Electrochemical quartz crystal impedance study on immobilization of glucose oxidase in a polymer grown from dopamine oxidation at an Au electrode for glucose sensing

Li, Mingrui; Deng, Chunyan; Xie, Qingji; Yang, Yang; Yao, Shouzhuo

doi:10.1016/j.electacta.2006.02.023

Cited by 35 publications

(49 citation statements)

References 43 publications

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“…4s caption), leading to a decrease in the deposited amount of PFDO at the same time scale, which virtually resulted in a decrease of the response current of the PFDO-Hb/Au electrode. Similar phenomena were also reported in the case for PFDO as the GOD-immobilization matrix [18]. Although the exact mechanism for this phenomenon is not very clear at present, the increased electrostatic interaction of Hb (pI ¼ 7.4) with DA (pK a ¼ 10.6 [25]) and the oligomers generated during the oxidation of DA in the polymerization buffer of pH 9.18, as well as the enhanced surface-adsorption capacity of Hb at an increased concentration, may take effect.…”

supporting

confidence: 76%

“…We have also examined the effect of concentration of Hb on the response of the constructed electrode in a detection solution of pH 5.29 phosphate buffer, as depicted in Figure 4, where 20.0 mM phosphate buffer and 30.0 mM DA were always involved in the polymerization bath [18]. As is seen, Di ss increased with the increase in Hb concentration up to 2.00 mg mL…”

mentioning

confidence: 91%

“…A polymerization mechanism involving complicated ECECEE pathways was reported [16], and the polymer film from dopamine oxidation (PFDO), which consists of an uniformly distributed submicrometer polymer particles on an Au electrode surface [17], has been found to be of cationic permselectivity and developed in this lab as a very good matrix to immobilize antihuman immunoglobulin G and glucose oxidase (GOD) [17,18], which exist as an anionic protein of large molecular weight in the neutral phosphate buffer.…”

Section: Introductionmentioning

confidence: 98%

“…We reported previously that the PFDO is a good substrate to immobilize more glucose oxidase (GOD) for the construction of a glucose enzyme electrode with detection sensitivity higher than the matrices of poly(N-methylpyrrole) and poly(o-aminophenol) [18]. In this work for Hb immobilization, we also compared the PFDO substrate with two common polymers, polypyrrole (PPY) and poly(ophenylenediamine) (PoPD).…”

mentioning

confidence: 98%

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An Amperometric Hydrogen Peroxide Biosensor Based on a Hemoglobin‐Immobilized Dopamine‐Oxidation Polymer/Prussian Blue/Au Electrode

Deng

Chen

et al. 2006

Electroanalysis

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A novel method to immobilize hemoglobin (Hb) in a polymer grown from dopamine (DA) oxidation was proposed. The growth of the polymeric films during DA oxidation at the Prussian blue (PB) modified Au electrode in weak alkaline phosphate buffer (pH 9.18) and the immobilization of Hb into the polymeric films during their growth were traced by the electrochemical quartz crystal impedance analysis (EQCIA) method. A hydrogen peroxide (H 2 O 2 ) biosensor was thus constructed, and effects of experimental parameters on the sensor performance, including the applied potential, solution pH and electroactive interferents, were examined. At an optimal potential of À 0.25 V vs. SCE, the current response of the biosensor in the selected phosphate buffer (pH 5.29) was linear with the concentration of H 2 O 2 from 0.01 to 4.5 mM, with a lower limit of detection of 0.5 mM (S/N ¼ 3), short response time (within 10 s) and good anti-interferent ability. The Michaelis constant (K m app ) was estimated to be 3.80 mM. Compared with the separate film of PB or Hb, the composite film of Hb and PB exhibited a higher catalytic activity toward the reduction of H 2 O 2 , as a result of the additive effect of the chemical and biological catalyses.

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confidence: 76%

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confidence: 91%

Section: Introductionmentioning

confidence: 98%

mentioning

confidence: 98%

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An Amperometric Hydrogen Peroxide Biosensor Based on a Hemoglobin‐Immobilized Dopamine‐Oxidation Polymer/Prussian Blue/Au Electrode

Deng

Chen

et al. 2006

Electroanalysis

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“…The (K M ) of the system in this work was found to be 2.4 mM, implying that the Celestine blue/MWCNTs modified glassy carbon electrode exhibits a higher affinity for glucose. The value of K M for GOx in this work is much smaller than that obtained at glucose biosensors based on sol-gel organic -inorganic hybrid material, 22 mM [58], GOx immobilized at chitosan and gold nanoparticles 10.5 mM [59], GOx entrapped in polypyrrole film, 19 mM [60], GOx immobilized in dopamine polymer film 9.6 [61], GOX immobilized in nafion film, 14.91 mM [62],GOx immobilized on TiO 2 film, 6.08 mM [63] , and comparable with GOx immobilized onto nickel oxide nanoparticles, 2.7 mM [64] and GOx at edge plane of highly oriented pyrolytic graphite electrode, 2.52 mM [65].…”

