Comparative investigation of NADH electrooxidation at graphite electrodes modified with two new phenothiazine derivatives

Lates, Vasilica; Gligor, Delia; Mureşan, Liana Maria; Popescu, Ionel Cătălin

doi:10.1016/j.jelechem.2011.07.046

Cited by 22 publications

(10 citation statements)

References 41 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was assumed that n = 2 (two electrons involved in the oxidation of NADH) and α was calculated to be 0.61. The electron transfer rate constant value calculated for ERGO-PAH/ SPE is the same order of magnitude with graphite electrode modified with phenothiazin derivates [30].. The performances of the modified electrode are highly dependent on the concentration of PAH solution and pH as it was previously reported [31].…”

Section: Cyclic Voltammetrysupporting

confidence: 70%

Electrochemical determination of NADH using screen printed carbon electrodes modified with reduced graphene oxide and poly(allylamine hydrochloride)

2015

View full text Add to dashboard Cite

We describe a new type of NADH sensor based on a screen-printed electrode (SPE) modified with reduced graphene oxide (RGO) and poly(allylamine hydrochloride) (PAH). A mixture of graphene oxide (GO) and PAH was deposited on the surface of a carbon working electrode, and RGO was prepared in-situ by electrochemical reduction of GO. The oxidation peak of NADH was recorded at +450 mV (vs. silver pseudo-reference). Under optimized conditions, the electrode exhibits high electrocatalytic activity toward NADH oxidation, expressed by a high rate constant and a stable response up to 0.8 mM concentrations. The sensitivity is 108.6 μA·mM −1 ·cm −2 , the response time is 20 s (for 95 % of the steady state current), and the detection limit is 6.6 μM (at an S/N ratio of 3). A peak separation of about 300 mV was achieved in differential pulse voltammetric determination of NADH in the presence of ascorbic acid. This makes the new sensor a useful tool with potential analytical application in different dehydrogenase based systems.

show abstract

Section: Cyclic Voltammetrysupporting

confidence: 70%

Electrochemical determination of NADH using screen printed carbon electrodes modified with reduced graphene oxide and poly(allylamine hydrochloride)

2015

View full text Add to dashboard Cite

show abstract

“…One strategy employed to overcome these difficulties (fouling and overvoltage and side reactions) was the use of mediator-modified electrodes, where the mediators are used to shuttle electrons from NADH to the electrode surface and allow electron transport between them [5,[9][10][11][12][13][14][15][16]. Some mediators (electrocatalysts) were immobilized on the electrode surface by covalent attachment, electrochemical polymerization, incorporation in carbon paste, adsorption, self-assembly and via entrapment in polymeric matrices [4,[17][18][19]. Another strategy is modification of the electrode surface with a polymeric substance, using electrodes modified with carbon nanotubes, nanofibers or using enzymatic methods that follow bioelectrocatalytic reaction [2,4,13,15,16,[20][21][22][23].…”

Section: David Publishingmentioning

confidence: 99%

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Ribau¹,

Fortunato²

2018

JPP

View full text Add to dashboard Cite

Abstract:A simple device which incorporates three electrodes (working electrode, counter electrode and reference electrode) was constructed to be used currently in laboratories without elevated cost. It does not need more than 2 µL of electrolyte, sample or working solution, his support material is paper, and the working electrode which is based on carbon ink can incorporate enzymes and cofactors. To test this concept we started this investigation using the NADH/NAD + redox couple which is an omnipresent coenzyme in living systems but is also a challenge to electrochemistry. The paper sensor fabrication was simple, rapid and cheaper. NADH was incorporated in the carbon ink by mixing both and, this mixture was used to print the working electrode. The direct electrochemical system NADH/NAD + signal obtained, using this device, appeared at low potentials. A quasi-reversible diffusional redox process was achieved and regeneration of the NADH after oxidation was reached. This small paper device was not only used to study the redox process of NAD + /NADH, but also its behavior in the presence of electroactive (ascorbic acid) and non-eletroactive species (glucose).

