Electrochemiluminescence polymerase chain reaction detection of genetically modified organisms

Applications of Electrochemiluminescence

“…ECL detection method combined with bead-based PCR-amplified DNA-aptamers has been utilized for the detection of anthrax spores [141]. Bead-based PCR-ECL system has also been applied to the detection of various genes from viruses and diseases in human blood and serum [142][143][144] and plants [145], genetically modified organisms such as vegetables [146], and the measurement of DNA helicase activity of Escherichia coli DNA [147].…”

Miniaturized polymerase chain reaction device for rapid identification of genetically modified organisms

“…Although PCR is one of the most timeconsuming and labor-intensive processes for genetic analyses, however, this method is indispensable for enhancing the sensitivity of target detection. It has been reported that a large number of GMOs share the same promoter of the 35S subunit of ribosomal RNA of cauliflower mosaic virus (P35S) (Lin, Chiang, & Shih, 2001;Liu, Xing, Shen, & Zhu, 2005), which can be used as a common marker for the detection of GMOs. Besides the 35S promoter sequences, bar gene, which is originally derived from the soil bacterium Streptomyces hygroscopicus and used to engineer herbicideresistant plants, was used to identify GMOs.…”

Pharmacokinetics of pefloxacin mesylate in human urine using capillary electrophoresis electrochemiluminescence detection

“…It has been widely used in the separation and analysis of illicit drugs [6,7], antibiotics [8,9] and antihistamines [10,11] etc. Among the numerous detection schemes, electrochemiluminescence (ECL), which combines the advantages and selectivity of electrochemistry and chemiluminescence [12,13], is a powerful analytical tool. ECL has become an attractive detection method for CE due to its advantages of wide dynamic range, high sensitivity and environmental green [14]; and it has been successfully used for the analysis of amine-containing compounds [15][16][17].…”