One‐Pot Preparation of Polymer–Enzyme–Metallic Nanoparticle Composite Films for High‐Performance Biosensing of Glucose and Galactose

Fu, Yingchun; Li, Penghao; Xie, Qingji; Xu, Xiahong; Lei, Lihong; Chen, Chao; Zou, Can; Deng, Wei; Yao, Shouzhuo

doi:10.1002/adfm.200801576

Cited by 139 publications

(79 citation statements)

References 52 publications

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“…16 In addition to its fascinating adhesive properties, dopamine also possesses a remarkable reducing character, which can be used as an eco-friendly and effective reducing agent to fabricate polymer nanocomposites. 19 In Asia, the sacred lotus (Nelumbo nucifera) has been a symbol of purity for over 2000 years. Water droplets can roll freely in all directions and then pick up dirt particles on lotus leaves, resulting in the so-called self-cleaning effect or lotus effect (Fig.…”

Section: Introductionmentioning

confidence: 99%

Fusion of nacre, mussel, and lotus leaf: bio-inspired graphene composite paper with multifunctional integration

et al. 2013

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Multifunctional integration is an inherent characteristic for biological materials with multiscale structures. Learning from nature is an effective approach for scientists and engineers to construct multifunctional materials. In nature, mollusks (abalone), mussels, and the lotus have evolved different and optimized solutions to survive. Here, bio-inspired multifunctional graphene composite paper was fabricated in situ through the fusion of the different biological solutions from nacre (brick-and-mortar structure), mussel adhesive protein (adhesive property and reducing character), and the lotus leaf (self-cleaning effect). Owing to the special properties (self-polymerization, reduction, and adhesion), dopamine could be simultaneously used as a reducing agent for graphene oxide and as an adhesive, similar to the mortar in nacre, to crosslink the adjacent graphene. The resultant nacre-like graphene paper exhibited stable superhydrophobicity, self-cleaning, anticorrosion, and remarkable mechanical properties underwater. Multifunctional integration is an inherent characteristic for biological materials with multiscale structures.Learning from nature is an effective approach for scientists and engineers to construct multifunctional materials. In nature, mollusks (abalone), mussels, and the lotus have evolved different and optimized solutions to survive. Here, bio-inspired multifunctional graphene composite paper was fabricated in situ through the fusion of the different biological solutions from nacre (brick-and-mortar structure), mussel adhesive protein (adhesive property and reducing character), and the lotus leaf (self-cleaning effect).Owing to the special properties (self-polymerization, reduction, and adhesion), dopamine could be simultaneously used as a reducing agent for graphene oxide and as an adhesive, similar to the mortar in nacre, to crosslink the adjacent graphene. The resultant nacre-like graphene paper exhibited stable superhydrophobicity, self-cleaning, anti-corrosion, and remarkable mechanical properties underwater.

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Section: Introductionmentioning

confidence: 99%

Fusion of nacre, mussel, and lotus leaf: bio-inspired graphene composite paper with multifunctional integration

et al. 2013

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“…1C, only a small number of Au NPs were observed on the PDA-coated PDMS surface. As is well known, DA is a reductant and a monomer, and HAuCl 4 or HPtCl 6 can be utilized as an oxidizing reagent to trigger DA polymerization and the source of metallic nanoparticles [29]. After filling the mixture of DA and HAuCl 4 (molar concentration ratio of 7.5:1) into the PDMS microchannel and reacted for 30 min, it is clear that many densely dispersed nanoparticles with the size of $40 nm are homogeneously produced inside and outside the PDA film without aggregation (Fig.…”

Section: Characterizations Of Modified Pdms Microchipsmentioning

confidence: 99%

“…Then, a freshly prepared 0.1 M phosphate solution containing DA and HAuCl 4 (molar concentration ratio of 7.5:1) at pH 7.17 was filled into the microchannel and reacted for 30 min [29]. After the formation of PDA/Au NP coating, the solution in the channel was flushed out and the PDA/Au NP-modified microchannel was rinsed with water for 5 min to remove physical attachment of residuary DA on the channel surface.…”

