Electrochemical investigations of the interaction of C-reactive protein (CRP) with a CRP antibody chemically immobilized on a gold surface

Hennessey, Hooman; Afara, Nadia; Omanovic, Sasha; Padjen, Ante L.

doi:10.1016/j.aca.2009.04.009

Cited by 65 publications

(35 citation statements)

References 38 publications

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“…Also, the currents measured in CV and the resistances measured in EIS are inversely proportional to each other, qualitatively obeying the Ohm’s law. A distinctly measurable increase in ‘R ct ’ values after the surface modifications also indicates the high sensitivity of EIS compared to the CV technique, as reported by Hennessey et al (2009). Figure 3 (b) presents EIS curves for bare, Linker\anti-CRP and different CRP concentrations studied here.…”

Section: Resultssupporting

confidence: 74%

“…S3 (b) in the Supplementary Information shows the EIS of three electrodes (E1-E3). The EIS technique provides better sensitivity than CV by measuring the charge transfer resistance of exposed VANCFs interfaced with the solution (Hennessey et al, 2009). The complex plane (Nyquits plots) representation of impedance values in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…1 (b)) comprising of solution resistance component ‘R s (Ω)’ in series with time constant element comprising of charge transfer resistance component ‘R ct (Ω)’ and double layer capacitance C DL (F) component of REC. A better agreement between experimental and simulated curves can be obtained by using frequency-dependent constant-phase-elements (CPE) replacing pure ‘C DL ’ with ‘CPE DL ’ and in addition ‘CPE w ’ in series with ‘R ct ’ as suggested by Hennessey et al (2009). However, the ‘R ct ’ can easily be quantified from the predictable intersection of semicircle (experimental curve) on the horizontal axis (real impedance) in the EIS plot.…”

Section: Resultsmentioning

confidence: 99%

“…The reliable means of detection and quantification of CRP concentration thus becomes important for accurate implementation of therapeutic interventions before and after AMI occurrence for monitoring the status of AMI patients. Besides the traditional expensive and time-consuming methods of CRP testing (Roberts et al, 2000), quantification of CRP using surface plasmon resonance based biosensors (Hu et al, 2006), optical and acoustic biosensors (Luchansky et al, 2011; McBride et al, 2008), enzyme-linked immunosorbent assay (ELISA) (Leung et al, 2005), fluorescence based biosensors (Wolf et al, 2004), magnetic biosensors (Tsai et al, 2007; Martin et al, 2007), electrochemical biosensors based on gold electrode (Bryan et al 2013; Hennessey et al, 2009), magnetic nano-particles (Tsai et al, 2007) etc., has been demonstrated with a wide range of sensitivity, specificity, cost, turn around time (TAT), immunoassay complexity, flexibility for multiplexing, and detection limit (Qureshi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Label-free detection of C-reactive protein using a carbon nanofiber based biosensor

Gupta

Periyakaruppan

Meyyappan

et al. 2014

Biosensors and Bioelectronics

122

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We report the sensitive detection of C-reactive protein (CRP), a biomarker for cardiac disease, using a carbon nanofiber based biosensor platform. Vertically aligned carbon nanofibers were grown using plasma enhanced chemical vapor deposition to fabricate nanoelectrode arrays in a 3 X 3 configuration. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used for the CRP detection. The CV responses show a 25 % reduction in redox current upon the immobilization of anti-CRP on the electrode where as a 30% increase in charge transfer resistance is seen from EIS. Further reduction in redox current and increase in charge transfer resistance result from binding of CRP on anti-CRP immobilized surface, proportional to the concentration of the CRP target. The detection limit of the sensor is found to be ~90 pM or ~11 ng/ml, which is in the clinically relevant range. Control tests using non-specific myoglobin antigen confirmed the specificity of the present approach.

