Electrodeless coating polypyrrole on chitosan grafted polyurethane with functionalized multiwall carbon nanotubes electrospun scaffold for nerve tissue engineering

Shrestha, Sita; Shrestha, Bishnu Kumar; Kim, Jeong In; Ko, Sung Won; Park, Chan Hee; Kim, Cheol Sang

doi:10.1016/j.carbon.2018.04.064

Cited by 101 publications

(47 citation statements)

References 54 publications

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“…Pre-osteoblast (MC3T3-E1) cell lines derived from mouse Clone 4, ATCC CRL-5293 were first cultured in Minimum Essential Medium (α-MEM, Hyclone ™ , Thermo Fisher Scientific, Logan, UT, USA) under incubation at 37 • C and 5% carbon dioxide (CO 2 ), in accordance with the previously reported literature [29,30]. Briefly, cells at almost 85% confluency were harvested, then seeded (under UV light for overnight) onto the center of sterile tissue culture plate (TCP) and Mg-OH-AA-BSA scaffold at a density of 3 × 10 4 cells/well.…”

Section: In Vitro Cells Interactionmentioning

confidence: 99%

Covalent Surface Functionalization of Bovine Serum Albumin to Magnesium Surface to Provide Robust Corrosion Inhibition and Enhance In Vitro Osteo-Inductivity

Lee

Shrestha

et al. 2020

Polymers

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Herein, we describe precisely a covalent modification of pure magnesium (Mg) surface and its application to induce in vitro osteogenic differentiation. The new concept of a chemical bonding method is proposed for developing stable chemical bonds on the Mg surface through the serial assembly of bioactive additives that include ascorbic acid (AA) and bovine serum albumin (BSA). We studied both the physicochemical and electrochemical properties using scanning electron microscopy and other techniques to confirm how the covalent bonding of BSA on Mg can, after coating, significantly enhance the chemical stability of the substrate. The modified Mg-OH-AA-BSA exhibits better anti-corrosion behavior with high corrosion potential (Ecorr = −0.96 V) and low corrosion current density (Icorr = 0.2 µA cm−2) as compared to the pure Mg (Ecorr = −1.46 V, Icorr = 10.42 µA cm−2). The outer layer of BSA on Mg protects the fast degradation rate of Mg, which is the consequence of the strong chemicals bonds between amine groups on BSA with carboxylic groups on AA as the possible mechanism of peptide bonds. Collectively, the results suggest that the surface-modified Mg provides a strong bio-interface, and enhances the proliferation and differentiation of pre-osteoblast (MC3T3-E1) cells through a protein–lipid interaction. We therefore conclude that the technique we describe provides a cost-effective and scalable way to generate chemically stable Mg surface that inherits a biological advantage in orthopedic and dental implants in clinical applications.

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Section: In Vitro Cells Interactionmentioning

confidence: 99%

Covalent Surface Functionalization of Bovine Serum Albumin to Magnesium Surface to Provide Robust Corrosion Inhibition and Enhance In Vitro Osteo-Inductivity

Lee

Shrestha

et al. 2020

Polymers

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“…Likewise, Niu et al reported that scaffolds from block polyurethanes based on PCL/PEG demonstrated much better regeneration behaviour than PCL, silicone tube due to the presence of biochemical and topographic cues and highly surface-area porous [131]. It has been reported that coating of polyurethane with conductive polymers or blending with other polymers has resulted in improvement of the physicochemical properties such as mechanical strength, cytocompatibility, and electrical conductivity for nerve tissue engineering [132].…”

Section: Pcl Polymermentioning

confidence: 99%

Tailoring synthetic polymeric biomaterials towards nerve tissue engineering: a review