show abstract

“…Therefore, there exist two kinds of catalytic model for the electrooxidation of NADH: one is a direct electro-catalysis 13,15,16,36 and the other is called a redox mediator electro-catalysis. [25][26][27][28][29][30][31][32]37 Though these redox mediators are very efficient electron shuttles for NADH oxidation processes, hydrodynamic voltammetry on the mediator-modified electrode remains a challenge for examining the kinetic parameters of NADH electrooxidation due to possible mediator loss from the electrode surface.…”

Section: 10mentioning

confidence: 99%

Investigation of Direct Electrooxidation Behavior of NADH at a Chemically Modified Glassy Carbon Electrode

Jin

Peng

et al. 2015

J. Electrochem. Soc.

View full text Add to dashboard Cite

In this study, the composite material that made up polyglycine/3d-4f hybrid-metallic cyano-bridged mixed coordination polymers (CyMCP) was successfully decorated onto a glassy carbon rotating disk electrode (GC RDE) using electrodeposition. The voltammetric behavior of NADH showed typical direct electrooxidation characteristics on the modified GC electrode. Our polyglycine/CyMCP-modified GC RDE was able to measure the apparent heterogeneous electron transfer rate constant of NADH during its direct electrooxidation by preventing fouling effects on the working electrode, which would generally cause a decrease in the oxidative peak current of NADH. In addition, differential pulse voltammetry provided a large linear range between the oxidative peak current and the concentration of NADH from 2-1500 μM at the polyglycine/CyMCP/GC electrode. Finally, the apparent heterogeneous electron transfer rate constant (k s NADH ) of NADH was determined to be 1.73 × 10 −2 cm · s −1 using the polyglycine/CyMCP-modified GC RDE. Both reduced and oxidized forms of β-nicotinamide adenine dinucleotide (NADH and NAD + , respectively) are the coenzymes for a large number of dehydrogenase enzymes (>300) and components of biomarker systems.1 In fact, because of its very important role in the field of analytical electrochemistry, 2,3 bioelectrocatalysis 4-6 and biofuel cells, 7,8 the electrochemical investigation for the formal potential of NADH/NAD + redox couple has attracted much attention. 9,10Therefore, direct oxidation of NADH at untreated or unmodified classical solid electrodes is known to appear poor reversibility and occurs with considerable overpotential (e.g., 1.1 V on carbon and 1.3 V on platinum). [11][12][13][14][15][16] Furthermore, accumulation of oxidation products involving radical intermediates, such as dimer from the one-electron oxidation product NAD +. , 17,18 can cause deactivation and fouling of the electrode surface. Such contamination of the working electrode can largely lower the sensitivity and reproducibility of voltammetric measurements. Consequently, considerable effort has been devoted with a strategy of surface modification on the substrate of conventional materials electrode to attain the electrode's long term stability for determination of NADH at a lower potential. 2,[19][20][21] Over the last few decades, various carbon nanomaterial 22-24 and redox-active mediator materials [25][26][27][28][29][30][31][32][33][34][35] have been modified onto the electrode surface in order to alleviate and/or prevent higher overpotential and fouling problems of the electrode. Therefore, there exist two kinds of catalytic model for the electrooxidation of NADH: one is a direct electro-catalysis 13,15,16,36 and the other is called a redox mediator electro-catalysis. [25][26][27][28][29][30][31][32]37 Though these redox mediators are very efficient electron shuttles for NADH oxidation processes, hydrodynamic voltammetry on the mediator-modified electrode remains a challenge for examining the kinetic parameters of NADH elect...

show abstract

Comparative investigation of NADH electrooxidation at graphite electrodes modified with two new phenothiazine derivatives

Cited by 22 publications

References 41 publications

Electrochemical determination of NADH using screen printed carbon electrodes modified with reduced graphene oxide and poly(allylamine hydrochloride)

Electrochemical determination of NADH using screen printed carbon electrodes modified with reduced graphene oxide and poly(allylamine hydrochloride)

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Investigation of Direct Electrooxidation Behavior of NADH at a Chemically Modified Glassy Carbon Electrode

Contact Info

Product

Resources

About