Electrochemical method for measuring C-reactive protein using crown ether-phosphate ester ionophores

Merritt, Carey M.; Winkelman, James W.

doi:10.1021/ac00196a009

Cited by 14 publications

(8 citation statements)

References 13 publications

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“…[45][46][47][48] Concentrations of CRP are reported to be elevated up to 1000-fold (200 µg/mL) following injury, inflammation, or infection. 48,49 Numerous recent studies have demonstrated that CRP can be used to help predict the risk of acute events in patients with atherosclerosis. 46,48,[50][51][52][53][54][55] CRP has also been shown to predict risk of future events in patients with acute coronary syndromes and in patients with stable angina and coronary artery stents.…”

mentioning

confidence: 99%

Electrogenerated Chemiluminescence. 72. Determination of Immobilized DNA and C-Reactive Protein on Au(111) Electrodes Using Tris(2,2‘-bipyridyl)ruthenium(II) Labels

2003

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Anodic electrogenerated chemiluminescence (ECL) with tri-n-propylamine (TPrA) as a coreactant was used to determine DNA and C-reactive protein (CRP) by immobilizations on Au(111) electrodes using tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)(3)(2+)) labels. A 23-mer synthetic single-stranded (ss) DNA derived from the Bacillus anthracis with an amino-modified group at the 5' end position was covalently attached to the Au(111) substrate precoated with a self-assembled thiol monolayer of 3-mercaptopropanoic acid (3-MPA) in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC) and then hybridized with a target ssDNA tagged with Ru(bpy)(3)(2+) ECL labels. Similarly, biotinylated anti-CRP species were immobilized effectively onto the Au(111) substrate precovered with a layer of avidin linked covalently via the reaction between avidin and a mixed thiol monolayer of 3-MPA and 16-mercaptohexadecanoic acid on Au(111) in the presence of EDAC and N-hydroxysuccinimide. CRP and anti-CRP tagged with Ru(bpy)(3)(2+) labels were then conjugated to the surface layer. ECL responses were generated from the modified electrodes described above by immersing them in a TPrA-containing electrolyte solution. A series of electrode treatments, including blocking free -COOH groups with ethanol amine, pinhole blocking with bovine serum albumin, washing with EDTA/NaCl/Tris buffer, and spraying with inert gases, were used to reduce the nonspecific adsorption of the labeled species. The ECL peak intensity was linearly proportional to the analyte CRP concentration over the range 1-24 microg/mL. CRP concentrations of two unknown human plasma/serum specimens were measured by the standard addition method based on this technique.

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confidence: 99%

Electrogenerated Chemiluminescence. 72. Determination of Immobilized DNA and C-Reactive Protein on Au(111) Electrodes Using Tris(2,2‘-bipyridyl)ruthenium(II) Labels

2003

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“…Again the detection of the bound CRP was carried out using CRP specific antibody. Merit and Winkelman have reported an electrochemical sensor for CRP based on crown ether [13]. Apart from this report, effort to develop synthetic entities to detect CRP, as far as we know, is not reported.…”

Section: Introductionmentioning

confidence: 86%

In vitro binding of C-reactive protein on to chemically modified polymethyl methacrylate surfaces

Sreenivasan

Vidya

Hari

2007

Sensors and Actuators B: Chemical

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“…Merritt and Winkelman have reported an electrochemical sensor for CRP based on crown ether [22]. Apart from these report chemical sensors for the detection of CRP have not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Nonconjugated Polyelectrolyte as Efficient Fluorescence Quencher and Their Applications as Biosensors: Polymer-Polymer Interaction

Vidya

Kunnetheeri

2014

ISRN Analytical Chemistry

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A simple fluorescence quenching method for the quantitation in serum of an acute phase reactant, C-reactive protein (CRP), which can differentiate between viral and bacterial infections, is described, where material and reagent costs are minimal. The study harnesses a fluorescence quenching between a nonfluorescent polyelectrolyte containing a ligand (O-phosphorylethanolamine, PEA) and fluorophore (fluorescamine Isomer 1) containing polyelectrolyte. The quenching was attributed due to strong polymerpolymer interaction through intermolecular hydrogen bonding. The nonlinear behaviour of Stern-Volmer plot indicates a binding induced quenching, that is, static quenching. However, fluorescence was found to increase in presence of C-reactive protein, due to the specific molecular recognition occurring between CRP and PEA, thereby excluding fluorophore containing chain. A definite correlation was found between concentration of CRP and fluorescence intensity and the method exhibited a linear relationship in the range of 40-360 ng/mL with a detection limit of 30 ± 2 ng/mL. The antibody free method was successfully applied for the analysis of CRP in human serum samples and the method showed good correlation with hospital measurements ( = 1.0313 −0.1423; = 32; = 0.9998, < 0.0001). Thus the fluorescence based polyelectrolyte biosensor is a potential system for rapid, and antibody free platform for CRP detection.

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Electrochemical method for measuring C-reactive protein using crown ether-phosphate ester ionophores

Cited by 14 publications

References 13 publications

Electrogenerated Chemiluminescence. 72. Determination of Immobilized DNA and C-Reactive Protein on Au(111) Electrodes Using Tris(2,2‘-bipyridyl)ruthenium(II) Labels

Electrogenerated Chemiluminescence. 72. Determination of Immobilized DNA and C-Reactive Protein on Au(111) Electrodes Using Tris(2,2‘-bipyridyl)ruthenium(II) Labels

In vitro binding of C-reactive protein on to chemically modified polymethyl methacrylate surfaces

Nonconjugated Polyelectrolyte as Efficient Fluorescence Quencher and Their Applications as Biosensors: Polymer-Polymer Interaction

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