Amani

Kazerooni

Hassanpoor

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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The nervous system is known as a crucial part of the body and derangement in this system can cause potentially lethal consequences or serious side effects. Unfortunately, the nervous system is unable to rehabilitate damaged regions following seriously debilitating disorders such as stroke, spinal cord injury and brain trauma which, in turn, lead to the reduction of quality of life for the patient. Major challenges in restoring the damaged nervous system are low regenerative capacity and the complexity of physiology system. Synthetic polymeric biomaterials with outstanding properties such as excellent biocompatibility and non-immunogenicity find a wide range of applications in biomedical fields especially neural implants and nerve tissue engineering scaffolds. Despite these advancements, tailoring polymeric biomaterials for design of a desired scaffold is fundamental issue that needs tremendous attention to promote the therapeutic benefits and minimize adverse effects. This review aims to (i) describe the nervous system and related injuries. Then, (ii) nerve tissue engineering strategies are discussed and (iii) physiochemical properties of synthetic polymeric biomaterials systematically highlighted. Moreover, tailoring synthetic polymeric biomaterials for nerve tissue engineering is reviewed.

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“…MOFs are also used for photodynamic therapies, sensing or bio-imaging agents [34]. Furthermore, it was shown that polypyrrole and its electrical composites were greatly researched for biomedical applications such as tissue regeneration, drug delivery systems, and electrical stimulation therapy owing to their low toxicity, ease of preparation, redox property, and high conductivity [35,36].…”

Section: Effect Of Ppy Co-mof Ppy/x%co-mof On the Viability Of 3t3 mentioning

confidence: 99%

Fabrication and biocompatibility assessment of polypyrrole/cobalt(II) metal-organic frameworks nanocomposites

Mehrabani¹,

Ansari-Asl²,

Rostamzadeh³

et al. 2020

Turk J Chem

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Nowadays, metal-organic frameworks (MOFs) have emerged as promising tools for different biological applications and therefore, efforts are ongoing to develop more biocompatible MOFs-based nanocomposites. We aimed to fabricate some new conductive nanocomposites of polypyrrole and cobalt-MOF with different weight percentages (PPy/x%Co-MOF) using the solution mixing method and characterize them through FT-IR (Fourier-transform infrared), PXRD (powder X-ray diffraction), SEM (scanning electron microscope), and TEM (transmission electron microscope) techniques. The biocompatibility of nanocomposites was assessed by haemolytic, cytotoxic, and quantitative reverse transcription PCR (qRT-PCR) assays. FT-IR and PXRD results revealed that nanocomposites consisted of pure MOFs and PPy. Moreover, SEM results indicated their spherical morphology along with an average diameter of 190 nm. (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed a concentration, and percentagedependent cytotoxic effect of the nanocomposites on some cell lines including 3T3 fibroblasts, MCF-7, and J774.A1 macrophages. Haematological toxicity of PPy/x%Co-MOF composites was less than 7% in most concentrations. Furthermore, PPy/x%Co-MOF composites did not show any significant effect on the expression of cyclooxygenase − 2 (COX-2) and inducible nitric oxide synthase (iNOS) genes. In sum, regarding the haemolytic, proinflammatory, and cytotoxic tests, prepared nanocomposite demonstrated the reasonable in vitro biocompatibility which may be considered as a hopeful platform for further investigations including clinical applications.

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Electrodeless coating polypyrrole on chitosan grafted polyurethane with functionalized multiwall carbon nanotubes electrospun scaffold for nerve tissue engineering

Cited by 101 publications

References 54 publications

Covalent Surface Functionalization of Bovine Serum Albumin to Magnesium Surface to Provide Robust Corrosion Inhibition and Enhance In Vitro Osteo-Inductivity

Covalent Surface Functionalization of Bovine Serum Albumin to Magnesium Surface to Provide Robust Corrosion Inhibition and Enhance In Vitro Osteo-Inductivity

Tailoring synthetic polymeric biomaterials towards nerve tissue engineering: a review

Fabrication and biocompatibility assessment of polypyrrole/cobalt(II) metal-organic frameworks nanocomposites

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