Electrochemical sensory detection of<i>Sus scrofa</i>mtDNA for food adulteration using hybrid ferrocenylnaphthalene diimide intercalator as a hybridization indicator

Kusnin, Norzila; Yusof, Nor Azah; Abdullah, Jaafar; Sabri, Suriana; Mohammad, Faruq; Mustafa, Shuhaimi; Mutalib, Nurul Asyikeen Ab; Sato, Shinobu; Takenaka, Shigeori; Parmin, N. A.; Al‐Lohedan, Hamad A.

doi:10.1039/d0ra03585h

Cited by 15 publications

(15 citation statements)

References 40 publications

(30 reference statements)

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“…These findings imply that electrode modification with AuNPs/ Gr enhances electrocatalytic activity. Many researchers have re-ported a similar shape of the Nyquist plot when different electrodes are modified [57][58][59].…”

Section: Electrochemical Characterisation Of Screen-printed Carbon El...mentioning

confidence: 91%

Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite

Hashem¹,

Hossain²,

Marlinda³

et al. 2022

Beilstein J. Nanotechnol.

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The Southeast Asian box turtle, Cuora amboinensis, is an ecologically important endangered species which needs an onsite monitoring device to protect it from extinction. An electrochemical DNA biosensor was developed to detect the C. amboinensis mitochondrial cytochrome b gene based on an in silico designed probe using bioinformatics tools, and it was also validated in wet-lab experiments. As a detection platform, a screen-printed carbon electrode (SPCE) enhanced with a nanocomposite containing gold nanoparticles and graphene was used. The morphology of the nanoparticles was analysed by field-emission scanning electron microscopy and structural characteristics were analysed by using energy-dispersive X-ray, UV–vis, and Fourier-transform infrared spectroscopy. The electrochemical characteristics of the modified electrodes were studied by cyclic voltammetry, differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy. The thiol-modified synthetic DNA probe was immobilised on modified SPCEs to facilitate hybridisation with the reverse complementary DNA. The turtle DNA was distinguished based on hybridisation-induced electrochemical change in the presence of methylene blue compared to their mismatches, noncomplementary, and nontarget species DNA measured by DPV. The developed biosensor exhibited a selective response towards reverse complementary DNAs and was able to discriminate turtles from other species. The modified electrode displayed good linearity for reverse complementary DNAs in the range of 1 × 10−11–5 × 10−6 M with a limit of detection of 0.85 × 10−12 M. This indicates that the proposed biosensor has the potential to be applied for the detection of real turtle species.

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Section: Electrochemical Characterisation Of Screen-printed Carbon El...mentioning

confidence: 91%

Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite

Hashem¹,

Hossain²,

Marlinda³

et al. 2022

Beilstein J. Nanotechnol.

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“…However, most earlier MB research focused on synthetic DNA sequences or puried samples, and the use of MB for electrochemical detection based on large DNA sequences from real samples is currently still limited. Most recent works related to electrochemical DNA biosensors have focused on studying miniaturized electrochemical sensor devices, 22 amplication signals, 23,24 biorecognition probes with new detection mechanisms, 25 and the optimization of immobilization and hybridization conditions. 26 However, far too little attention has been focused on the preparation of genomic DNA samples for electrochemical detection.…”

Section: Introductionmentioning

confidence: 99%

Strategies for the preparation of non-amplified and amplified genomic dengue gene samples for electrochemical DNA biosensing applications

et al. 2022

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“…18 Analytical chemistry guarantees food safety and quality through the development of reliable sensitive and selective analytical methods to detect and quantify food adulterants and food ingredients. [19][20][21][22] Analytical reports provide authorities, food suppliers and consumers with the evidence necessary to build condence in food authenticity, safety and quality. 23 Analyzing pharmaceutical and food products is a highly multivariate process, as many factors (parameters) may be involved during the analysis.…”

Section: Introductionmentioning

confidence: 99%

Experimentally designed chemometric models for the assay of toxic adulterants in turmeric powder

et al. 2022

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Electrochemical sensory detection ofSus scrofamtDNA for food adulteration using hybrid ferrocenylnaphthalene diimide intercalator as a hybridization indicator

Abstract: An electrochemical DNA biosensor was developed based on the fabrication of silicon nanowires/platinum nanoparticles on a screen-printed carbon electrode for the detection of Sus scrofa mitochondrial DNA in food.

Cited by 15 publications

References 40 publications

Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite

Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite

Strategies for the preparation of non-amplified and amplified genomic dengue gene samples for electrochemical DNA biosensing applications

Experimentally designed chemometric models for the assay of toxic adulterants in turmeric powder

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