Electrogenerated Chemiluminescence DNA Biosensor Based on Hairpin DNA Probe Labeled with Ruthenium Complex

Zhang, Jing; Qi, Honglan; Li, Yan; Yang, Jia; Gao, Qiang; Zhang, Chengxiao

doi:10.1021/ac701995g

Cited by 188 publications

(90 citation statements)

References 48 publications

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“…Ru1 labelled S4 was synthesized according to reference. [36] Apparatus ECL measurements were done on MPIÀA ECL detector (Xi'an Remax Electronics, China), in which a photomultiplier tube (PMT) is biased on 900 V. Electrochemical measurements were taken with a CHI600D electrochemistry workstation (Chenhua Instruments Co., China). Gold electrode (2.0 mm diameter) was used as working electrode while a platinum wire and an Ag/AgCl (saturated KCl) were used as counter electrode and reference electrode, respectively.…”

Section: Experimental Section Reagentsmentioning

confidence: 99%

Highly Sensitive Electrochemiluminescence Assay for Cardiac Troponin I and Adenosine Triphosphate by using Supersandwich Amplification and Bifunctional Aptamer

Liu

et al. 2017

ChemElectroChem

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A highly sensitive electrochemiluminescence (ECL) assay was developed for sequential detection of cardiac troponin I (cTnI) and adenosine triphosphate (ATP) through supersandwich amplification and bifunctional aptamer. The bifunctional aptamer (S1) contained ATP binding aptamer and cTnI binding aptamer. A gold electrode was modified with the specific peptide of cTnI through a self-assembly technique. A sandwichtype conjugate (peptide < cTnI > S1) was formed when the peptide-modified electrode was successively reacted with cTnI and S1. Then, hybridizations between S1 and two ss-DNA auxiliary probes, in which one is complementary with ATP binding aptamer and the other is ATP binding aptamer, led to the formation of long-range ds-DNA on the electrode surface. In the presence of ECL indicator Ru(phen) 3 2 + , a large amount of Ru (phen) 3 2 + was intercalated into ds-DNA grooves, resulting in amplification of the ECL signals. The ECL assay was successfully developed for the detection of cTnI in the range of 8.0 10 À13 to 1.0 10 À11 g/mL based on the increased ECL intensity. After detecting cTnI, ATP was detected in the range of 30 to 500 nM, based on switching structures of aptamers from ds-DNA to ss-DNA/target complex. A low detection limit of 0.3 pg/mL and 10 nM for cTnI and ATP, respectively, was obtained. The employment of a bifunctional aptamer probe and supersandwich signal amplification is promising for the sensitive detection of multiple targets.

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Section: Experimental Section Reagentsmentioning

confidence: 99%

Highly Sensitive Electrochemiluminescence Assay for Cardiac Troponin I and Adenosine Triphosphate by using Supersandwich Amplification and Bifunctional Aptamer

Liu

et al. 2017

ChemElectroChem

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“…2b, it can be seen that both ECL peaks appear at near + 900 mV, which is similar with that (+900 mV) in ref. [35], indicating that the ECL behavior of Ru-peptide2 is similar to that of Ru(bpy) 2 (dcbpy)NHS and Ru(bpy) 2 (dcbpy)NHS is attached to peptide2.…”

Section: Characterization Of the Ecl Probementioning

confidence: 89%

Sensitive electrogenerated chemiluminescence peptide-based biosensor for the determination of troponin I with gold nanoparticles amplification

Meng

Qiu

et al. 2013

Gold Bull

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A sensitive electrogenerated chemiluminescence (ECL) peptide-based biosensor was fabricated for the determination of troponin I (TnI) by employing gold nanoparticles as amplification platform. Two specific peptides including peptide1 with a sequence of CFYSHSFHENWPS and peptide2 with a sequence of FYSHSFHENWPSK were employed as capture peptide and report peptide, respectively. The peptide2 was labeled with ruthenium bis(2,2′-bipyridine) (2,2′-bipyridine-4,4′-dicarboxylic acid)-N-hydroxysuccinimide ester (Ru(bpy) 2 (dcbpy)NHS) at NH 2 -containing lysine via acylation reaction and utilized as the ECL probe. Gold nanoparticles were electrodeposited onto gold electrode and used as an amplification platform. The peptide-based biosensor was fabricated by self-assembling peptide1 onto the surface of gold nanoparticles-modified gold electrode through a thiolcontaining cysteine at the end of the peptide1. When the biosensor reacted with target TnI, and then incubated with the ECL probe, a strong ECL response was electrochemically generated. The ECL intensity is directly proportional to the logarithm of the concentration of TnI in the range from 1 to 300 pg/mL. The biosensor employing gold nanoparticles as amplification platform shows high sensitivity for the detection of TnI with a detection limit of 0.4 pg/mL (S/N =3). Moreover, the biosensor is successfully applied to analysis of TnI in human serum sample. This work demonstrates that the combination of a highly binding peptide with nanoparticle amplification is a great promising approach for the design of ECL biosensor.

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“…And the chronoamperometry (CA) was determined by measuring the ECL signal. [42] Cell imaging: Fluorescence imaging was conducted with a fluorescence microscope setup consisting of an Nikon E 800 inverted microscope with an Nikon Digital sight DS-U1 camera. The fluorescent NPs were excited at 400 nm, and emission was detected at 545 nm.…”

Section: Electrochemiluminescence (Ecl) Detectionmentioning

confidence: 99%

Electrochemical and Electrochemiluminescence Determination of Cancer Cells Based on Aptamers and Magnetic Beads

Ding

Zhang

2010

Chemistry A European J

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The electrochemical and electrochemiluminescence (ECL) detection of cell lines of Burkitt’s lymphoma (Ramos) by using magnetic beads as the separation tool and high‐affinity DNA aptamers for signal recognition is reported. Au nanoparticles (NPs) bifunctionalized with aptamers and CdS NPs were used for electrochemical signal amplification. The anodic stripping voltammetry technology employed for the analysis of cadmium ions dissolved from CdS NPs on the aggregates provided a means to quantify the amount of the target cells. This electrochemical method could respond down to 67 cancer cells per mL with a linear calibration range from 1.0×102 to 1.0×105 cells mL−1, which shows very high sensitivity. In addition, the assay was able to differentiate between target and control cells based on the aptamer used in the assay, indicating the wide applicability of the assay for diseased cell detection. ECL detection was also performed by functionalizing the signal DNA, which was complementary to the aptamer of the Ramos cells, with tris(2,2‐bipyridyl) ruthenium. The ECL intensity of the signal DNA, replaced by the target cells from the ECL probes, directly reflected the quantity of the amount of the cells. With the use of the developed ECL probe, a limit of detection as low as 89 Ramos cells per mL could be achieved. The proposed methods based on electrochemical and ECL should have wide applications in the diagnosis of cancers due to their high sensitivity, simplicity, and low cost.

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Electrogenerated Chemiluminescence DNA Biosensor Based on Hairpin DNA Probe Labeled with Ruthenium Complex

Cited by 188 publications

References 48 publications

Highly Sensitive Electrochemiluminescence Assay for Cardiac Troponin I and Adenosine Triphosphate by using Supersandwich Amplification and Bifunctional Aptamer

Highly Sensitive Electrochemiluminescence Assay for Cardiac Troponin I and Adenosine Triphosphate by using Supersandwich Amplification and Bifunctional Aptamer

Sensitive electrogenerated chemiluminescence peptide-based biosensor for the determination of troponin I with gold nanoparticles amplification

Electrochemical and Electrochemiluminescence Determination of Cancer Cells Based on Aptamers and Magnetic Beads

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