Electrochemically Driven Derivatisation-Detection of Cysteine

White, Paul; Lawrence, Nathan S.; Tsai, Yu Chen; Davis, James A.; Compton, Richard G.

doi:10.1007/s006040170033

Cited by 35 publications

(24 citation statements)

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“…The electrochemical oxidation of catechol in the presence of cysteine has been shown to lead to the deposition of a quinone-cysteine adduct at the electrode surface. Square wave voltammetric techniques allowed electrochemical processes due to the unreacted orthoquinone and the accumulated adduct species to be resolved such that the latter could be effected as a means of quantifying cysteine [95]. A second reaction process relying upon the reaction of various quinone structures with cysteine has also been detailed [26].…”

Section: Derivatization Detectionmentioning

confidence: 99%

Electrochemical Determination of Thiols: A Perspective

et al. 2002

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Monitoring thiol concentrations within physiological fluids has risen to considerable importance within the biomedical communities as our appreciation of the various roles that these compounds can perform has increased. Their utilization as biomarkers for numerous clinical conditions has prompted considerable interest in the development of analytical protocols that enable speedy measurements to be made. As such, a large number of procedures have arisen. This perspective highlights the contexts where physiological thiol measurements are required and describes the basis of many of the current electrochemical approaches to their determination. The collation of information from a broad range of sources covering the major electrochemical approaches (direct, indirect, and post column/capillary detection) has been achieved and the salient points of each technique critically appraised. The various characteristics and limitations of the systems are compared and contrasted with the analytical parameters (matrix, methodology, detection limit etc) from each major grouping tabulated to aid direct comparison.

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Section: Derivatization Detectionmentioning

confidence: 99%

Electrochemical Determination of Thiols: A Perspective

et al. 2002

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“…One common type of point-to-care sensors is that based on carbon screen-printed electrode [20][21][22][23]. It is best suited for applications that require high throughput testing, such as clinical testing [22][23][24]. To the best of our knowledge, there is no literature reporting thiol detection in saliva without the use of expensive instrumentation or elaborate separation technique.…”

Section: Introductionmentioning

confidence: 98%

“…Electrochemical method in the form of point-of-care sensors has its major advantages over the mentioned methodologies as measurements can be quick, easy, and cheap. One common type of point-to-care sensors is that based on carbon screen-printed electrode [20][21][22][23]. It is best suited for applications that require high throughput testing, such as clinical testing [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Selective electrochemical detection of thiol biomarkers in saliva using multiwalled carbon nanotube screen-printed electrodes

Lee

Compton

2015

Sensors and Actuators B: Chemical

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“…As 89.3% of human proteins and 17% of their tryptic peptides possess at least one cysteine [59], the proposed methodology was targeted toward cysteine mass tagging. Based on recent works on the electrochemical detection of sulfides [60,61], thiols [62], and cysteine [63,64] with quinone derivatives, we focused on the potential use of electrogenerated benzoquinones (coming from the oxidation of hydroquinones) able to react specifically with cysteine residues during electrospray mass spectrometric measurements. We present below a review of the different steps of the development of on-line electrochemical probes for cysteine mass tagging, from the proof of principle [65] to the elucidation of the tagging reaction mechanism [66], characterization of the microspray design influence on the tagging process [67], search for an optimal electrochemical probe [68], and final application to protein identification [69].…”

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confidence: 99%

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Roussel

Dayon

Lion

et al. 2004

J. Am. Soc. Mass Spectrom.

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We present herein a review of our work on the on-line electrochemical generation of mass tags toward cysteine residues in peptides and proteins. Taking advantage of the inherent electrochemical nature of electrospray generated from a microfabricated microspray emitter, selective probes for cysteine were developed and tested for on-line nonquantitative mass tagging of peptides and proteins. The nonquantitative aspect of the covalent tagging thus allows direct counting of free cysteines in the mass spectrum of a biomolecule through additional adduct peaks. Several substituted hydroquinones were investigated in terms of electrochemical properties, and their usefulness for on-line mass tagging during microspray experiments were assessed with L-cysteine, peptides, and intact proteins. Complementarily, numerical simulations were performed to properly understand the respective roles of mass transport, kinetics of electrochemical-chemical reactions, and design of the microspray emitter in the mass tagging overall efficiency. Finally, the on-line electrochemical tagging of cysteine residues was applied to the analysis of tryptic peptides of purified model proteins for protein identification through peptide mass fingerprinting. (J Am Soc Mass Spectrom 2004, 15, 1767-1779

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Electrochemically Driven Derivatisation-Detection of Cysteine

Cited by 35 publications

References 1 publication

Electrochemical Determination of Thiols: A Perspective

Electrochemical Determination of Thiols: A Perspective

Selective electrochemical detection of thiol biomarkers in saliva using multiwalled carbon nanotube screen-printed electrodes

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

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