Electrocoating carbon fiber microelectrodes with Nafion improves selectivity for electroactive neurotransmitters

Brazell, M.P.; Kasser, Richard J.; Renner, Kenneth J.; Feng, Jianrong; Moghaddam, Bita; Adams, Ralph N.

doi:10.1016/0165-0270(87)90011-2

Cited by 127 publications

(72 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pt-Nafion ® (5/2) sensors incorporate an annealed form of the perfluorinated sulfonic acid ionomer on the electrode surface which has demonstrated excellent rejection characteristics previously [8]. In the past Nafion ® has been employed in electrochemical sensor design for its dual ability as both a cation exchange polymer [32][33][34][35] and an anionic rejection layer [4][5][6]. However, the described Nafion ® pre-coat method displays both anionic and cationic rejection characteristics while facilitating the diffusion of gaseous molecules such as NO and O2 to the electrode surface.…”

Section: Shelf-life Studies-effect Of Repeated Aa Calibrationsmentioning

confidence: 99%

Ascorbic Acid Rejection Characteristics of Modified Platinum Electrodes: A Shelf Life Investigation

Wynne

Finnerty

2015

Chemosensors

View full text Add to dashboard Cite

Ascorbic acid (AA) is the principle interferent present in brain extracellular fluid that can inhibit the ability of electrochemical sensors to selectively detect a particular analyte of interest. Considerable efforts have been made in recent times to develop highly selective membrane coatings to counteract the drawbacks associated with AA interference during in vivo monitoring. The primary objective of the work described within was to investigate the long term effect of storing such selective membranes, i.e., Nafion ® andPoly-o-phenylenediamine (PPD) under different conditions and how exposing them to repeated calibration protocols compromises the membranes ability to reject AA. Four different modified platinum (Pt) electrodes, Pt-PPD, Pt-Nafion ® (5/2), Pt-Nafion ® (1/2)-PPD, and Pt-Nafion ® (2/1)-PPD, stored at 4 °C demonstrated deterioration of the polymers integrity when exposed to repeated calibrations. On the contrary, exposing the same four electrode types to single calibrations confirmed excellent retention of AA rejection characteristics. Pt-PPD electrodes were then exposed to varying storage conditions and calibrated against AA on day 1, day 56 and day 168. Storing the Pt-PPD electrodes at 4 °C/N2 saturated glass container demonstrated retention of AA rejection characteristics after day 168. These results have clearly elucidated the optimum storage conditions for Pt-Nafion ® and Pt-PPD modified electrodes.

show abstract

Section: Shelf-life Studies-effect Of Repeated Aa Calibrationsmentioning

confidence: 99%

Ascorbic Acid Rejection Characteristics of Modified Platinum Electrodes: A Shelf Life Investigation

Wynne

Finnerty

2015

Chemosensors

View full text Add to dashboard Cite

show abstract

“…The most problematic species is AA because its basal extracellular concentration is higher than that of DA and because stress has been reported to elevate (striatal) levels of AA (Miele et al, 1994). Repeatedly coating the electrode surface with Nafion is one of several procedures that has been shown to substantially reduce contributions by AA and other anionic species, including the DA metabolite DOPAC (Gerhardt et al, 1984;Nagy et al, 1985;Brazell et al, 1987;Crespi and Mobius, 1992). The electrodes used in the present study had a mean (ϮSEM) DA/AA selectivity ratio of 3780:1 (Ϯ234:1); thus, the Nafion treatment would significantly reduce a potential contribution of AA to the increases in signal reported here.…”

Section: Mpfc Da Response To Stressmentioning

confidence: 99%

Influence of Naturally Occurring Variations in Maternal Care on Prepulse Inhibition of Acoustic Startle and the Medial Prefrontal Cortical Dopamine Response to Stress in Adult Rats

Zhang¹,

Chretien²,

Meaney³

et al. 2005

J. Neurosci.

140

View full text Add to dashboard Cite

In rats, naturally occurring variations in maternal care contribute to the development of individual differences in the behavioral and neuroendocrine responses to stress during adulthood. The dopamine (DA) projection to the medial prefrontal cortex (mPFC) plays an important role in mediating stress responsivity and is thought to be involved also in regulating sensorimotor gating. In the present study, we compared prepulse inhibition (PPI) of acoustic startle as well as the left and right mPFC DA stress responses in the adult offspring of high-and low-licking/grooming (LG) dams. Our data indicate that the offspring of low-LG animals are impaired on measures of PPI compared with high-LG animals. We also observed in low-LG animals a significant blunting of the mPFC DA stress responses that was lateralized to the right hemisphere, whereas in high-LG animals, the left and right mPFC DA stress responses were equally attenuated. Although mPFC levels of DA transporter did not differ between the two groups of animals, mPFC levels of catechol-O-methyl transferase immunoreactivity of low-LG animals were significantly lower than those of high-LG animals. These data provide evidence that variations in maternal care can lead to lasting changes in mPFC DA responsivity to stress and suggest the possibility that such changes in mesocorticolimbic DA function can also lead to deficits in sensorimotor gating.

show abstract

“…5-hydroxy-indole-acetic acid (5-HIAA) and uric acid (UA) also oxidize at this voltage. To decrease the contribution of 5-HIAA and UA to the 5-HT oxidation signal, all electrodes were coated with Nafion ® (Brazell et al, 1987;Rivot et al, 1995). Nafion ® is an ion exchange resin that decreases the signal of negatively charged molecular species, such as 5-HIAA and UA.…”

Section: Pretreatmentmentioning

confidence: 99%

Serotonin in the inferior colliculus fluctuates with behavioral state and environmental stimuli

Hall

Rebec

Hurley

2010

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYNeuromodulation by serotonin (5-HT) could link behavioral state and environmental events with sensory processing. Within the auditory system, the presence of 5-HT alters the activity of neurons in the inferior colliculus (IC), but the conditions that influence 5-HT neurotransmission in this region of the brain are unknown. We used in vivo voltammetry to measure extracellular 5-HT in the IC of behaving mice to address this issue. Extracellular 5-HT increased with the recovery from anesthesia, suggesting that the neuromodulation of auditory processing is correlated with the level of behavioral arousal. Awake mice were further exposed to auditory (broadband noise), visual (light) or olfactory (2,5-dihydro-2,4,5-trimethylthiazoline, TMT) stimuli, presented with food or confined in a small arena. Only the auditory stimulus or restricted movement increased the concentration of extracellular 5-HT in the IC. Changes occurred within minutes of stimulus onset, with the auditory stimulus increasing extracellular 5-HT by an average of 5% and restricted movement increasing it by an average of 14%. These findings suggest that the neuromodulation of auditory processing by 5-HT is a dynamic process that is dependent on internal state and behavioral conditions.

show abstract

Electrocoating carbon fiber microelectrodes with Nafion improves selectivity for electroactive neurotransmitters

Cited by 127 publications

References 16 publications

Ascorbic Acid Rejection Characteristics of Modified Platinum Electrodes: A Shelf Life Investigation

Ascorbic Acid Rejection Characteristics of Modified Platinum Electrodes: A Shelf Life Investigation

Influence of Naturally Occurring Variations in Maternal Care on Prepulse Inhibition of Acoustic Startle and the Medial Prefrontal Cortical Dopamine Response to Stress in Adult Rats

Serotonin in the inferior colliculus fluctuates with behavioral state and environmental stimuli

Contact Info

Product

Resources

About