Myoblast Cell Interaction with Polydopamine Coated Liposomes

Westen, Rebecca van der; Hosta‐Rigau, Leticia; Sutherland, Duncan S.; Goldie, Kenneth N.; Alberício, Fernando; Postma, Almar; Städler, Brigitte

doi:10.1007/s13758-011-0008-4

Cited by 43 publications

(35 citation statements)

References 33 publications

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“…Importantly, the results showed a reduced cell viability of only ∼ 20% when TC was administered in solution in contrast to the ∼ 50% reduced cell viability when the same amount of drug ( ∼ 0.8 ng) was delivered to the cells from the surface, suggesting a more effective celldrug interaction in the latter case. Further, the PDA coating did not have a detrimental effect on the presence and activity of TC in agreement with our previous report, [ 41 ] despite the high abundant sites for modifi cation by polymerizing dopamine.…”

supporting

confidence: 92%

Surface‐Adhered Composite Poly(Vinyl Alcohol) Physical Hydrogels: Polymersome‐Aided Delivery of Therapeutic Small Molecules

Hosta‐Rigau

Jensen

Fjeldsø

et al. 2012

Adv Healthcare Materials

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tissue engineering scaffolds in order to allow for more complex tissue to be cultured. [ 7 ] SMDD can be employed in two different manners: either by releasing the cargo into the surrounding media, such as the blood-stream, or by delivering the therapeutic compound to the cells adhered to the surface. In the latter case, the creation of a polymer coating which promotes both cell adhesion and proliferation is of paramount importance. To date, the sequential deposition of interacting polymer layers to create polymer thin fi lms appears to stand out since it is a versatile, inexpensive yet effi cient technique to assemble advanced coatings. [ 8 ] Successful delivery of macromolecular cargo, e.g., oligonucleotides [ 9 ] or proteins, [ 10 ] from a surface has already been documented including the implementation in vivo. However, SMDD of small therapeutic compounds still remains a challenge, and its success is limited to specifi c examples such as the use of drug-polymer conjugates [ 11 ] or the immobilization within a carrier ( e.g. , liposomes, [ 12 ] micelles, [ 13 ] or cyclodextrins). [ 14 ] We recently reported the uptake of fl uorescent lipids by myoblast cells from a liposome-containing thin fi lm of PDA. [ 15 ] Similarly, bulk hydrogels loaded with drug-containing smaller components in the form of microparticles, [ 16 ] liposomes, [ 17 ] polymersomes, [ 18 ] or micelles, [ 19 ] are termed "composite hydrogels" and were considered to gain an enhanced control over the cargo retention and release. However, to the best of our knowledge, surface-adhered composite hydrogels and their utility in SMDD has never been considered and is presented herein for the fi rst time.As sub-compartments for PVA hydrogels, we chose polymersomes, [ 20 ] colloidal vessels which self-assemble from amphiphilic block copolymers with a well-documented Surface-mediated drug delivery (SMDD) is an approach which aims to administer therapeutics to adhering or suspension cells from implantable devices or from tissue engineering scaffolds. Herein is reported the proof of concept for polymersome-aided trapping of the cytotoxic hydrophobic compound thiocoraline in a physical poly(vinyl alcohol) (PVA) hydrogel matrix and the subsequent viability of myoblast cells cultured on this surfaceadhered composite hydrogel. The ABA triblock copolymer, poly( N -acryloyl morpholine)-block -poly(cholesteryl acrylate)-block -poly( N -acryloyl morpholine) (PNAM-b -PChA-b -PNAM), is synthesized, and the self-assembly of this polymer into polymersomes is shown. The polymersomes are loaded with the model cargo fl uorescein in order to visualize the entrapping of the small payload in this composite hydrogel. To render the PVA hydrogels cell adhesive, a poly(dopamine) (PDA) coating is successfully applied to the biointerface. Finally, to demonstrate the feasibility that active cargo can be entrapped and yield a cell response, the composite hydrogels are loaded with the cytotoxic depsipeptide thiocoraline and used in SMDD. The cargo activity is ascertained via monitori...

