Electrochemical Tau Protein Immunosensor Based on MnS/GO/PANI and Magnetite‐incorporated Gold Nanoparticles

Abstract

Herein, electrochemical tau protein immunosensor based on manganese sulfide nanoparticles/graphene oxide/polyaniline (MnS/GO/PANI) and magnetiteincorporated gold nanoparticles (AuNPs@Fe 3 O 4 ) was constructed. After the modification of the glassy carbon electrode (GCE) with MnS/GO/PANI, the immobilization and conjugation of anti-Tau capture antibody and antigen Tau protein were successfully completed on MnS/GO/ PANI/GCE, respectively. Then, the conjugation of anti-Tau secondary antibody was carried out to AuNPs@Fe 3 O 4 as signal amplification via amino-gold affinity and the eventual immunosensor was accomplished by the distinctive interactions of electrode platform and signal amplification. Final immunosensor demonstrated the quantification limit (LOQ) of 1.0 × 10 À 13 M and the detection limit (LOD) of 1.0 × 10 À 14 M.

Recent advances in the utilization of polyaniline in protein detection: a short review

“…Lately a tau protein immunosensor based on manganese sulphide (MS) graphene oxide (GO) and PANI (MnS/GO/PANI) with magnetite-incorporated gold nanoparticles immunosensor has been developed for the electrochemical detection of tau protein which exhibited superior selectivity, sensitivity, and environmental compatibility. 42 The sensor demonstrated the a detection limit of 1.0 × 10 −14 M which could be utilized for diagnosis of Alzheimers disease. Park et al 43 designed an aptasensor based on PANI and carbon nanotube (CNT) for detecting VEGF165 as a tumor marker with a limit of detection of 0.4 pg mL −1 and showed high selectivity in presence of other proteins.…”

Graphene quantum dots incorporated NiAl2O4 nanocomposite based molecularly imprinted electrochemical sensor for 5-hydroxymethyl furfural detection in coffee samples

“…Voltammetry is an electro-analytical method, based on current-voltage and concentration relations, which provides information about the analyte by measuring the current at the applied potential in conditions where the working electrode is polarized. 7,8 Thus, this method can provide important contributions in the eld of health and nutrition by identifying and eliminating the possible negative effects of HMF on the health of living organisms, especially humans.…”

CuO nanoleaf and β-cyclodextrin functionalized reduced graphene oxide: a highly selective and sensitive electrochemical sensor for the simultaneous detection of 2-chlorophenol and 2, 4-dichlorophenol

“…These carbon materials and their hybrid composites have been widely used in the study and development of a variety of novel biosensors and immunosensors. [21][22][23][24][25][26] From the literature survey, it is observed that functionalized graphene oxide, reduced graphene oxide, carbon nanotubes, and graphitic carbon nitride have also been used for the detection and determination of 2-CP or 2,4-DCP individually. Functionalization was performed mainly by incorporating metal, metal oxide nanoparticles, metal-organic frameworks, or cyclodextrins.…”