Electrochemical Impedance Spectroscopy as a Tool for Monitoring Cell Differentiation from Floor Plate Progenitors to Midbrain Neurons in Real Time

Elghajiji, Aya; Wang, Xin; Weston, Stephen D.; Zeck, Günther; Hengerer, Bastian; Tosh, David; Rocha, Paulo R. F.

doi:10.1002/adbi.202100330

Cited by 7 publications

(2 citation statements)

References 45 publications

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“…Furthermore, because the impedance spectrum is obtained from the oscillation waveform in every cycle, it enables an almost continuous impedance measurement. Continuous measurement of the impedance spectrum can be used to monitor biological processes such as cell differentiation [26].…”

Section: Introductionmentioning

confidence: 99%

Short-Time Impedance Spectroscopy Using a Mode-Switching Nonsinusoidal Oscillator: Applicability to Biological Tissues and Continuous Measurement

Yamaguchi

Ogawa

Ueno

2021

Sensors

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Herein, we propose an impedance spectroscopy method using a mode-switching nonsinusoidal oscillator and apply this method for measuring the impedance of biological tissues and continuous impedance measurement. To obtain impedance spectra over a wide frequency range, we fabricated a novel nonsinusoidal oscillator incorporating binary counters and analog switches. This oscillator could periodically switch oscillation frequency through the mode switching of the feedback resistor. From the oscillation waveform at each oscillation frequency of this circuit (oscillator), we determined the impedance spectrum of a measured object using the discrete-time Fourier transform. Subsequently, we obtained the broad impedance spectrum of the measured object by merging odd-order harmonic spectral components up to the 19th order for each oscillation frequency. From the measured spectrum, the resistive and capacitive components of the circuit simulating bioimpedance were estimated with high accuracy. Moreover, the proposed method was used to measure the impedance of porcine myocardium; changes in the impedance spectrum of the myocardial tissue due to coagulation could be measured. Furthermore, rapid variations in the resistance value of a CdS photocell could be continuously measured using the proposed method.

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Section: Introductionmentioning

confidence: 99%

Short-Time Impedance Spectroscopy Using a Mode-Switching Nonsinusoidal Oscillator: Applicability to Biological Tissues and Continuous Measurement

Yamaguchi

Ogawa

Ueno

2021

Sensors

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“…All electrical measurements were recorded using the PalmSens4 Electrochemical Interface, and were run at ten frequencies. Ten frequencies were selected between 500 Hz and 1600 Hz, in accordance with frequency ranges of interest for biosensor applications [9], and specifically for CA19-9 [10]. Results were obtained in the form of impedance magnitude and phase, and were processed to obtain real and imaginary components of impedance and capacitance, respectively.…”

Section: Antigen Incubation and Testingmentioning

confidence: 99%

A Capacitive Biosensor for the Early Detection of Pancreatic Cancer Using Carbohydrate Antigen 19-9

Ebrahim

Perold

Engelbrecht

2022

The 9th International Electronic Conference on Sensors and Applications

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Real‐Time, Non‐Invasive Monitoring of Neuronal Differentiation Using Intein‐Enabled Fluorescence Signal Translocation in Genetically Encoded Stem Cell‐Based Biosensors

Lee,

Choi,

Kwon

et al. 2024

Adv Funct Materials

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Real‐time and non‐invasive monitoring of neuronal differentiation helps to increase understanding of neuronal development and develop stem cell therapies for neurodegenerative diseases. Conventional methods such as RT‐PCR, western blotting, and immunofluorescence (IF), lack single‐cell‐level resolution and require invasive procedures, fixation, and staining. These limitations hinder accurate monitoring progress of neural stem cell (NSC) differentiation and understanding its functions. Herein, a novel approach is reported to non‐invasively monitor neuronal differentiation in real‐time using cell‐based biosensors (CBBs) that detects hippocalcin, biomarker of neuronal differentiation. To construct the hippocalcin sensor proteins, two different hippocalcin bioreceptors are fused to each split‐intein, carrying split‐nuclear localization signal (NLS) peptides, respectively, and fluorescent protein is introduced as reporter. CBBs operated in the presence of hippocalcin to generate functional signal peptides, which promptly translocated the fluorescence signal to the nucleus. The NSC‐based biosensor shows fluorescence signal translocation only upon neuronal differentiation and not undifferentiated stem cells or glial cells. Furthermore, this approach allows monitoring of neural differentiation at earlier stages than detected using IF staining. It is believed that novel CBBs offer an alternative to current techniques by capturing the dynamics of differentiation progress at the single‐cell‐level and providing a tool to evaluate how NSCs efficiently differentiate into neurons.

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Electrochemical Impedance Spectroscopy as a Tool for Monitoring Cell Differentiation from Floor Plate Progenitors to Midbrain Neurons in Real Time

Cited by 7 publications

References 45 publications

Short-Time Impedance Spectroscopy Using a Mode-Switching Nonsinusoidal Oscillator: Applicability to Biological Tissues and Continuous Measurement

Short-Time Impedance Spectroscopy Using a Mode-Switching Nonsinusoidal Oscillator: Applicability to Biological Tissues and Continuous Measurement

A Capacitive Biosensor for the Early Detection of Pancreatic Cancer Using Carbohydrate Antigen 19-9

Real‐Time, Non‐Invasive Monitoring of Neuronal Differentiation Using Intein‐Enabled Fluorescence Signal Translocation in Genetically Encoded Stem Cell‐Based Biosensors

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