Electrochemiluminescence detection of near single DNA molecules by using quantum dots–dendrimer nanocomposites for signal amplification

Divsar, Faten; Ju, Huangxian

doi:10.1039/c1cc13592a

Cited by 52 publications

(27 citation statements)

References 28 publications

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“…Other electrochemiluminescent labels include different types of quantum dots (e.g., CdS, CdTe) and metal nanoparticle composites [e.g. PdCu@carbon, luminol‐Pt, CaCO 3 –carboxymethyl chitosan microspheres@Ag nanoparticles, CdS QD‐dendrimer nanocomposites]. Quantum dot labeling also can be achieved via an indirect labeling strategy involving biotinylated DNA and avidin‐modified quantum dots …”

Section: Electrochemiluminescent Nucleic Acidsmentioning

confidence: 99%

Nanostructured luminescently labeled nucleic acids

2016

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Important and emerging trends at the interface of luminescence, nucleic acids and nanotechnology are: (i) the conventional luminescence labeling of nucleic acid nanostructures (e.g. DNA tetrahedron); (ii) the labeling of bulk nucleic acids (e.g. single-stranded DNA, double-stranded DNA) with nanostructured luminescent labels (e.g. copper nanoclusters); and (iii) the labeling of nucleic acid nanostructures (e.g. origami DNA) with nanostructured luminescent labels (e.g. silver nanoclusters). This review surveys recent advances in these three different approaches to the generation of nanostructured luminescently labeled nucleic acids, and includes both direct and indirect labeling methods.

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Section: Electrochemiluminescent Nucleic Acidsmentioning

confidence: 99%

Nanostructured luminescently labeled nucleic acids

2016

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“…Thus, finding a way to obtain high ECL efficiency of NCs for bioanalysis is the constant driving force of this area. Many previous works have demonstrated that ECL emissions of NCs are highly dependent on their surface states, which can be changed by coupling with other nonmaterial or doping some metal ions [10][11][12][13][14][15][16][17]. Deng et al and Wang et al reported that the doping of Mn 2+ or Eu 3+ ions in the CdS NCs surface alters the surface of CdS NCs and builds a new surface state-Mn 2+ or Eu 3+ complex, also produces a maximum of 4-fold enhancement in ECL intensity [16,17].…”

Section: Introductionmentioning

confidence: 99%

A glassy carbon electrode modified with in-situ generated chromium-loaded CdS nanoprobes and heparin for ultrasensitive electrochemiluminescent determination of thrombin

2015

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We describe the in-situ electrochemical synthesis of a chromium-loaded CdS nanoprobe (CdS-Cr) for use in ultrasensitive electrochemiluminescence (ECL) detection of thrombin. A glassy carbon electrode was covered with (a) a film of electro-polymerized polyaniline nanofibers (PANI-NF), (b) the in-situ synthesized CdS-Cr nanoprobe, (c) heparin, and (d) a coating of BSA to prevent unspecific adsorption. Atomic force microscopy and scanning electron microscopy images showed the size of nanoprobe to have increased to an average size of 50±5 nm, which was larger than pure CdS nanocrystals (NCs) with their typical size of 10±2 nm. The modified glassy carbon electrode was electrochemically characterized by cyclic voltammetry and impedance spectroscopy. The thrombin-heparin interaction served as a new recognition tool for thrombin and represented an attractive alternative to respective aptamers. About 6-fold enhancement of ECL intensity (compared to pure CdS NCs) was observed in presence of persulfate. This ECL assay has a wide dynamic range (from 10 fM to 100 pM concentrations of thrombin), and a lower detection limit of 6.8 fM at 3σ. The technique for in-situ electrochemical preparation of the nanoprobe is simple, facile, and yields a sensor surface with favorable space structure, positive charge and stability.

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“…The electrochemically generated species undergo high-energy electron transfer reactions to form excited states that emit light [2,3]. Since ECL shows some remarkable features such as low background, high sensitivity, good temporal/spatial control and wide dynamic range, it has been greatly developed as one of the most frequently used transducing method for biosensor [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%