Electrohydrodynamic printing of sub-microscale fibrous architectures with improved cell adhesion capacity

Zhang, Bing; He, Jiankang; Qi, Lei; Li, Dichen

doi:10.1080/17452759.2019.1662991

Cited by 28 publications

(21 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The voltage, feeding rate, and stage moving speed were fixed at 4.6 kV, 30 µl/h, and 35 mm/s, respectively. To fabricate the sub-microscale fibrous architectures, solution-based EHD printing process was employed[ 20 ]. The nozzle gauge was 34G and the nozzle to collector distance was set as 2 mm.…”

Section: Methodsmentioning

confidence: 99%

“…We previously developed a solution-based EHD printing method for the fabrication of sub-microscale fibrous architectures[ 20 ]. The effect of sub-microscale fibers (about 0.5 μm) on rat myocardial cells was preliminarily investigated, with the results indicating that sub-microscale fibers could enhance cellular adhesion and orientation, whereas the effect of EHD-printed sub-microscale fibrillar architectures on bone cells’ adhesion patterns, spreading morphologies, growth, migration, and osteogenic differentiation was not clear.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Attachment and Collagen Type I Deposition of MC3T3-E1 Cells via Electrohydrodynamic Printed Sub-Microscale Fibrous Architectures

Hu¹,

Meng²,

Zhou³

et al. 2022

ijb

Self Cite

View full text Add to dashboard Cite

Micro/sub-microscale fibrillar architectures of extracellular matrix play important roles in regulating cellular behaviors such as attachment, migration, and differentiation. However, the interactions between cells and organized micro/sub-microscale fibers have not been fully clarified yet. Here, the responses of MC3T3-E1 cells to electrohydrodynamic (EHD) printed scaffolds with microscale and/or sub-microscale fibrillar architectures were investigated to demonstrate their potential for bone tissue regeneration. Fibrillar scaffolds were EHD-fabricated with microscale (20.51 ± 1.70 μm) and/or sub-microscale (0.58 ± 0.51 μm) fibers in a controlled manner. The in vitro results showed that cells exhibited a 1.25-fold increase in initial attached cell number and 1.17-fold increase in vinculin expression on scaffolds with micro/sub-microscale fibers than that on scaffolds with pure microscale fibers. After 14 days of culture, the cells expressed 1.23 folds increase in collagen type I (COL-I) deposition compared with that on scaffolds with pure microscale fibers. These findings indicated that the EHD printed sub-microscale fibrous architectures can facilitate attachment and COL I secretion of MC3T3-E1 cells, which may provide a new insight to the design and fabrication of fibrous scaffolds for bone tissue engineering.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Attachment and Collagen Type I Deposition of MC3T3-E1 Cells via Electrohydrodynamic Printed Sub-Microscale Fibrous Architectures

Hu¹,

Meng²,

Zhou³

et al. 2022

ijb

Self Cite

View full text Add to dashboard Cite

show abstract

“…writing (DIW) [20,149,150], and electrohydrodynamic printing (EHDP) [151][152][153][154][155][156]. Here we focus exclusively on DIW and EHDP, as these two methods are the most promising for micro and nanoscale fabrication.…”

Section: Inkjet Printingmentioning

confidence: 99%

3D Nanophotonic device fabrication using discrete components

Melzer

McLeod

2020

Nanophotonics

View full text Add to dashboard Cite

AbstractThree-dimensional structure fabrication using discrete building blocks provides a versatile pathway for the creation of complex nanophotonic devices. The processing of individual components can generally support high-resolution, multiple-material, and variegated structures that are not achievable in a single step using top-down or hybrid methods. In addition, these methods are additive in nature, using minimal reagent quantities and producing little to no material waste. In this article, we review the most promising technologies that build structures using the placement of discrete components, focusing on laser-induced transfer, light-directed assembly, and inkjet printing. We discuss the underlying principles and most recent advances for each technique, as well as existing and future applications. These methods serve as adaptable platforms for the next generation of functional three-dimensional nanophotonic structures.

show abstract

“…13−15 Both pore size and fiber stacking structure can be precisely controlled by EHDP, and the diameter of the deposited fiber can be adjusted from hundreds of nanometers to a few micrometers, closely resembling the dimensions of the ECM microenvironment. 16,17 EHDP shares a similar working principle with near-field electrospinning that employs a high electric field to induce the ejection of the fiber from either polymer melts or solution. 18,19 A few synthetic biopolymers, such as poly-ε-caprolactone (PCL), 20,21 polyethylene oxide (PEO), 22 and polylactic acid, 23 have been utilized as biomaterial inks in EHDP for scaffold fabrication.…”

Section: Introductionmentioning

confidence: 99%

Engineered Nanotopography on the Microfibers of 3D-Printed PCL Scaffolds to Modulate Cellular Responses and Establish an In Vitro Tumor Model

Jing

Wang

Leng

et al. 2021

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Scaffold-based three-dimensional (3D) cell culture systems have gained increased interest in cell biology, tissue engineering, and drug screening fields as a replacement of twodimensional (2D) monolayer cell culture and as a way to provide biomimetic extracellular matrix environments. In this study, microscale fibrous scaffolds were fabricated via electrohydrodynamic printing, and nanoscale features were created on the fiber surface by simply leaching gliadin of poly(ε-caprolactone) (PCL)/ gliadin composites in ethanol solution. The microstructure of the printed scaffolds could be precisely controlled by printing parameters, and the surface nanotopography of the printed fiber could be tuned by varying the PCL/gliadin ratios. By seeding mouse embryonic fibroblast (NIH/3T3) cells and human nonsmall cell lung cancer (A549) cells on the printed scaffolds, the cellular responses showed that the fiber nanotopography on printed scaffolds efficiently favored cell adhesion, migration, proliferation, and tissue formation. Quantitative analysis of the transcript expression levels of A549 cells seeded on nanoporous scaffolds further revealed the upregulation of integrin-β1, focal adhesion kinase, Ki-67, E-cadherin, and epithelial growth factor receptors over what was observed in the cells grown on the pure PCL scaffold. Furthermore, a significant difference was found in the relevant biomarker expression on the developed scaffolds compared with that in the monolayer culture, demonstrating the potential of cancer cell-seeded scaffolds as 3D in vitro tumor models for cancer research and drug screening.

show abstract

Electrohydrodynamic printing of sub-microscale fibrous architectures with improved cell adhesion capacity

Cited by 28 publications

References 51 publications

Enhanced Attachment and Collagen Type I Deposition of MC3T3-E1 Cells via Electrohydrodynamic Printed Sub-Microscale Fibrous Architectures

Enhanced Attachment and Collagen Type I Deposition of MC3T3-E1 Cells via Electrohydrodynamic Printed Sub-Microscale Fibrous Architectures

3D Nanophotonic device fabrication using discrete components

Engineered Nanotopography on the Microfibers of 3D-Printed PCL Scaffolds to Modulate Cellular Responses and Establish an In Vitro Tumor Model

Contact Info

Product

Resources

About