Electrochemical studies of homogeneous self-assembled monolayers versus mixed self-assembled monolayers on gold electrode for “label free” detection of heart fatty acid binding protein

Stan, Dana; Mihailescu, Carmen-Marinela; Iosub, Rodica; Moldovan, Carmen; Savin, Mihaela; Ion, Baciu

doi:10.1016/j.tsf.2012.11.028

Cited by 26 publications

(12 citation statements)

References 27 publications

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“…The decrease of DTPA on the electrode is not a serious issue. In case of 1 % HT, the presence of about 5 × 10 14 DTPA molecules in the binary SAMs may be enough to bind with proteins, and the resulting space due to HT can afford an useful reaction site where the immunoreaction efficiently takes place [21,24]. It is not surprising that the resulting ratio of DTPA to HT molecules in the binary SAMs is significantly different from that of the preparation solution [20].…”

Section: Resultsmentioning

confidence: 99%

“…Binary SAMs formed from two components of thiols and/or disulfides are noteworthy for easily giving higher functionality in comparison with mono SAMs. Recently, several researchers have tried to use binary SAMs that create some space for bulky enzymes or antibodies to efficiently work [21], and for electron transfer [22], in addition to immobilization of proteins [23,24]. As far as we are aware, the application of the binary SAMs to ECL sensors has not been found in any research as yet.…”

Section: Introductionmentioning

confidence: 99%

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Detection of influenza virus by a biosensor based on the method combining electrochemiluminescence on binary SAMs modified Au electrode with an immunoliposome encapsulating Ru (II) complex

et al. 2016

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Recently, point of care testing (POCT) used for diagnosis of influenza infection has a problem showing false negative diagnosis because of the low sensitivity. We would like to report detection of influenza virus A (H1N1) by an immunosensor based on electrochemiluminescence (ECL) that uses an immunoliposome encapsulating tris(2,2'-bipyridyl)ruthenium(II) complex. By using the sensor, we could detect the virus that competed with hemagglutinin (HA) peptide immobilized on self-assembled monolayers (SAMs) in immunoreaction of the antibody bound on the surface of liposome. The HA peptide was 19 mer (TGLRNGITNKVNSVIEKAA). We demonstrated great improvement of sensitivity and accuracy by introducing binary SAMs instead of mono SAMs. The binary SAMs was prepared from 3,3'-dithiodipropionic acid and 1-hexanethiol. Use of the binary SAMs enabled to increase the SAMs coverage on Au electrode; the fact was confirmed by observation of the cathodic desorption currents. By using such an electrode, first the detection method of BSA was optimized to lower ECL background signal. Then we applied the method to the detection of influenza virus. We could successfully detect the virus with higher sensitivity compared with that by POCT and ELISA. The detection range was from a concentration of 2.7 × 10(2) to 2.7 × 10(3) PFU/mL.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of influenza virus by a biosensor based on the method combining electrochemiluminescence on binary SAMs modified Au electrode with an immunoliposome encapsulating Ru (II) complex

et al. 2016

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“…The H-FABP is another cardiac biomarker which can be electrochemically detected for AMI diagnosis. H-FABP is a protein smaller than myoglobin and an abundant protein in cytosol of cardiomyocytes [66]. H-FABP is considered as an early maker for diagnosis of AMI because, due to its small size and solubility, it is more quickly released in the into blood circulation in the early stage of cardiac injury than structurally bound biomarkers [67].…”

Section: Other Cardiac Biomarkers For Amimentioning

confidence: 99%

Nanomaterials‐based Electrochemical Sensing of Cardiac Biomarkers for Acute Myocardial Infarction: Recent Progress

Nsabimana

Yuan

et al. 2018

Electroanalysis

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The development of the methods for early and accurate diagnosis of acute myocardial infarction are needed to facilitate immediate treatment of patients. One of the ways to achieve that is the detection of cardiac biomarkers for myocardial infarction, such as thrombin, cardiac troponins (I and T), myoglobin, etc. Nanotechnology has played an important role in the development of sensitive and efficient electrochemical sensors for cardiac biomarkers. In this review, we discuss recent progress on nanomaterial‐based electrochemical sensing of various cardiac biomarkers for acute myocardial infarction.

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“…This indicates that there may be an abundance of free amine groups on the substrate surface, which are not involved in complex formation with TNT. However, AHT forms dense monolayer on the surface and therefore TNT molecules may experience some steric hindrance to reach the amine groups of the AHT layer [55]. Therefore, optimizing the surface distribution of AHT is vital for the effective complex formation between AHT and TNT.…”

Section: Electrochemical and Xps Characterization Of Ca And Aht Modifmentioning

confidence: 99%

Molecular recognition of 2,4,6-trinitrotoluene by 6-aminohexanethiol and surface-enhanced Raman scattering sensor

Jamil

Sivanesan

Izake

et al. 2015

Sensors and Actuators B: Chemical

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TNT) sensor 6-Aminohexanethiol (AHT) and mixed monolayer Surface-enhanced Raman spectroscopy (SERS) Electrochemical desorption and X-ray photoelectron spectroscopy (XPS) Analysis of explosives in soil a b s t r a c t 2,4,6-Trinitrotoluene (TNT) is one of the most commonly used nitro aromatic explosives in landmine, military and mining industry. This article demonstrates rapid and selective identification of TNT by surface-enhanced Raman spectroscopy (SERS) using 6-aminohexanethiol (AHT) as a new recognition molecule. First, Meisenheimer complex formation between AHT and TNT is confirmed by the development of pink color and appearance of new band around 500 nm in UV-vis spectrum. Solution Raman spectroscopy study also supported the AHT:TNT complex formation by demonstrating changes in the vibrational stretching of AHT molecule between 2800 and 3000 cm −1 . For surface enhanced Raman spectroscopy analysis, a self-assembled monolayer (SAM) of AHT is formed over the gold nanostructure (AuNS) SERS substrate in order to selectively capture TNT onto the surface. Electrochemical desorption and Xray photoelectron studies are performed over AHT SAM modified surface to examine the presence of free amine groups with appropriate orientation for complex formation. Further, AHT and butanethiol (BT) mixed monolayer system is explored to improve the AHT:TNT complex formation efficiency. Using a 9:1 AHT:BT mixed monolayer, a very low detection limit (LOD) of 100 fM TNT was realized. The new method delivers high selectivity towards TNT over 2,4 DNT and picric acid. Finally, real sample analysis is demonstrated by the extraction and SERS detection of 302 pM of TNT from spiked. Crown

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Electrochemical studies of homogeneous self-assembled monolayers versus mixed self-assembled monolayers on gold electrode for “label free” detection of heart fatty acid binding protein

Cited by 26 publications

References 27 publications

Detection of influenza virus by a biosensor based on the method combining electrochemiluminescence on binary SAMs modified Au electrode with an immunoliposome encapsulating Ru (II) complex

Detection of influenza virus by a biosensor based on the method combining electrochemiluminescence on binary SAMs modified Au electrode with an immunoliposome encapsulating Ru (II) complex

Nanomaterials‐based Electrochemical Sensing of Cardiac Biomarkers for Acute Myocardial Infarction: Recent Progress

Molecular recognition of 2,4,6-trinitrotoluene by 6-aminohexanethiol and surface-enhanced Raman scattering sensor

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