Electrogenic Sodium Pump in Squid Giant Axon

Weer, Paul De; Geduldig, D.

doi:10.1126/science.179.4080.1326

Cited by 45 publications

(9 citation statements)

References 8 publications

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“…It has been well-established that inhibition of pump activity by glycosides such as ouabain or strophanthidin can further depolarize a cell (Kerkut and Thomas, 1965; Carpenter and Alving, 1968; Smith et al, 1968; Baylor and Nicholls, 1969; Sokolove and Cooke, 1971; Thomas, 1972b; Jansen and Nicholls, 1973; De Weer and Geduldig, 1973; Mat Jais et al, 1986; Doebler, 2000; Glitsch, 2001; Pulver and Griffith, 2010; Wang et al, 2012), which is consistent with the inward current produced by strophanthidin in our voltage-clamp experiments. Such depolarization could explain the observed decrease in the period in our experiments (Figure 5A) and by Tobin and Calabrese (2005).…”

Section: Resultssupporting

confidence: 90%

“…The pump is a transmembrane protein that maintains the intracellular concentrations of Na + and K + by exchanging three intracellular Na + ions for two extracellular K + ions with each cycle of ATP (adenosine triphosphate) hydrolysis (Thomas, 1972a; De Weer and Geduldig, 1973). Because the exchange of Na + and K + ions is unequal, the pump is electrogenic as it generates an outward current (Glitsch, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Na+/K+ pump interacts with the h-current to control bursting activity in central pattern generator neurons of leeches

Kueh

Barnett

Cymbalyuk

et al. 2016

eLife

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The dynamics of different ionic currents shape the bursting activity of neurons and networks that control motor output. Despite being ubiquitous in all animal cells, the contribution of the Na+/K+ pump current to such bursting activity has not been well studied. We used monensin, a Na+/H+ antiporter, to examine the role of the pump on the bursting activity of oscillator heart interneurons in leeches. When we stimulated the pump with monensin, the period of these neurons decreased significantly, an effect that was prevented or reversed when the h-current was blocked by Cs+. The decreased period could also occur if the pump was inhibited with strophanthidin or K+-free saline. Our monensin results were reproduced in model, which explains the pump’s contributions to bursting activity based on Na+ dynamics. Our results indicate that a dynamically oscillating pump current that interacts with the h-current can regulate the bursting activity of neurons and networks.DOI: http://dx.doi.org/10.7554/eLife.19322.001

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Na+/K+ pump interacts with the h-current to control bursting activity in central pattern generator neurons of leeches

Kueh

Barnett

Cymbalyuk

et al. 2016

eLife

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“…Then during the nerve impulse there would be a reduced amount of sodium conductance and hence reduced fluxes. De Weer & Gedulig (1973) have reported a small depolarization of L. pealii axons on application of strophanthidin which suggests that the effect of ouabain might be explained by such a mechanism.…”

Section: Discussionmentioning

confidence: 99%

The temperature dependence of the movement of sodium ions associated with nerve impulses

Cohen¹,

Landowne

1974

The Journal of Physiology

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“…Lithium ions apparently cannot act as a substrate for sodium pumps, and replacement of external sodium with lithium blocks the electrogenic action of such pumps (Nakajima & Takahashi, 1966;Carpenter & Alving, 1968;Rang & Ritchie, 1968;Livengood & Kusano, 1970;Sokolove & Cooke, 1971;De Weer & Geduldig, 1973). Fig.…”

Section: Resultsmentioning

confidence: 99%

Cyclic variation of potassium conductance in a burst‐generating neurone in Aplysia

Junge¹,

Stephens²

1973

The Journal of Physiology

121

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SUMMARY1. The hyperpolarization between bursts in the R 15 cell of Aplysia is accompanied by an increase in membrane slope conductance.2. The post-burst hyperpolarization can be observed with ouabain, lithium, or potassium-free solution if artificial inward current is applied. The hyperpolarization can be observed with dinitrophenol or cooling to 10°C, with no injected current. Thus, the hyperpolarization apparently is not due to the cyclic activity of an electrogenic pump.3. A reversal potential for the post-burst hyperpolarization can be demonstrated by passage of inward current during the inter-burst period. The reversal of direction of the potential depends on recent occurrence of a burst.4. The reversal potential varies with external potassium concentration, but not with chloride or sodium.5. The post-burst hyperpolarization is not blocked by external tetraethylammonium at a concentration which greatly prolongs the action potentials.6. During the onset of spike blockage by, and recovery from, calciumfree + tetrodotoxin saline, the bursts of action potentials appear to be driven by endogenous waves of membrane potential.7. The hyperpolarizing phase of the waves in calcium-free + tetrodotoxin medium is accompanied by an increased slope conductance.8. A reversal potential can be demonstrated for the hyperpolarization following a wave in calcium-free + tetrodotoxin medium by applying inward current during the interwave period.9. The waves in calcium-free + tetrodotoxin medium are blocked by ouabain but can be reinstated by artificial hyperpolarization.10. The post-burst hyperpolarization and the post-wave hyperpolarization appear to result from a periodic increase in membrane conductance, primarily to potassium ions. DOUGLAS JUNGE AND CATHY L. STEPHENS

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Electrogenic Sodium Pump in Squid Giant Axon

Cited by 45 publications

References 8 publications

Na+/K+ pump interacts with the h-current to control bursting activity in central pattern generator neurons of leeches

Na+/K+ pump interacts with the h-current to control bursting activity in central pattern generator neurons of leeches

The temperature dependence of the movement of sodium ions associated with nerve impulses

Cyclic variation of potassium conductance in a burst‐generating neurone in Aplysia

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