Titanium-based
substrates are widely used in orthopedic treatments
and hard tissue engineering. However, many of these titanium (Ti)
substrates fail to interact properly between the cell-to-implant interface,
which can lead to loosening and dislocation from the implant site.
As a result, scaffold implant-associated complications and the need
for multiple surgeries lead to an increased clinical burden. To address
these challenges, we engineered osteoconductive and osteoinductive
biosubstrates of chitosan (CS)-cross-linked polyaniline (PANI) nanonets
coated on titanium nanotubes (TiO2NTs) in an attempt to
mimic bone tissue’s major extracellular matrix. Inspired by
the architectural and tunable mechanical properties of such tissue,
the TiO2NTs-PANI@CS-based biofilm conferred strong anticorrosion,
the ability to nucleate hydroxyapatite nanoparticles, and excellent
biocompatibility with human bone marrow-derived mesenchymal stem cells
(hBM-MSCs). An in vitro study showed that the substrate-supported
cell activities induced greater cell proliferation and differentiation
compared to cell-TiO2NTs alone. Notably, the bone-related
genes (collagen-I, OPN, OCN, and RUNX 2) were highly expressed within
TiO2NTs-PANI@CS over a period of 14 days, indicating greater
bone cell differentiation. These findings demonstrate that the in
vitro functionality of the cells on the osteoinductive-like platform
of TiO2NTs-PANI@CS improves the efficiency for osteoblastic
cell regeneration and that the substrate potentially has utility in
bone tissue engineering applications.