Electrochemical tagging of urate: developing new redox probes

Dutt, Jodi S. N.; Cardosi, Marco F.; Davis, James A.

doi:10.1039/b303399f

Cited by 30 publications

(15 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Uric acid is the final oxidation product of purine metabolism ( Yamamoto et al, 2005 ). Abnormality of the uric acid concentration is sensitive indicator of certain pathological states, including gout, hyperuricemia, renal failure, toxemia during pregnancy ( Dutt et al, 2003 ). Previous study indicated plasma uric acid increased significantly in patients with ESRD and fell after hemodialysis ( Shahbazian et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Rhubarb Protect Against Tubulointerstitial Fibrosis by Inhibiting TGF-β/Smad Pathway and Improving Abnormal Metabolome in Chronic Kidney Disease

Zhang¹,

Li²,

Liú³

et al. 2018

Front. Pharmacol.

View full text Add to dashboard Cite

Tubulointerstitial fibrosis is the final common pathway for all kidney diseases leading to chronic kidney disease (CKD). TGF-β/Smad signaling pathway plays a key role in renal fibrosis. Previous studies have revealed that rhubarb extracts attenuated the increase of transforming growth factor-β 1 (TGF-β1) in CKD rats. To gain an in-depth insight into the mechanism of the anti-fibrotic activities of the rhubarb extracts, we investigated the influence of rhubarb extracts on TGF-β/Smad signaling pathway and the influence on metabolome in a rat model of CKD with adenine-induced chronic tubulointerstitial nephropathy. Male Sprague-Dawley rats were divided into four groups, including control, CKD, CKD + petroleum ether extract, CKD + ethyl acetate extract, and CKD + n-butanol extract groups. Kidneys harvested on the week three were evaluated for renal fibrosis, the expression of proteins in TGF-β/Smad signaling pathway and metabolomic study. We found rhubarb extracts suppressed TGF-β/Smad3-mediated renal fibrosis by reducing the TGF-β1, transforming growth factor-β receptor I (TGF-β RI), transforming growth factor-β receptor II (TGF-β RII), Smad2, p-Smad2, Smad3, p-Smad3, and Smad4, meanwhile increased Smad7. In addition, rhubarb extracts mitigated renal injury and dysfunction, and either fully or partially reversed the abnormalities of tissue metabolites. Thus, rebalancing the disorder of TGF-β/Smad signaling and metabolic dysfunction by treatment with rhubarb extracts may represent as an effective therapy for CKD associated with fibrosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Rhubarb Protect Against Tubulointerstitial Fibrosis by Inhibiting TGF-β/Smad Pathway and Improving Abnormal Metabolome in Chronic Kidney Disease

Zhang¹,

Li²,

Liú³

et al. 2018

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…XA and HX are intermediates, and UA is the final oxidation product of the purine metabolism of the cells of humans, animals, and plants [3][4][5]. Their concentration levels are sensitive indicators of certain diseases, including perinatal asphyxia, cerebral ischemia, hyperuricemia, gout CONTACT Junwei Yang yangjw@sdju.edu.cn [6][7][8], and positive effect on cardiovascular system [9][10][11][12][13]. Therefore, the accurate measurement of HX, XA, and UA is very important in food, biochemical, and clinical diagnosis [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Density functional theory study of interaction of graphene with hypoxanthine, xanthine, and uric acid

2016

View full text Add to dashboard Cite

Hypoxanthine (HX), xanthine (XA), and uric acid (UA) are important molecules in human beings. However, the physics underlying the interaction between these molecules and carbon nanomaterials is still unclear. In this study, we theoretically anlaysed the interaction of the graphene with HX, XA, and UA via the LDA, Perdew-Burke-Ernzerhof, and a second version of van der Waals (vdW)-DF2 exchange-correlation functionals of density functional theory. It was observed that these molecules could be adsorbed on the surface of the graphene steadily. The adsorption energies of these molecules on the graphene for the same site in vdW-DF2 functional show the following ordering: UA > XA > HX, the adsorption distances the following ordering: UA < XA < HX. The mechanism of this stable adsorption was revealed to be π -π and O-π interactions between these molecules and the graphene. Simulated scanning tunnelling microscopy (STM) images of HX, XA, and UA on the surface of the graphene layer as application, HX can be distinguished from XA and UA via the STM technique as a result of the interaction between the graphene and these molecules. The findings of this study provide a theoretical reference in the development and design of highly accurate biodevices and biosensors.

show abstract

“…Abnormalities of the metabolites concentrations are sensitive indicators of certain pathologic states, including gout, xanthinuria, hyperuricemia, renal failure, toxaemia during pregnancy, etc. [22][23][24]. Since Hx and X are the precursors of UA and important intermediates in purine biosynthesis, simultaneous detection of these two compounds have become vital in food, biochemical and clinical diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Ru(DMSO)4Cl2 nano-aggregated Nafion membrane modified electrode for simultaneous electrochemical detection of hypoxanthine, xanthine and uric acid

Kumar

Swetha

2010

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

Electrochemical tagging of urate: developing new redox probes

Abstract: The electro-oxidation of uric acid in the presence of nucleophilic species is shown to produce conjugates that can aid our understanding of antioxidant interactions and also provide opportunities for advancing electroanalytical detection strategies involving purine species.

Cited by 30 publications

References 9 publications

Rhubarb Protect Against Tubulointerstitial Fibrosis by Inhibiting TGF-β/Smad Pathway and Improving Abnormal Metabolome in Chronic Kidney Disease

Rhubarb Protect Against Tubulointerstitial Fibrosis by Inhibiting TGF-β/Smad Pathway and Improving Abnormal Metabolome in Chronic Kidney Disease

Density functional theory study of interaction of graphene with hypoxanthine, xanthine, and uric acid

Ru(DMSO)4Cl2 nano-aggregated Nafion membrane modified electrode for simultaneous electrochemical detection of hypoxanthine, xanthine and uric acid

Contact Info

Product

Resources

About