Electrochemically Stimulated Release from Liposomes Embedded in a Polyelectrolyte Multilayer

Graf, Norma; Albertini, Francesco; Petit, Tristan; Reimhult, Erik; Vörös, János; Zambelli, Tomaso

doi:10.1002/adfm.201002283

Cited by 29 publications

(48 citation statements)

References 51 publications

(48 reference statements)

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“…However, precisely controlled drug release from the thin films under mild conditions is still a challenge. A stimulus to control release of therapeutic drugs based on electrical potential could offer unique advantages, because it can easily be controlled by the nature of the stimulation conditions (the current/potential magnitude and frequency) with precise, local, continuous, and reversible features (Graf et al, 2011;Sun et al, 2013). Among the polymers, inherently conducting polymers (ICPs) have electrical and optical properties usually associated with metals, whilst retaining the advantageous mechanical properties and ease of processing at various chemical and electrochemical conditions associated with polymers (Shamaeli and Alizadeh, 2012;Wallace et al, 2009;Manbohi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Nanostructured biocompatible thermal/electrical stimuli-responsive biopolymer-doped polypyrrole for controlled release of chlorpromazine: Kinetics studies

Shamaeli

Alizadeh

2014

International Journal of Pharmaceutics

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Section: Introductionmentioning

confidence: 99%

Nanostructured biocompatible thermal/electrical stimuli-responsive biopolymer-doped polypyrrole for controlled release of chlorpromazine: Kinetics studies

Shamaeli

Alizadeh

2014

International Journal of Pharmaceutics

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“…In addition to their application as drug delivery vehicles [1,2], they are also employed in biosensing as labels [3] or as carriers for membrane proteins [4]. Further, they have recently been considered as sub-compartments in polymer capsules towards therapeutic cell mimicry [5][6][7] or as potential drug deposits embedded in polymer films on surfaces [8,9].…”

Section: Introductionmentioning

confidence: 99%

Myoblast Cell Interaction with Polydopamine Coated Liposomes

et al. 2012

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Liposomes are widely used, from biosensing to drug delivery. Their coating with polymers for stability and functionalization purposes further broadens their set of relevant properties. Poly(dopamine) (PDA), a eumelanin-like material deposited via the “self”-oxidative polymerization of dopamine at mildly basic pH, has attracted considerable interest in the past few years due to its simplicity, flexibility yet fascinating properties. Herein, we characterize the coating of different types of liposomes with PDA depending on the presence of oleoyldopamine in the lipid bilayer and the dopamine hydrochloride concentration. Further, the interaction of these coated liposomes in comparison to their uncoated counterparts with myoblast cells is assessed. Their uptake/association efficiency with these cells is determined. Further, their dose-dependent cytotoxicity with and without entrapped hydrophobic cargo (thiocoraline) is characterized. Taken together, the reported results demonstrate the potential of PDA coated liposomes as a tool in biomedical applications. Supplementary Material 13758_2011_8_MOESM1_ESM.doc (83KB)

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“…However, SMDD of small therapeutic compounds still remains a challenge, and its success is limited to specifi c examples such as the use of drug-polymer conjugates [ 11 ] or the immobilization within a carrier ( e.g. , liposomes, [ 12 ] micelles, [ 13 ] or cyclodextrins). [ 14 ] We recently reported the uptake of fl uorescent lipids by myoblast cells from a liposome-containing thin fi lm of PDA.…”

mentioning

confidence: 99%

Surface‐Adhered Composite Poly(Vinyl Alcohol) Physical Hydrogels: Polymersome‐Aided Delivery of Therapeutic Small Molecules

Hosta‐Rigau

Jensen

Fjeldsø

et al. 2012

Adv Healthcare Materials

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tissue engineering scaffolds in order to allow for more complex tissue to be cultured. [ 7 ] SMDD can be employed in two different manners: either by releasing the cargo into the surrounding media, such as the blood-stream, or by delivering the therapeutic compound to the cells adhered to the surface. In the latter case, the creation of a polymer coating which promotes both cell adhesion and proliferation is of paramount importance. To date, the sequential deposition of interacting polymer layers to create polymer thin fi lms appears to stand out since it is a versatile, inexpensive yet effi cient technique to assemble advanced coatings. [ 8 ] Successful delivery of macromolecular cargo, e.g., oligonucleotides [ 9 ] or proteins, [ 10 ] from a surface has already been documented including the implementation in vivo. However, SMDD of small therapeutic compounds still remains a challenge, and its success is limited to specifi c examples such as the use of drug-polymer conjugates [ 11 ] or the immobilization within a carrier ( e.g. , liposomes, [ 12 ] micelles, [ 13 ] or cyclodextrins). [ 14 ] We recently reported the uptake of fl uorescent lipids by myoblast cells from a liposome-containing thin fi lm of PDA. [ 15 ] Similarly, bulk hydrogels loaded with drug-containing smaller components in the form of microparticles, [ 16 ] liposomes, [ 17 ] polymersomes, [ 18 ] or micelles, [ 19 ] are termed "composite hydrogels" and were considered to gain an enhanced control over the cargo retention and release. However, to the best of our knowledge, surface-adhered composite hydrogels and their utility in SMDD has never been considered and is presented herein for the fi rst time.As sub-compartments for PVA hydrogels, we chose polymersomes, [ 20 ] colloidal vessels which self-assemble from amphiphilic block copolymers with a well-documented Surface-mediated drug delivery (SMDD) is an approach which aims to administer therapeutics to adhering or suspension cells from implantable devices or from tissue engineering scaffolds. Herein is reported the proof of concept for polymersome-aided trapping of the cytotoxic hydrophobic compound thiocoraline in a physical poly(vinyl alcohol) (PVA) hydrogel matrix and the subsequent viability of myoblast cells cultured on this surfaceadhered composite hydrogel. The ABA triblock copolymer, poly( N -acryloyl morpholine)-block -poly(cholesteryl acrylate)-block -poly( N -acryloyl morpholine) (PNAM-b -PChA-b -PNAM), is synthesized, and the self-assembly of this polymer into polymersomes is shown. The polymersomes are loaded with the model cargo fl uorescein in order to visualize the entrapping of the small payload in this composite hydrogel. To render the PVA hydrogels cell adhesive, a poly(dopamine) (PDA) coating is successfully applied to the biointerface. Finally, to demonstrate the feasibility that active cargo can be entrapped and yield a cell response, the composite hydrogels are loaded with the cytotoxic depsipeptide thiocoraline and used in SMDD. The cargo activity is ascertained via monitori...

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Electrochemically Stimulated Release from Liposomes Embedded in a Polyelectrolyte Multilayer

Cited by 29 publications

References 51 publications

Nanostructured biocompatible thermal/electrical stimuli-responsive biopolymer-doped polypyrrole for controlled release of chlorpromazine: Kinetics studies

Nanostructured biocompatible thermal/electrical stimuli-responsive biopolymer-doped polypyrrole for controlled release of chlorpromazine: Kinetics studies

Myoblast Cell Interaction with Polydopamine Coated Liposomes

Surface‐Adhered Composite Poly(Vinyl Alcohol) Physical Hydrogels: Polymersome‐Aided Delivery of Therapeutic Small Molecules

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