In vitro binding of C-reactive protein on to chemically modified polymethyl methacrylate surfaces

“Functional upcycling” of polymer waste towards the design of new materials

“…Active functional groups can be generated via surface activation through hybrid methods, including air, 467 argon, 468 oxygen 450,451,454,[469][470][471][472] or water vapor plasma, 473 UV-irradiation [474][475][476][477][478][479] or ozone etching 452,480 (Fig. 16i and Table 7(1, 3, 5, 6)).…”

Self‐Assembled Polyamidoamine Dendrimer on Poly (methyl meth‐ acrylate) for Plasmonic Fiber Optic Sensors

“…In order to establish an optimal protocol for dendrimer immobilization, the decladded fiber probes were incubated in G4 PAMAM dendrimer solution of varying concentrations (5,15 and 50 μM) each for 4 h, 12 h and 24 h. The influence of different combination of dendrimer concentration and incubation time was evaluated by using EWA response due to FITC binding. The real-time absorbance response (at 494 nm) of these probes due to FITC binding is shown in Figure S3A, S3B and S3 C. Figure 3 shows the absorbance values obtained for all the nine probes coated for different time and concentration of dendrimer as a 3D matrix plot.…”

“…Despite a number of advantages, their chemical inertness is one of the major limitations in utilizing PMMA as a functional substrate for various biomedical applications. Till date, several chemical, physical or physicochemical methods have been investigated for PMMA surface modification in order to introduce functional groups such as amine, thiol, carbonyl and carboxylic groups . Most of these methods involve the pretreatment of PMMA surface with strong acids or alkali followed by conjugation of bifunctional agents.…”

“…Till date, several chemical, physical or physicochemical methods have been investigated for PMMA surface modification in order to introduce functional groups such as amine, thiol, carbonyl and carboxylic groups. [15][16][17][18][19][20] Most of these methods involve the pretreatment of PMMA surface with strong acids or alkali followed by conjugation of bifunctional agents. Several amine based homo bi-functional linkers including N-lithioethylene diamine, [12] ethylene diamine, [21] hexamethylene diamine (HMDA) [11] and 1,3-diaminopropane [18] or amine based polymeric agents such as polyethyleneimine (PEI) [22] and diamino polyethylene glycol (PEG) [23] have been reported to functionalize the PMMA substrates.…”