the needle tip to collide with electroneutral air molecules, generating the airflow known as ionic wind.…”

Section: Introductionmentioning

confidence: 99%

“…These NAEBs have been demonstrated to provide a ultrahigh-sensitive SERS signal from even a single NAEB, due to the Raman molecule-induced “hot-spots” in the nanoaggregates. [ 26 ] Raman signals from the bound tags on bacteria can be employed to quantify pathogen cell numbers even down to the single bacterium level [ 18 , 24 , 25 , 27 , 28 ]. When a sample droplet of 30 μL was evaporated in half an hour using ionic wind to concentrate the collected bacteria, detection of a few cells of Salmonella was successfully accomplished.…”

Section: Introductionmentioning

confidence: 99%

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Integration of a Thermoelectric Heating Unit with Ionic Wind-Induced Droplet Centrifugation Chip to Develop Miniaturized Concentration Device for Rapid Determination of Salmonella on Food Samples Using Antibody-Functionalized SERS Tags

Chen

Wang

et al. 2020

Sensors

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When a centrifugation-enriched sample of 100 μL containing the surface-enhanced Raman scattering (SERS) tag-bound bacteria (Salmonella in this study) is siphoned onto a glass slide next to an embedded thermoelectric heating chip, such a sessile droplet is quickly evaporated. As the size of the sample droplet is significantly reduced during the heating process, ionic wind streams from a corona discharge needle, stationed above the sample, sweep across the liquid surface to produce centrifugal vortex flow. Tag-bound Salmonella in the sample are then dragged and trapped at the center of droplet bottom. Finally, when the sample is dried, unlike the “coffee ring” effect, the SERS tag-bound Salmonella is concentrated in one small spot to allow sensitive detection of a Raman signal. Compared with our previous electrohydrodynamic concentration device containing only a corona discharge needle, this thermoelectric evaporation-assisted device is more time-effective, with the time of concentrating and drying about 100 μL sample reduced from 2 h to 30 min. Hence, sample throughput can be accelerated with this device for practical use. It is also more sensitive, with SERS detection of a few cells of Salmonella in neat samples achievable. We also evaluated the feasibility of using this device to detect Salmonella in food samples without performing the culturing procedures. Having spiked a few Salmonella cells into ice cubes and lettuce leaves, we use filtration and ultracentrifugation steps to obtain enriched tag-bound Salmonella samples of 200 μL. After loading an aliquot of 100 μL of sample onto this concentration device, the SERS tag signals from samples of 100 g ice cubes containing two Salmonella cells and 20 g lettuce leaf containing 5 Salmonella cells can be successfully detected.

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Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

et al. 2020

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The rapid, highly sensitive, and accurate detection of bacteria is the focus of various fields, especially food safety and public health. Surface-enhanced Raman spectroscopy (SERS), with the advantages of being fast, sensitive, and nondestructive, can be used to directly obtain molecular fingerprint information, as well as for the on-line qualitative analysis of multicomponent samples. It has therefore become an effective technique for bacterial detection. Within this progress report, advances in the detection of bacteria using SERS and other compatible techniques are discussed in order to summarize its development in recent years. First, the enhancement principle and mechanism of SERS technology are briefly overviewed. The second part is devoted to a label-free strategy for the detection of bacterial cells and bacterial metabolites. In this section, important considerations that must be made to improve bacterial SERS signals are discussed. Then, the label-based SERS strategy involves the design strategy of SERS tags, the immunomagnetic separation of SERS tags, and the capture of bacteria from solution and dye-labeled SERS primers. In the third part, several novel SERS compatible technologies and applications in clinical and food safety are introduced. In the final part, the results achieved are summarized and future perspectives are proposed.

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Electrohydrodynamically enhanced drying droplets for concentration of Salmonella bacteria prior to their detections using antibody-functionalized SERS-reporter submicron beads

Cited by 14 publications

References 31 publications

Biosensors for rapid detection of Salmonella in food: A review

Biosensors for rapid detection of Salmonella in food: A review

Integration of a Thermoelectric Heating Unit with Ionic Wind-Induced Droplet Centrifugation Chip to Develop Miniaturized Concentration Device for Rapid Determination of Salmonella on Food Samples Using Antibody-Functionalized SERS Tags

Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

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