Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein

Electrodeposition of Silver Amalgam on Thin Gold Film Electrodes for Voltammetric Detection of 4‐Nitrophenol and DNA Labeled with Osmium Tetroxide‐Bipyridine Complex

“…Among the above mention procedures, very promising results have been obtained by the electrochemical deposition of silver amalgam particles (AgAPs) onto various conductive supporting materials. To date, indium tin oxide (ITO) transparent electrode [25], glassy carbon (GC) [26] or pyrolytic graphite electrodes (PGE) [27] have already been used as substrates, and the respective electrodeposition conditions have been optimized. ITO and PGE electrodes decorated by AgAPs were found to be suitable in organic nitro compounds' voltammetric and spectroelectrochemical detection.…”

“…The GC with electrodeposited AgAP was used to indirectly determine cysteine, glutathione, and metallothioneins by measuring the catalytic hydrogen evolution signal according to the Brdička reaction using cobalt as a catalyst [26]. Thanks to preferential adsorption of fluorescently labeled calf thymus DNA and green fluorescent protein (GFP) on AgAP electrodeposited on PGE, intrinsic DNA redox signals (cathodic peak of cytosine and adenine, and anodic peak of guanine's reduction products) and catalytic peak H offered by GFP could thus be observed [25,27]. Bovine serum albumin protein's analogical CHER response could be observed down to 5 nmol.l À 1 using ultrathin (< 1 μm) silver solid amalgam electrode arrays, prepared by vacuum silver metal sputtering onto a glass substrate, photolithography and galvanic mercury amalgam formation [28].…”

Electrodeposition of Silver Amalgam Particles on Screen-Printed Silver Electrodes in Voltammetric Detection of 4-Nitrophenol and Artificial Nucleosides dTPT3 and d5SICS

“…Electrodeposition of silver amalgam particles (AgAPs) on various substrates was found to be perspective approach for development of novel (spectro-)electroanalytical systems applicable in detection of electrochemically reducible compounds, nucleic acids or proteins [1][2][3][4] . Moreover, AgAPs were confirmed as a suitable material for introduction of plasmonic functionalities into photochemical and spectro-electrochemical systems [5] .…”

“…Surface modification of scree-printing electrodes by electrodeposited AgAPs has never been studied before according to our best of knowledge. Since AgAPs have proven to be suitable for the surface modification of transparent indium-tin oxide (ITO) [1] , vapor deposited golden thin film (vAuE) [2] or pyrolytic graphite (PG) [3] , herein in-house fabricated screen-printed silver electrode with silver composite working electrode (SPAgE) has been selected as novel substrate for AgAPs deposition. This metal silver based substrate should also minimize risk of mercury contamination, thank to high mercury affinity to the silver substrate, and thus increased mechanical stability and about two orders of magnitude lowered mercury vapor pressure [7] .…”

“…This metal silver based substrate should also minimize risk of mercury contamination, thank to high mercury affinity to the silver substrate, and thus increased mechanical stability and about two orders of magnitude lowered mercury vapor pressure [7] . For all the above mention substrates, the AgAPs modification has significantly facilitated electroreduction of organic nitro compounds [1] and additionally allowed detection of labeled [2] or even unlabeled nucleic acids [3] or proteins [4] . Analogical effects are expected in case of AgAPs electrodeposition on SPAgE, but detail study and optimization are needed.…”