Electrochemical immunosensor using artificial enzyme-induced metallization for the ultra-sensitive detection of alpha fetoprotein

Liao, Xiaochen; Wang, Xiao; Li, Peipei; Chen, Siyu; Zhang, Mengmeng; Mei, Lisha; Qi, Yu; Hong, Chenglin

doi:10.1016/j.snb.2021.130258

Cited by 20 publications

(13 citation statements)

References 41 publications

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“…The diffraction peak of Au NPs (PDF#99-0056) appears at 78.51° 30, belonging to its (311) crystal plane. 30 It can be seen from Fig. 1(J) that the composite nanomaterials contain Cu, O, C and Au elements.…”

Section: Resultsmentioning

confidence: 95%

Preparation of an electrochemical immunosensor based on a Cu/Cu₂O-rGO@Au signal synergistic amplification strategy and efficient and sensitive detection of alpha-fetoprotein

et al. 2022

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Section: Resultsmentioning

confidence: 95%

Preparation of an electrochemical immunosensor based on a Cu/Cu₂O-rGO@Au signal synergistic amplification strategy and efficient and sensitive detection of alpha-fetoprotein

et al. 2022

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“…Dopamine (DA), carcinoembryonic antigen (CEA) and AFP have similar physical and chemical structures [ 1 ], while glycine (Gly) is a coexisting substance of AFP. In addition, bovine serum albumin (BSA) may interfere with the determination of AFP content.…”

Section: Resultsmentioning

confidence: 99%

“…Alpha fetoprotein (AFP) is a type of glycoprotein that is closely related to the occurrence of liver cancer and a variety of tumors, and can be used as a serum marker of liver cancer in the clinical setting [ 1 ]. When the concentration of AFP in the body exceeds 30 ng·mL −1 , the presence of a malignant tumor can be considered.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Alpha-Fetoprotein Based on Black Phosphorus Nanosheets Modification with Iron Ions

Chen

Lin

et al. 2022

Micromachines

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Black phosphorus nanosheets (BPNSs) were synthesized with liquid exfoliation combined with the ultrasonic method and loaded with Fe3+ by simply mixing. The morphology, structure and electrochemical properties of the synthesized Fe3+/BPNSs were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry (CV), etc. The load of Fe3+ can improve the electrochemical performance of BPNSs and enhance the sensitivity of the detection. Additionally, Fe3+/BPNSs display good biocompatibility. In this study, immunosensors based on Fe3+/BPNSs were constructed to detect alpha-fetoprotein (AFP). The detection is due to the specific binding between the AFP antigen and antibody on the surface of the immunosensors, which can reduce the current response of Fe3+/BPNSs. The immunosensors have a good linear relationship in the range of 0.005 ng·mL−1 to 50 ng·mL−1, and the detection limit is 1.2 pg·mL−1. The results show that surface modification with metal ions is a simple and effective way to improve the electrochemical properties of BPNSs, which will broaden the prospects for the future application of BPNSs in the electrochemical field.

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“…AFP is a very specific antigen for primary liver cancer. 1 Liver cancer occupies the second place in the cause of cancer deaths and is harmful to human life and health, and many patients are not detected in time. As one of the most commonly used and most reliable markers for the detection of primary liver cancer, AFP level has become the most important early detection indicator.…”

Section: Introductionmentioning

confidence: 99%

“…AFP is a very specific antigen for primary liver cancer . Liver cancer occupies the second place in the cause of cancer deaths and is harmful to human life and health, and many patients are not detected in time.…”

Section: Introductionmentioning

confidence: 99%

MnO₂ Nanosheet/Polydopamine Double-Quenching Ru(bpy)₃²⁺@TMU-3 Electrochemiluminescence for Ultrasensitive Immunosensing of Alpha-Fetoprotein

Lai

et al. 2022

ACS Appl. Nano Mater.

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In this work, we propose a novel double-quenching electrochemical luminescence (ECL) strategy; this strategy is based on the double-quenching effect of Ru(bpy)3 2+-functionalized three-dimensional (3D) metal–organic framework (MOF) by MnO2/PDA. An ECL immunosensor for ultrasensitive detection of alpha-fetoprotein (AFP) has been designed. TMU-3 with a rich mesoporous structure can be used to encapsulate Ru(bpy)3 2+, which can effectively reduce the leakage of Ru(bpy)3 2+. Moreover, the ultraviolet–visible (UV–vis) absorption spectrum of the lamellar MnO2/PDA nanomaterials with a nanometer scale of about 1 μm overlaps with the ECL emission spectrum of the porous bamboo leaf-like Ru(bpy)3 2+@TMU-3 with a nanometer scale of about 1 μm. Thus, an ECL resonance energy transfer (ECL-RET) mechanism between Ru(bpy)3 2+@TMU-3 (donor) and MnO2/PDA (recipient) is formed. In this way, an ECL immunosensor with a dual-quenching strategy for ECL signal is developed. In order to test the practicability of this method in clinical analysis, the detection target marker AFP content ranged from 0.01 pg/mL to 5 ng/mL and the detection limit was set to 10.7 fg/mL. In summary, this work demonstrates the detection and application of a signal-quenched ECL immunosensor and ECL immunoassay based on a luminescence-functionalized MOF system for biomarker detection. It indicates a great prospect in the analysis of other biomarkers and clinical analysis.

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Electrochemical immunosensor using artificial enzyme-induced metallization for the ultra-sensitive detection of alpha fetoprotein

Cited by 20 publications

References 41 publications

Preparation of an electrochemical immunosensor based on a Cu/Cu₂O-rGO@Au signal synergistic amplification strategy and efficient and sensitive detection of alpha-fetoprotein

Preparation of an electrochemical immunosensor based on a Cu/Cu₂O-rGO@Au signal synergistic amplification strategy and efficient and sensitive detection of alpha-fetoprotein

Electrochemical Detection of Alpha-Fetoprotein Based on Black Phosphorus Nanosheets Modification with Iron Ions

MnO₂ Nanosheet/Polydopamine Double-Quenching Ru(bpy)₃²⁺@TMU-3 Electrochemiluminescence for Ultrasensitive Immunosensing of Alpha-Fetoprotein

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Electrochemical immunosensor using artificial enzyme-induced metallization for the ultra-sensitive detection of alpha fetoprotein

Cited by 20 publications

References 41 publications

Preparation of an electrochemical immunosensor based on a Cu/Cu2O-rGO@Au signal synergistic amplification strategy and efficient and sensitive detection of alpha-fetoprotein

Preparation of an electrochemical immunosensor based on a Cu/Cu2O-rGO@Au signal synergistic amplification strategy and efficient and sensitive detection of alpha-fetoprotein

Electrochemical Detection of Alpha-Fetoprotein Based on Black Phosphorus Nanosheets Modification with Iron Ions

MnO2 Nanosheet/Polydopamine Double-Quenching Ru(bpy)32+@TMU-3 Electrochemiluminescence for Ultrasensitive Immunosensing of Alpha-Fetoprotein

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Preparation of an electrochemical immunosensor based on a Cu/Cu₂O-rGO@Au signal synergistic amplification strategy and efficient and sensitive detection of alpha-fetoprotein

Preparation of an electrochemical immunosensor based on a Cu/Cu₂O-rGO@Au signal synergistic amplification strategy and efficient and sensitive detection of alpha-fetoprotein

MnO₂ Nanosheet/Polydopamine Double-Quenching Ru(bpy)₃²⁺@TMU-3 Electrochemiluminescence for Ultrasensitive Immunosensing of Alpha-Fetoprotein