Electrochemical investigation of cellobiose dehydrogenase from new fungal sources on Au electrodes

Stoica, Leonard; Dimcheva, Nina; Haltrich, Dietmar; Ruzgas, Tautgirdas; Gorton, Lo

doi:10.1016/j.bios.2004.09.018

Cited by 51 publications

(92 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This model is named electron-sink model and up-hill transfer of electrons from H r to F o /F r . was proven to be possible at low pH (3.5 -4.0) [25]. A detailed rapid kinetics study of the inter-domain electron transfer of P. chrysosporium CDH and its pH dependence is given by Igarashi et al [26].…”

Section: Introductionmentioning

confidence: 99%

“…The potential was varied from À 250 mV to þ 500 mV (vs. Ag j AgCl in 0.1 M KCl) in steps of 50 mVand the catalytic current was measured by injecting a volume of 50 mL from a 5 mM lactose solution in 50 mM citrate buffer, pH 5.5. The choice of a slightly acidic pH was made considering previous characterization of CDH from other fungi [21,25]. The results are illustrated in Figure 2.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Investigation of Graphite Electrodes Modified with Cellobiose Dehydrogenase from the Ascomycete Myriococcum thermophilum

et al. 2007

Self Cite

View full text Add to dashboard Cite

The catalytic properties of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on a graphite electrode were investigated for a large variety of carbohydrate substrates. The effects of applied potential, pH and buffer composition were tested and optimized, and the most suitable conditions were used to evaluate the detection limit, linear range, and sensitivity of the sensor for different carbohydrates in the flow injection mode. Subsequently, the long term stability of the modified electrodes was determined. Additionally, the direct and mediated electron transfer between the active site of the enzyme and the electrode has been investigated by amperometric flow injection measurements in the absence and presence of the mediator 1,4-benzoquinone in the presence of cellobiose or lactose.

show abstract

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Investigation of Graphite Electrodes Modified with Cellobiose Dehydrogenase from the Ascomycete Myriococcum thermophilum

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…15) In the case of OH-Alk-SAM, no protonation or deprotonation occurs at pH 4 and the surface charge of the OH-Alk-SAM is neutral. 22) It is reported that [Ru(NH 3À ions) can act as catalytic reagents for electron transfer between the electrode and the dissolved ions, and that the electron transfer between the dissolved ions and the electrode with SAM was enhanced. 15) Because most of the U(VI) exists in the di-oxo-cationic form in the solution, the adsorbed U on COOH-Alk-SAM (negatively charged SAM) can act as a catalytic reagent for the electron transfer as in the case of the above ions.…”

Section: Discussionmentioning

confidence: 99%

Sorption of U(VI) on the 4-Mercaptopyridine Self-Assembled Monolayer

Nankawa

Ozaki

Francis

et al. 2008

J. Nucl. Sci. Technol.

View full text Add to dashboard Cite

The sorption of U(VI) on the 4-mercaptopyridine self-assembled monolayer (4-PyS-SAM) on Au(111) was studied by cyclic voltammetry. Cyclic voltammograms (CVs) of the 4-PyS-SAM working electrode were obtained by contact with 1 mM UO 2 (NO 3 ) 2 solution, 1 mM UO 2 (NO 3 ) 2 and 50 mM acetic acid solution, or 1 mM UO 2 (NO 3 ) 2 and 50 mM oxalic acid solution for 6 h at pH 4. The reduction current of U(VI) to U(V) was detected in the CV. The CV of the U(VI) associated 4-PyS-SAM after transport to U(VI)-free 0.1 M NaClO 4 solution showed that the reduction current was detected in the cases of UO 2 (NO 3 ) 2 and U(VI)-acetate, but not in the case of U(VI)-oxalate solution, indicating that U(VI) was adsorbed on the 4-PyS-SAM from the UO 2 (NO 3 ) 2 and U(VI)-acetate solutions, but not from U(VI)-oxalate solution. These results suggest that stability of U(VI)-4-PyS-SAM is not so high that U(VI)-4-PyS-SAM cannot be formed in the presence of 50 mM oxalate.

show abstract

“…The mechanism of electron communication between Myriococcum thermophilum CDH and electrode was investigated on SAM modified gold [8,9] and also when adsorbed onto graphite electrodes [10]. The heme domain is supposed to be responsible for the direct electron transfer (DET) communication with the electrode.…”

Section: Introductionmentioning

confidence: 99%

Improvement of direct bioelectrocatalysis by cellobiose dehydrogenase on screen printed graphite electrodes using polyaniline modification

Trashin¹,

Haltrich²,

Ludwig³

et al. 2009

Bioelectrochemistry

Self Cite

View full text Add to dashboard Cite

Electrochemical investigation of cellobiose dehydrogenase from new fungal sources on Au electrodes

Cited by 51 publications

References 23 publications

Investigation of Graphite Electrodes Modified with Cellobiose Dehydrogenase from the Ascomycete Myriococcum thermophilum

Investigation of Graphite Electrodes Modified with Cellobiose Dehydrogenase from the Ascomycete Myriococcum thermophilum

Sorption of U(VI) on the 4-Mercaptopyridine Self-Assembled Monolayer

Improvement of direct bioelectrocatalysis by cellobiose dehydrogenase on screen printed graphite electrodes using polyaniline modification

Contact Info

Product

Resources

About