Electrochemical impedance spectroscopy of blood for sensitive detection of blood hematocrit, sedimentation and dielectric properties

Zhbanov, A. I.; Yang, Sung

doi:10.1039/c7ay00714k

Cited by 40 publications

(32 citation statements)

References 85 publications

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“…The determination of the cell constant of the coplanar sensor is tricky since the electrical field distributes unevenly. In this study, we adopted an experimental method from reference [38] to determine cell constant of the coplanar sensor. Using this method, the geometry variance due to operation error occurred in the fabrication process can be eliminated as the capacitance is measured for each individual device.…”

Section: Resultsmentioning

confidence: 99%

“…Given the relative permittivity of air and DI water are known as 1.00059 and 78.8 (at 23.9°C), by measuring the frequency‐dependent capacitance of empty channel and DI water‐filled channel, stray capacitance and the cell constant of the sensor were calculated, following the method reported in reference [38]. Specifically, the complex impedance of air was fitted to a single capacitor and the complex impedance of DI water was fitted to the equivalent circuit indicated in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…(A1–A2) Real and imaginary parts of the impedance of PBS, nRBCs suspended in PBS, 0.2 S/m medium, and nRBCs suspended in 0.2 S/m medium. (B) Comparison of the calculated cell dielectric properties (red and blue star symbols) of the nRBCs suspended in PBS or 0.2 S/m medium with the published values [13,14,38,43–51] (box plots). (C) Comparison of the conductivity of PBS at different dilutions measured by the devised sensor (solid lines) to that measured by the conductivity meter (dashed lines).…”

Section: Resultsmentioning

confidence: 99%

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Dielectric spectroscopy of red blood cells in sickle cell disease

Liu

Qiang

2021

Electrophoresis

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Hypoxia‐induced polymerization of sickle hemoglobin and the related ion diffusion across cell membrane can lead to changes in cell dielectric properties, which can potentially serve as label‐free, diagnostic biomarkers for sickle cell disease. This article presents a microfluidic‐based approach with on‐chip gas control for the impedance spectroscopy of suspended cells within the frequency range of 40 Hz to 110 MHz. A comprehensive bioimpedance of sickle cells under both normoxia and hypoxia is achieved rapidly (within ∼7 min) and is appropriated by small sample volumes (∼2.5 μL). Analysis of the sensing modeling is performed to obtain optimum conditions for dielectric spectroscopy of sickle cell suspensions and for extraction of single cell properties from the measured impedance spectra. The results of sickle cells show that upon hypoxia treatment, cell interior permittivity and conductivity increase, while cell membrane capacitance decreases. Moreover, the relative changes in cell dielectric parameters are found to be dependent on the sickle and fetal hemoglobin levels. In contrast, the changes in normal red blood cells between the hypoxia and normoxia states are unnoticeable. The results of sickle cells may serve as a reference to design dielectrophoresis‐based cell sorting and electrodeformation testing devices that require cell dielectric characteristics as input parameters. The demonstrated method for dielectric characterization of single cells from the impedance spectroscopy of cell suspensions can be potentially applied to other cell types and under varied gas conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Dielectric spectroscopy of red blood cells in sickle cell disease

Liu

Qiang

2021

Electrophoresis

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“…Besides the above methods, classical impedance measurement of blood using electrodes or probes was reported in [2][3][4] and more recently in [5,6]. The dielectric properties of blood can be extracted from the measured impedance data.…”

Section: Introductionmentioning

confidence: 99%

Quick, Single-Frequency Dielectric Characterization of Blood Samples of Pediatric Cancer Patients by a Cylindrical Capacitor: Pilot Study

Ghanbarzadeh-Daghian

Ahmadian

Ghanbarzadeh-Dagheyan

2020

Electronics

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In this paper, as an application in biometrics, the electrical capacitance of normal and cancerous blood samples is experimentally determined in order to test the null hypothesis that the electrical capacitance of the two samples differs. The samples taken from healthy donors and patients diagnosed with different types of hematologic cancer are examined by a cylindrical capacitor with blood as its dielectric. The capacitance of these samples is measured at room temperature and a single frequency of 120 Hz, well below the frequency where β -dispersion starts, using a simple LCR meter device. The measurements indicate that the capacitance of the blood increases under applied electric field for a short period of time and asymptotically reaches its steady-state value. The measured values for the healthy group agreed with previous data in the literature. By the use of the unpaired two-tailed T-test, it is found that cancerous blood has higher values of capacitance when compared to normal samples ( p < 0.05 ). The reasons that might lead to such alterations are discussed from a biological perspective. Moreover, based on correlation calculations, a strong negative association is observed between blood capacitance and red blood cell (RBC) count in each group. Furthermore, sensitivity (SE) and specificity (SP) analysis demonstrates that for a threshold value between 15 and 17 for the capacitance value, both SE and SP are 100%. These preliminary findings on capacitance values may pave the way for the development of inexpensive and easy-to-use diagnosis tools for hematologic cancers at medical facilities and for in-home use, especially for children.

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“…Analyses of these spectra have used equivalent circuit models that qualitatively relate physical parameters to phenomena occurring in electrochemical cells. The EIS of biological systems reveals changes in electrical properties with frequency (Zhbanov and Yang 2017 ). At 100 kHz and lower, signals are associated with lipid membranes, counter ion diffusion, cellular membrane potential, and displacement of charged ions surrounding charged membranes (Zhbanov and Yang 2017 ; Heilman et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

In-situ electrochemical analysis of microbial activity

et al. 2018

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Microbes have a wide range of metabolic capabilities available that makes them industrially useful organisms. Monitoring these metabolic processes is a crucial component in efficient industrial application. Unfortunately, monitoring these metabolic processes can often be invasive and time consuming and expensive, especially within an anaerobic environment. Electrochemical techniques, such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) offer a non-invasive approach to monitor microbial activity and growth. EIS and CV were used to monitor Clostridium phytofermentans, an anaerobic and endospore-forming bacterium. C. phytofermentans ferments a wide range of sugars into hydrogen, acetate, and ethanol as fermentation by-products. For this study, both traditional microbiological and electrochemical techniques were used to monitor the growth of C. phytofermentans and the formation of fermentation products. An irreversible reduction peak was observed using CV beginning at mid-logarithmic phase of growth. This peak was associated with C. phytofermentans and not the spent medium and was indicative of a decrease in carbon and energy sources to the cells. Additionally, EIS analysis during growth provided information related to increased charge transfer resistance of the culture also as a function of carbon and energy source depletion. Results demonstrate that CV and EIS are useful tools in the monitoring the physiological status of bioprocesses.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0692-2) contains supplementary material, which is available to authorized users.

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Electrochemical impedance spectroscopy of blood for sensitive detection of blood hematocrit, sedimentation and dielectric properties

Abstract: Electrochemical impedance spectroscopy (EIS) is a highly promising tool for the analysis of blood.

Cited by 40 publications

References 85 publications

Dielectric spectroscopy of red blood cells in sickle cell disease

Dielectric spectroscopy of red blood cells in sickle cell disease

Quick, Single-Frequency Dielectric Characterization of Blood Samples of Pediatric Cancer Patients by a Cylindrical Capacitor: Pilot Study

In-situ electrochemical analysis of microbial activity

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