Section: Fabrication and Modification Of Pdms Microchipsmentioning

confidence: 99%

“…Up to now, although the chemical composition is not precisely known, catechol and quinine function groups are believed present in the PDA coating. Furthermore, aliquotted DA with catechol groups has been shown to be prone to oxidation [27]; thus, the adherent PDA can also be used to synthesize metallic nanoparticles, and the nitrogencontaining groups in PDA backbones are beneficial for the anchoring and dispersion of these nanoparticles [28][29][30][31]. To our knowledge, the in situ chemically induce the growth of metallic nanoparticles to the PDA-coated PDMS channels remains unexplored to date, and this mechanical and chemical protocol may endow the modified PDMS with interesting properties.…”

Section: Introductionmentioning

confidence: 99%

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PDMS microchip coated with polydopamine/gold nanoparticles hybrid for efficient electrophoresis separation of amino acids

et al. 2011

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In this paper, a novel, simple, economical and environmentally friendly method based on in situ chemically induced synthesis strategy was designed and developed for the modification of a poly(dimethylsiloxane) (PDMS) microchip channel with polydopamine/gold nanoparticles (PDA/Au NPs) to create a hydrophilic and biofouling resistant surface. Dopamine as a reductant and a monomer, and HAuCl(4) as an oxidant to trigger dopamine polymerization and the source of metallic nanoparticles, were filled into the PDMS microchannel to yield in situ a well-distributed and robust PDA/Au NP coating. Au NPs were highly and uniformly dispersed in/on the PDA matrix with a narrow size distribution, as verified by scanning electron microscopy and UV-vis spectra. Compared with the native PDMS microchannel, the modified surfaces exhibited much better wettability, high stability and suppressed electroosmotic mobility, and less nonspecific adsorption towards biomolecules. The water contact angle and EOF of PDA/Au NP-coated PDMS microchip were measured to be 13° and 4.17×10(-4) cm(2)/V s, compared to those of 111° and 5.33×10(-4) cm(2)/V s from the native one, respectively. Fast and efficient separations of five amino acids such as arginine, proline, histidine, valine and threonine suggested greatly improved electrophoretic performance of the PDA/Au NP-functionalized PDMS microchips. This one-step procedure offers an effective approach for a biomimetic surface design on microfluidic chips, which is promising in high-throughput and complex biological analysis.

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“…[39] In enzymes catalysis field, dopamine can be utilized to fabricate new polymer-enzyme-metallic nanoparticle composite films with a high load and a high-activity of immobilized enzymes for biosensing of glucose and galactose. [40] Simultaneously, the dopamine can coat magnetic nanoparticles onto cell surfaces during the self-polymerization for facilitating the use of cells in biocatalysis ( Figures 6A and 6B). [41] Based on the same principle, a polydopamine intermediate was used in the fabrication of 3-layer organic film on silicon wafer by a multistep self-assembly process.…”

Section: Intermediates Are Comprised Of Mussel-inspired Underwater Admentioning

confidence: 99%

Recent Progress of Mussel‐Inspired Underwater Adhesives

Zhang

Song

et al. 2017

Chin. J. Chem.

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Underwater adhesion is greatly desired in tissue transplantation, medical treatment, ocean transportation, and so on. However, common commercial polymeric adhesives are rather weakened and easily destroyed in water environment. In nature, some marine organisms, such as mussels, barnacles, or tube worms, exhibiting excellent underwater adhesion up to robust bonding on the rock of sea floor, can give exciting solutions to address the problem. Among these marine organisms, mussels exhibit unique underwater adhesion via the foot proteins of byssus. It has been verified that the catechol groups from the side chain of the mussel foot proteins is the main contribution to the unique underwater adhesion. Hence, inspired by the mussels' underwater adhesion, many mussel-mimetic polymers with catechol as end chains or side chains have been developed in the past decades. Here, we review recent progress of mussel-inspired underwater adhesives polymers from their catechol-functional design to their potential applications in intermediates, anti-biofouling, self-healing of hydrogels, biological adhesives, and drug delivery. The review may provide basis and help for the development of the commercial underwater adhesives.

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One‐Pot Preparation of Polymer–Enzyme–Metallic Nanoparticle Composite Films for High‐Performance Biosensing of Glucose and Galactose

Cited by 139 publications

References 52 publications

Fusion of nacre, mussel, and lotus leaf: bio-inspired graphene composite paper with multifunctional integration

Fusion of nacre, mussel, and lotus leaf: bio-inspired graphene composite paper with multifunctional integration

PDMS microchip coated with polydopamine/gold nanoparticles hybrid for efficient electrophoresis separation of amino acids

Recent Progress of Mussel‐Inspired Underwater Adhesives

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