Electrochemical sensing system employing fructosamine 6‐kinase enables glycated albumin measurement requiring no proteolytic digestion

Kameya, Miho; Tsugawa, Wakako; Yamada-Tajima, Mayumi; Hatada, Mika; Suzuki, Keita; Sakaguchi-Mikami, Akane; Ferri, Stefano; Klonoff, David C.; Sode, Koji

doi:10.1002/biot.201500442

Cited by 21 publications

(8 citation statements)

References 47 publications

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“…[62] Kameya et al reported an enzyme-based GA biosensor using SPCE which can detect higher concentrations of GA, partially falling in the clinical relevant range, but not achieving measurement of the full GA clinical range. [68]…”

Section: Chronoamperometric Deposition Of Au Nps Is Done Bymentioning

confidence: 99%

When Less Gold is More: Selective Attomolar Biosensing at the Nanoscale

Dastidar

Murugappan

Damry

et al. 2022

Adv Funct Materials

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The development of biosensors has become increasingly important to tackle the spread of potentially pandemic pathogens and for the decentralization of healthcare services. Despite progress, achieving selective detection of ultralow concentrations of biomarkers in complex fluids with point‐of‐care devices is a standing challenge. Here, an efficient material platform for the sensing of biomarkers down to attomolar concentrations with excellent selectivity and a tuneable linear response range is reported. It is demonstrated that by decreasing the Au nanoislands on an elsewhere passivated surface, it is possible to more than double their electrochemical response to low biomarker concentrations via a gate‐type mechanism. As an exemplary application, the first sensing of glycated albumin, a key biomarker for diabetes management, in clinically relevant levels by conjugation of a selective DNA aptamer to the Au nanoisland surface is showcased. With a limit of detection of 0.55 × 10−18 m, the excellent selectivity of this platform against nonspecific biomolecules is validated. This platform shows exceptional sensitivity and selectivity in mouse serum, further highlighting its potential for clinical utility. The versatility of this platform to detect other biomarkers, like miRNAs, is also showcased. These findings provide a flexible material platform for the scalable and low‐cost engineering of future point‐of‐care biosensors.

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Section: Chronoamperometric Deposition Of Au Nps Is Done Bymentioning

confidence: 99%

When Less Gold is More: Selective Attomolar Biosensing at the Nanoscale

Dastidar

Murugappan

Damry

et al. 2022

Adv Funct Materials

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“…To overcome this issue, procedures that require no pre-digestion have been investigated. Kameya et al [ 100 ] exploited an enzyme retrieved from E. coli , fructosamine 6-kinase (FN6K) that can interact with intact GA; however, as pointed out by the authors, the reactions involved in this design require several additional steps and reagents (such as enzymes, co-substrates, buffers, mediators) that increase the complexity of the manufacturing and may lead to errors in the measurements. Table 3 compares the technologies presented.…”

Section: Glycated Albumin Analysismentioning

confidence: 99%

Analytical Challenges in Diabetes Management: Towards Glycated Albumin Point-of-Care Detection

et al. 2022

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Diabetes mellitus is a worldwide-spread chronic metabolic disease that occurs when the pancreas fails to produce enough insulin levels or when the body fails to effectively use the secreted pancreatic insulin, eventually resulting in hyperglycemia. Systematic glycemic control is the only procedure at our disposal to prevent diabetes long-term complications such as cardiovascular disorders, kidney diseases, nephropathy, neuropathy, and retinopathy. Glycated albumin (GA) has recently gained more and more attention as a control biomarker thanks to its shorter lifespan and wider reliability compared to glycated hemoglobin (HbA1c), currently the “gold standard” for diabetes screening and monitoring in clinics. Various techniques such as ion exchange, liquid or affinity-based chromatography and immunoassay can be employed to accurately measure GA levels in serum samples; nevertheless, due to the cost of the lab equipment and complexity of the procedures, these methods are not commonly available at clinical sites and are not suitable to home monitoring. The present review describes the most up-to-date advances in the field of glycemic control biomarkers, exploring in particular the GA with a special focus on the recent experimental analysis techniques, using enzymatic and affinity methods. Finally, analysis steps and fundamental reading technologies are integrated into a processing pipeline, paving the way for future point-of-care testing (POCT). In this view, we highlight how this setup might be employed outside a laboratory environment to reduce the time from measurement to clinical decision, and to provide diabetic patients with a brand-new set of tools for glycemic self-monitoring.

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“…Several studies about the electrochemical biosensor for GA measurement have been reported using enzymes, antibody, aptamer or peptide as the biorecognition molecules and based on amperometry, electrochemiluminescence (ECL), impedance, voltammetry or field effect transistor (FET) as sensing principles [7,[16][17][18][19][20][21][22][23]. These studied are summarized in Table 1.…”

Section: Measurement Of Fructosyl Lysine and Ga With An Ide Enzyme Sementioning

confidence: 99%

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

et al. 2021

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Glycated albumin (GA) is an important glycemic control marker for diabetes mellitus. This study aimed to develop a highly sensitive disposable enzyme sensor strip for GA measurement by using an interdigitated electrode (IDE) as an electrode platform. The superior characteristics of IDE were demonstrated using one microelectrode of the IDE pair as the working electrode (WE) and the other as the counter electrode, and by measuring ferrocyanide/ferricyanide redox couple. The oxidation current was immediately reached at the steady state when the oxidation potential was applied to the WE. Then, an IDE enzyme sensor strip for GA measurement was prepared. The measurement of fructosyl lysine, the protease digestion product of GA, exhibited a high, steady current immediately after potential application, revealing the highly reproducible measurement. The sensitivity (2.8 nA µM−1) and the limit of detection (1.2 µM) obtained with IDE enzyme sensor strip were superior compared with our previously reported sensor using screen printed electrode. Two GA samples, 15 or 30% GA, corresponding to healthy and diabetic levels, respectively, were measured after protease digestion with high resolution. This study demonstrated that the application of an IDE will realize the development of highly sensitive disposable-type amperometric enzyme sensors with high reproducibility.

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Electrochemical sensing system employing fructosamine 6‐kinase enables glycated albumin measurement requiring no proteolytic digestion

Cited by 21 publications

References 47 publications

When Less Gold is More: Selective Attomolar Biosensing at the Nanoscale

When Less Gold is More: Selective Attomolar Biosensing at the Nanoscale

Analytical Challenges in Diabetes Management: Towards Glycated Albumin Point-of-Care Detection

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

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