Section: Amperometric Determination Of Glucose At Celestine Blue/mwcnmentioning

confidence: 98%

Fabrication of Glucose Biosensor Based on Encapsulation of Glucose‐Oxidase on Sol‐Gel Composite at the Surface of Glassy Carbon Electrode Modified with Carbon Nanotubes and Celestine Blue

2008

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A simple procedure was developed to prepare a glassy carbon electrode modified with multi walled carbon nanotubes (MWCNTs) and Celestin blue. Cyclic voltammograms of the modified electrode show stable and a well defined redox couple with surface confined characteristic at wide pH range (2 -12). The formal potential of redox couple (E') shifts linearly toward the negative direction with increasing solution pH. The surface coverage of Celestine blue immobilized on CNTs glassy carbon electrode was approximately 1.95 Â 10 À10 mol cm À2 . The charge transfer coefficient (a) and heterogeneous electron transfer rate constants (k s ) for GC/MWCNTs/Celestine blue were 0.43 and 1.26 s À1, respectively. The modified electrode show strong catalytic effect for reduction of hydrogen peroxide and oxygen at reduced overpotential. The glucose biosensor was fabricated by covering a thin film of sol-gel composite containing glucose oxides (GOx) on the surface of Celestine blue /MWCNTs modified GC electrode. The biosensor can be used successfully for selective detection of glucose based on the decreasing of cathodic peak current of oxygen. The detection limit, sensitivity and liner calibration rang were 0.3 mM, 18.3 mA/mM and 10 mM -6.0 mM, respectively. The accuracy of the biosensor for glucose detection was evaluated by detection of glucose in a serum sample, using standard addition protocol. In addition biosensor can reach 90% of steady currents in about 3.0 sec and interference effect of the electroactive existing species (ascorbic acid -uric acid and acetaminophen) was eliminated. Furthermore, the apparent Michaelis -Menten constant 2.4 mM, of GOx on the nano composite exhibits excellent bioelectrocatalytic activity of immobilized enzyme toward glucose oxidation. Excellent electrochemical reversibility of redox couple, high stability, technically simple and possibility of preparation at short period of time are of great advantages of this procedure for modification of glucose biosensor.

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One‐Pot Preparation of Polymer–Enzyme–Metallic Nanoparticle Composite Films for High‐Performance Biosensing of Glucose and Galactose

Xie

et al. 2009

Adv Funct Materials

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139

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New polymer–enzyme–metallic nanoparticle composite films with a high‐load and a high‐activity of immobilized enzymes and obvious electrocatalysis/nano‐enhancement effects for biosensing of glucose and galactose are designed and prepared by a one‐pot chemical pre‐synthesis/electropolymerization (CPSE) protocol. Dopamine (DA) as a reductant and a monomer, glucose oxidase (GOx) or galactose oxidase (GaOx) as the enzyme, and HAuCl4 or H2PtCl6 as an oxidant to trigger DA polymerization and the source of metallic nanoparticles, are mixed to yield polymeric bionanocomposites (PBNCs), which are then anchored on the electrode by electropolymerization of the remaining DA monomer. The prepared PBNC material has good biocompatibility, a highly uniform dispersion of the nanoparticles with a narrow size distribution, and high load/activity of the immobilized enzymes, as verified by transmission/scanning electron microscopy and electrochemical quartz crystal microbalance. The thus‐prepared enzyme electrodes show a largely improved amperometric biosensing performance, e.g., a very high detection sensitivity (99 or 129 µA cm−2 mM−1 for glucose for Pt PBNCs on bare or platinized Au), a sub‐micromolar limit of detection for glucose, and an excellent durability, in comparison with those based on conventional procedures. Also, the PBNC‐based enzyme electrodes work well in the second‐generation biosensing mode. The proposed one‐pot CPSE protocol may be extended to the preparation of many other functionalized PBNCs for wide applications.

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Electrochemical quartz crystal impedance study on immobilization of glucose oxidase in a polymer grown from dopamine oxidation at an Au electrode for glucose sensing

Cited by 35 publications

References 43 publications

An Amperometric Hydrogen Peroxide Biosensor Based on a Hemoglobin‐Immobilized Dopamine‐Oxidation Polymer/Prussian Blue/Au Electrode

An Amperometric Hydrogen Peroxide Biosensor Based on a Hemoglobin‐Immobilized Dopamine‐Oxidation Polymer/Prussian Blue/Au Electrode

Fabrication of Glucose Biosensor Based on Encapsulation of Glucose‐Oxidase on Sol‐Gel Composite at the Surface of Glassy Carbon Electrode Modified with Carbon Nanotubes and Celestine Blue

One‐Pot Preparation of Polymer–Enzyme–Metallic Nanoparticle Composite Films for High‐Performance Biosensing of Glucose and Galactose

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