“…The simplest and reliable technique for immobilization of azine type mediators is their adsorption at a suitable electrode like graphite. Redox dyes Nile Blue [5,6], Toluidine Blue [5,7,8], Meldola Blue [5,9,10], Coelestin Blue [11][12][13], Brilliant Cresyl Blue [5], and some others [14] have been immobilized by adsorption at graphite electrode, and used for electrocatalytic oxidation of NADH. It has been shown that a few tens of monolayers of the dye is usually adsorbed at a graphite surface [15].…”