Electrochemiluminescence resonance energy transfer immunoassay for alkaline phosphatase using p-nitrophenyl phosphate as substrate

Qi, Wenjing; Fu, Yuling; Zhao, Maoyu; He, Hongkun; Xue, Tian; Hu, Lianzhe; Zhang, Yan

doi:10.1016/j.aca.2019.10.073

Cited by 21 publications

(9 citation statements)

References 57 publications

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“…The linear regression equation could be described as I (the ECL intensity) = 16,959 – 1381.4 c (ALP) with a correlation coefficient of R 2 = 0.99. The limit of detection of this assay was calculated to be 0.35 U/L (S/N = 3), which is superior to those of the reported methods listed in Table S2. ,− The excellent performance of the solid-state ECL sensor illustrates the superior ECL property of CdS–Ru.…”

Section: Resultsmentioning

confidence: 76%

Highly Active Electrochemiluminescence of Ruthenium Complex Co-assembled Chalcogenide Nanoclusters and the Application for Label-Free Detection of Alkaline Phosphatase

Wang

et al. 2021

Anal. Chem.

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Rational design of electrochemiluminescence (ECL) reagents is essential for the development of ECL biosensors with superior performances. In this work, the assembly of tris(1,10-phenanthroline)ruthenium(II) [Ru(phen) 3 2+ ] and tetrahedral chalcogenide nanoclusters of [Cd 32 S 14 (SC 6 H 5 ) 38 ] 2− in the formation of complex nanoclusters (CdS−Ru) was developed, in which Ru(phen) 3 2+ was uniformly encapsulated and dispersed at a molecular level in the chalcogenide nanocluster via multiple noncovalent interactions. It was observed that the promoted ECL emission was realized by the charge transfer between the tetrahedral CdS nanocluster and Ru(phen) 32+ by the formation of the assembly complex, which was elucidated by cyclic voltammetry curves, ECL− potential curves, and in situ dynamic ECL spectra. Taking advantages of the facile charge transfer in the open framework CdS−Ru, a high ECL efficiency has been achieved with remarkable stability. Moreover, a solid-state ECL sensor based on the CdS−Ru modified electrode was fabricated for label-free detection of alkaline phosphatase (ALP) activity with a detection limit as low as 0.35 U/L and superior reproducibility. This solid-state ECL sensor also displayed favorable selectivity among various interferences and was applied for ALP activity analysis in human serum samples. These results implicated the potential applications of CdS−Ru for sensitive ECL analysis in complicated reaction systems and enlightened the rational design for self-enhanced and highly efficient ECL materials.

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Section: Resultsmentioning

confidence: 76%

Highly Active Electrochemiluminescence of Ruthenium Complex Co-assembled Chalcogenide Nanoclusters and the Application for Label-Free Detection of Alkaline Phosphatase

Wang

et al. 2021

Anal. Chem.

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“…A number of diverse methods and techniques, including colorimetric assay, electrochemistry, chromatography, photometric assay, photoelectrochemical assay and surface-enhanced Raman scattering methods, have been developed to date to detect the concentration of ALP that catalyzes the dephosphorylation process of different substrates [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. However, these traditional methods inevitably suffer from one or more limitations, such as time-consuming procedures, poor sensitivity, exorbitant material requirement, and use of complex devices.…”

Section: Introductionmentioning

confidence: 99%

An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles

et al. 2021

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As an important DNA 3′-phosphatase, alkaline phosphatase can repair damaged DNA caused by replication and recombination. It is essential to measure the level of alkaline phosphatase to indicate some potential diseases, such as cancer, related to alkaline phosphatase. Here, we designed a simple and fast method to detect alkaline phosphatase quantitively. When alkaline phosphatase is present, the resulting poly T-DNA with a 3′-hydroxyl end was cleaved by exonuclease I, prohibiting the formation of fluorescent copper nanoparticles. However, the fluorescent copper nanoparticles can be monitored with the absence of alkaline phosphatase. Hence, we can detect alkaline phosphatase with this turn-off strategy. The proposed method is able to quantify the concentration of alkaline phosphatase with the LOD of 0.0098 U/L. Furthermore, we utilized this method to measure the effects of inhibitor Na3VO4 on alkaline phosphatase. In addition, it was successfully applied to quantify the level of alkaline phosphatase in human serum. The proposed strategy is sensitive, selective, cost effective, and timesaving, having a great potential to detect alkaline phosphatase quantitatively in clinical diagnosis.

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“…It is well-known that the composites are closely connected with signal amplification and improvement of the affinity on bioactive materials. , Notably, magnetic nanocomposites could greatly improve the performance of electrochemical sensors . With controllable design and surface modification, magnetic nanocomposites could be used in the fabrication of a unique biosensor.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Aptasensor of Carcinoembryonic Antigen Based on Concanavalin A-Functionalized Magnetic Copper Silicate Carbon Microtubes and Gold-Nanocluster-Assisted Signal Amplification

Zheng

Wang

Song

et al. 2020

ACS Appl. Nano Mater.

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An ultrasensitive electrochemical sensor was developed for the quantitative determination of carcinoembryonic antigen (CEA), which is often utilized as a tumor marker and a prognostic indicator. In the paper, first, copper silicate integrated with nitrogen-doped magnetic carbon microtubes (NCMTs@ Fe 3 O 4 @Cusilicate) was synthesized and employed to enrich concanavalin A (ConA), which can be used as an affinity probe to selectively capture CEA. Through chemical chelation between a copper ion and Con A, the combination of Cusilicate/ConA was greatly improved. Furthermore, gold nanoclusters (AuNCs) immobilized with aptamers were provided for signal amplification. Thus, in the presence of the target protein, CEA, in a sandwich manner, was formed, and AuNCs were gathered on the electrode through a magnetic field. Electrochemical oxidation of AuNCs to AuCl 4 − was performed, and immediately differential pulse voltammetry was performed, resulting in an electrochemical response because of the reduction of AuCl 4 − . On the basis of these merits, the constructed sensing system provided a ultrasensitive detection scheme for CEA with a linear range from 0.03 to 6.00 ng•mL −1 and a low detection limit of 5.38 pg•mL −1 . In addition, the proposed strategy was employed to detect CEA in serum, which showed excellent correlations with standard immunoassay. The excellent performance indicated that the proposed sensor has significant potential for clinical diagnosis.

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Electrochemiluminescence resonance energy transfer immunoassay for alkaline phosphatase using p-nitrophenyl phosphate as substrate

Cited by 21 publications

References 57 publications

Highly Active Electrochemiluminescence of Ruthenium Complex Co-assembled Chalcogenide Nanoclusters and the Application for Label-Free Detection of Alkaline Phosphatase

Highly Active Electrochemiluminescence of Ruthenium Complex Co-assembled Chalcogenide Nanoclusters and the Application for Label-Free Detection of Alkaline Phosphatase

An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles

Electrochemical Aptasensor of Carcinoembryonic Antigen Based on Concanavalin A-Functionalized Magnetic Copper Silicate Carbon Microtubes and Gold-Nanocluster-Assisted Signal Amplification

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