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Section: Resultssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Label-free detection of C-reactive protein using a carbon nanofiber based biosensor

Gupta

Periyakaruppan

Meyyappan

et al. 2014

Biosensors and Bioelectronics

122

View full text Add to dashboard Cite

show abstract

“…Sendo uma técnica produtiva, bem como mais refinada do ponto de vista do bem-estar animal, a tecnologia IgY tem sido aplicada com sucesso em fins científicos na saúde humana e veterinária, como em imunodiagnóstico, neutralização de toxinas de animais peçonhentos, imunoterapia, atividade bactericida e ensaios anti-parasitários (HORIE et al, 2004;CAI et al, 2012;MENDOZA et al, 2012;RAHMAN et al, 2013;SAMPAIO et al, 2014;SUNNWOO, 2015;GRANDO et al, 2017 (SILVA et al, 2009;HENNESSEY et al, 2009;BATRA et al, 2014). Baseando-se no elemento de reconhecimento biológico, o biossensores ao serem imobilizados com um anticorpo são classificados como imunossensores.…”

Section: -Imunoglobulina Yunclassified

Anticorpos IgY policlonais anti-fosfolipase A2: Ferramenta auxiliar para o estudo da ação anti-parasitária

Borges¹

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Activities of phospholipases (PLAs) have been linked to pathogenesis in various microorganisms. These are involved in processes of cell invasion, virulence, survival and remodeling / modification of the host cell phospholipid composition so the interest in these enzymes as potential targets that could contribute to the control of parasite survival and proliferation. Chicken eggs immunized with BnSP-7, a Lys49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, represent an excellent source of polyclonal antibodies with potential inhibitory activity on parasite PLAs. Herein, we report the production, characterization and anti-parasitic effect of IgY antibodies from egg yolks of hens immunized with BnSP-7. Produced antibodies presented increasing avidity and affinity for antigenic toxin epitopes throughout immunization, attaining a plateau after 4 weeks. Pooled egg yolks-purified anti-BnSP-7 IgY antibodies were able to specifically recognize different PLA2s from Bothrops pauloensis and Bothrops jararacussu venom. Antibodies also neutralized BnSP-7 cytotoxic activity in C2C12 cells. In addition, the antibodies recognized targets in Leishmania (Leishmania) amazonensis and Toxoplasma gondii extracts by ELISA and immunofluorescence assays. Anti-BnSP-7 IgY antibodies were cytotoxic to T. gondii tachyzoites and L. (L.) amazonensis promastigotes were able to decrease the proliferation of both parasites treated prior to infection and inhibit the invasion of promentigotes forms of L. (L.) amazonensis. Finally, anti-BnSP-7 antibodies were used to construct a nanocomposite modified graphite immunosensor, that could detect BnSP-7 and targets in L. (L.) amazonensis extract. These data suggest that anti-BnSP-7 IgY is an important tool for discovering new parasite targets, blocking parasitic effects and serving as a diagnostic tool.

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Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

et al. 2020

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This work describes the preparation of a reusable electrochemical immunosensor for detection of C-reactive protein (CRP), based in modified carbon electrode with reduced graphene oxide and polytyramine as strategy for anchoring the anti-CRP probe. This immunosensor was able to distinguish different concentrations of CRP target in human serum, by differential pulse voltammetry, showing a linear response from 1.09 to 100 μg L À 1. The performance of the immunosensor allowed its application in four consecutive assays, without activity loss. Analyses by atomic force microscopy, scanning electron microscopy and electrochemical impedance spectroscopy corroborate with voltammetric analysis. The immunosensor showed selectivity, stability and reusability, being a promising platform for point-of-care quantification of CRP.

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Electrochemical investigations of the interaction of C-reactive protein (CRP) with a CRP antibody chemically immobilized on a gold surface

Cited by 65 publications

References 38 publications

Label-free detection of C-reactive protein using a carbon nanofiber based biosensor

Label-free detection of C-reactive protein using a carbon nanofiber based biosensor

Anticorpos IgY policlonais anti-fosfolipase A2: Ferramenta auxiliar para o estudo da ação anti-parasitária

Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

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