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confidence: 92%

Surface‐Adhered Composite Poly(Vinyl Alcohol) Physical Hydrogels: Polymersome‐Aided Delivery of Therapeutic Small Molecules

Hosta‐Rigau

Jensen

Fjeldsø

et al. 2012

Adv Healthcare Materials

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“…138,139 For example, polydopamine-coated liposomes led to higher myoblast viability and uptake efficiency compared to unmodified ones. 116 Lynge et al demonstrated that myoblasts adhered and proliferated on polydopamine-coated glass slides. 118 These cells were also shown to successfully internalize fluorescently labeled zwitterionic liposomes coated with polydopamine, with the highest fluorescence intensity concentrated near the nuclei.…”

Section: Applications In Tissue Engineeringmentioning

confidence: 99%

Materials from Mussel-Inspired Chemistry for Cell and Tissue Engineering Applications

et al. 2015

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Current advances in biomaterial fabrication techniques have broadened their application in different realms of biomedical engineering, spanning from drug delivery to tissue engineering. The success of biomaterials depends highly on the ability to modulate cell and tissue responses, including cell adhesion, as well as induction of repair and immune processes. Thus, most recent approaches in the field have concentrated on functionalizing biomaterials with different biomolecules intended to evoke cell- and tissue-specific reactions. Marine mussels produce mussel adhesive proteins (MAPs), which help them strongly attach to different surfaces, even under wet conditions in the ocean. Inspired by mussel adhesiveness, scientists discovered that dopamine undergoes self-polymerization at alkaline conditions. This reaction provides a universal coating for metals, polymers, and ceramics, regardless of their chemical and physical properties. Furthermore, this polymerized layer is enriched with catechol groups that enable immobilization of primary amine or thiol-based biomolecules via a simple dipping process. Herein, this review explores the versatile surface modification techniques that have recently been exploited in tissue engineering and summarizes polydopamine polymerization mechanisms, coating process parameters, and effects on substrate properties. A brief discussion of polydopamine-based reactions in the context of engineering various tissue types, including bone, blood vessels, cartilage, nerves, and muscle, is also provided.

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“…51 Westen et al reported on coating liposomes with PDA, which demonstrated their potential as drug delivery vehicle. 52 Melanin-like PDA nanoparticles were prepared by this bioinspired polymerization of dopamine, which had an excellent free-radical-scavenging property. 45 Because of the bioinspired nature of polydopamine, the bioapplication of PDA has attracted great attention.…”

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confidence: 99%

Mussel-Inspired Polydopamine: A Biocompatible and Ultrastable Coating for Nanoparticles in Vivo

et al. 2013

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Bioinspired polydopamine (PDA) has served as a universal coating to nanoparticles (NPs) for various biomedical applications. However, one remaining critical question is whether the PDA shell on NPs is stable in vivo. In this study, we modified gold nanoparticles (GNPs) with finely controlled PDA nanolayers to form uniform core/shell nanostructures (GNP@PDA). In vitro study showed that the PDA-coated GNPs had low cytotoxicity and could smoothly translocate to cancer cells. Transmission electron microscopy (TEM) analysis demonstrated that the PDA nanoshells were intact within cells after 24 h incubation. Notably, we found the GNP@PDA could partially escape from the endosomes/lysosomes to cytosol and locate close to the nucleus. Furthermore, we observed that the PDA-coated NPs have very different uptake behavior in two important organs of the liver and spleen: GNP@PDA in the liver were mainly uptaken by the Kupffer cells, while the GNP@PDA in the spleen were uptaken by a variety of cells. Importantly, we proved the PDA nanoshells were stable within cells of the liver and spleen for at least six weeks, and GNP@PDA did not show notable histological toxicity to main organs of mice in a long time. These results provided the direct evidence to support that the PDA surface modification can serve as an effective strategy to form ultrastable coatings on NPs in vivo, which can improve the intracellular delivery capacity and biocompatibility of NPs for biomedical application.

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Myoblast Cell Interaction with Polydopamine Coated Liposomes

Cited by 43 publications

References 33 publications

Surface‐Adhered Composite Poly(Vinyl Alcohol) Physical Hydrogels: Polymersome‐Aided Delivery of Therapeutic Small Molecules

Surface‐Adhered Composite Poly(Vinyl Alcohol) Physical Hydrogels: Polymersome‐Aided Delivery of Therapeutic Small Molecules

Materials from Mussel-Inspired Chemistry for Cell and Tissue Engineering Applications

Mussel-Inspired Polydopamine: A Biocompatible and Ultrastable Coating for Nanoparticles in Vivo

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