Na+/K+ pump interacts with the h-current to control bursting activity in central pattern generator neurons of leeches

Abstract

The dynamics of different ionic currents shape the bursting activity of neurons and networks that control motor output. Despite being ubiquitous in all animal cells, the contribution of the Na+/K+ pump current to such bursting activity has not been well studied. We used monensin, a Na+/H+ antiporter, to examine the role of the pump on the bursting activity of oscillator heart interneurons in leeches. When we stimulated the pump with monensin, the period of these neurons decreased significantly, an effect that was prevented or reversed when the h-current was blocked by Cs+. The decreased period could also occur if the pump was inhibited with strophanthidin or K+-free saline. Our monensin results were reproduced in model, which explains the pump’s contributions to bursting activity based on Na+ dynamics. Our results indicate that a dynamically oscillating pump current that interacts with the h-current can regulate the bursting activity of neurons and networks.DOI: http://dx.doi.org/10.7554/eLife.19322.001

Differential contributions of Ca2+‐activated K+ channels and Na+/K+‐ATPases to the generation of the slow afterhyperpolarization in CA1 pyramidal cells

“…Consequently, intense spike activity is expected to augment NKA current and hyperpolarize the neurons until excess intracellular Na + is pumped out of the cell. Engagement of NKA in this type of feedback inhibition was previously described in several types of neurons in addition to CA1 pyramidal cells (Jansen & Nicholls, ; Koike, Mano, Okada, & Oshima, ; Gordon, Kocsis, & Waxman, ; Morita, David, Barrett, & Barrett, ; Parker, Hill, & Grillner, ; Pulver & Griffith, ; Kim & von Gersdorff, ; Zhang, Picton, Li, & Sillar, ; Kueh, Barnett, Cymbalyuk, & Calabrese, ).…”

Regulation of Neuronal Na+/K+-ATPase by Specific Protein Kinases and Protein Phosphatases

“…This activity-dependent hyperpolarization leads to spike frequency adaptation and even to cessation of firing. Engagement of NKAs in this type of feedback inhibition was previously described in multiple types of vertebrate neurons (Koike et al, 1972;Gordon et al, 1990;Morita et al, 1993;Parker et al, 1996;Pulver and Griffith, 2010;Kim and von Gersdorff, 2012;Zhang et al, 2015;Kueh et al, 2016).…”

Vertical Finger Displacement Is Reduced in Index Finger Tapping During Repeated Bout Rate Enhancement

“…They could also focus on the effect of different durations of bouts and rest periods on the phenomenon. Results from such studies would supplement animal studies investigating post exercise excitation of rhythmic movement mediated by central pattern generators (Kueh, Barnett, Cymbalyuk, & Calabrese, 2016;Aboodarda, Copithorne, Pearcey, Button, & Power, 2015).…”