Electrochemical molecularly bioimprinted siloxane biosensor on the basis of core/shell silver nanoparticles/EGFR exon 21 L858R point mutant gene/siloxane film for ultra-sensing of Gemcitabine as a lung cancer chemotherapy medication

Shoja, Yalda; Kermanpur, A.; Karimzadeh, F.; Ghodsi, Javad; Rafati, Amir Abbas; Adhami, Siavash

doi:10.1016/j.bios.2019.111611

Cited by 31 publications

(14 citation statements)

References 68 publications

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“…However, these methods require the functionalization of the electrode surface with molecularly imprinted polymers (MIPs), which can be difficult to obtain and can also pose reproducibility problems. Other works included the use of electrodes modified with MIPs that quantified GMB by measuring its interaction with DNA strands [ 19 , 20 ]. The modified electrodes are also difficult to obtain, and the use of DNA can pose stability issues.…”

Section: Resultsmentioning

confidence: 99%

“…Different types of strategies were reported for the detection of GMB, such as chromatographic methods [ 10 , 11 , 12 , 13 ], which are also employed by the European Pharmacopoeia 10th edition [ 14 ], as well as spectrophotometric [ 15 ] and electrochemical methods [ 16 , 17 , 18 , 19 , 20 , 21 ]. The latter represent a promising alternative, since they are simple, fast and require small quantities of samples.…”

Section: Introductionmentioning

confidence: 99%

“…The main disadvantage of this method is its need for high pH values and, therefore, the need for sample pH modification for detection. Indirect approaches included the use of GMB interactions with DNA [ 21 ] or the use of molecularly imprinted polymers (MIP) with or without DNA probes [ 18 , 19 , 20 ] for GMB electrochemical detection. Despite the very low limits of detection of some of these methods [ 18 ], the modification of the electrode surface with either DNA or MIP requires complex steps, and such surfaces can become unstable over time.…”

Section: Introductionmentioning

confidence: 99%

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Gemcitabine Direct Electrochemical Detection from Pharmaceutical Formulations Using a Boron-Doped Diamond Electrode

et al. 2021

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The development of fast and easy-to-use methods for gemcitabine detection is of great interest for pharmaceutical formulation control in both research laboratories and hospitals. In this study, we report a simple, fast and direct electrochemical method for gemcitabine detection using a boron-doped diamond electrode. The electrochemical oxidation of gemcitabine on a boron-doped diamond electrode was found to be irreversible in differential pulse voltammetry, and scan rate influence studies demonstrated that the process is diffusion-controlled. The influence of the pH and supporting electrolytes were also tested, and the optimized differential pulse voltammetry method was linear in the range of 2.5–50 μg/mL, with a detection limit of 0.85 μg/mL in phosphate-buffered saline (pH 7.4; 0.1 M). An amperometric method was also optimized for gemcitabine detection. The linear range of the method was 0.5–65 μg/mL in phosphate-buffered saline of pH 7.4 as well as pH 5.5, the limit of detection being 0.15 μg/mL. The optimized differential pulse voltammetry and amperometric detection strategies were successfully applied to pharmaceutical formulations, and the results were compared to those obtained by high-performance liquid chromatography and UV-Vis spectrophotometry with good correlations.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gemcitabine Direct Electrochemical Detection from Pharmaceutical Formulations Using a Boron-Doped Diamond Electrode

et al. 2021

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“…The DNA/GO/GCE sensor showed a detection limit of 7.6 nM and was tested for MTX determination in spiked urine and blood serum samples. The interactions of EGFR exon 21-point mutant gene with the anticancer drug Gemcitabine was recently evaluated using a DNA biosensor as reported in [82] and summarized in Table 3. Gemcitabine is one of the most important therapeutic agents of early and advanced stages of nonsmall cell lung cancer.…”

Section: Electrochemical Nucleic Acid-based Sensorsmentioning

confidence: 99%

“…Finally, among the different bioelectrochemical approaches, it is worth mentioning that organic bioelectronics [98][99][100][101][102] is receiving great attention for its potential application in real-time selective noninvasive detection of chemical biomarkers, including drugs, metabolites, neurotransmitters, proteins, and hormones, in a variety of body fluids. The interactions of EGFR exon 21-point mutant gene with the anticancer drug Gemcitabine was recently evaluated using a DNA biosensor as reported in [82] and summarized in Table 3. Gemcitabine is one of the most important therapeutic agents of early and advanced stages of non-small cell lung cancer.…”

Section: Other Bioelectrochemical Approachesmentioning

confidence: 99%

Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Tadini‐Buoninsegni

Palchetti

2020

Sensors

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Cancer is a multifactorial family of diseases that is still a leading cause of death worldwide. More than 100 different types of cancer affecting over 60 human organs are known. Chemotherapy plays a central role for treating cancer. The development of new anticancer drugs or new uses for existing drugs is an exciting and increasing research area. This is particularly important since drug resistance and side effects can limit the efficacy of the chemotherapy. Thus, there is a need for multiplexed, cost-effective, rapid, and novel screening methods that can help to elucidate the mechanism of the action of anticancer drugs and the identification of novel drug candidates. This review focuses on different label-free bioelectrochemical approaches, in particular, impedance-based methods, the solid supported membranes technique, and the DNA-based electrochemical sensor, that can be used to evaluate the effects of anticancer drugs on nucleic acids, membrane transporters, and living cells. Some relevant examples of anticancer drug interactions are presented which demonstrate the usefulness of such methods for the characterization of the mechanism of action of anticancer drugs that are targeted against various biomolecules.

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Electrochemical Methods Applied for Bioanalysis: Differential Pulse Voltammetry and Square Wave Voltammetry

Machado

Cincotto

2021

Tools and Trends in Bioanalytical Chemistry

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Electrochemical molecularly bioimprinted siloxane biosensor on the basis of core/shell silver nanoparticles/EGFR exon 21 L858R point mutant gene/siloxane film for ultra-sensing of Gemcitabine as a lung cancer chemotherapy medication

Cited by 31 publications

References 68 publications

Gemcitabine Direct Electrochemical Detection from Pharmaceutical Formulations Using a Boron-Doped Diamond Electrode

Gemcitabine Direct Electrochemical Detection from Pharmaceutical Formulations Using a Boron-Doped Diamond Electrode

Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Electrochemical Methods Applied for Bioanalysis: Differential Pulse Voltammetry and Square Wave Voltammetry

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