Electrochemical immunosensor for HBe antigen detection based on a signal amplification strategy: The co-catalysis of horseradish peroxidase and nanoporous gold

Zhang, Yachao; Gao, Yan; Zhang, Xueli; Wang, Huiming; Xia, Tian; Bian, Congcong; Liang, Shuang; Tang, Xiaozhi; Wang, Xia

doi:10.1016/j.snb.2018.12.157

Cited by 28 publications

(13 citation statements)

References 46 publications

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“…264,265 Nanoporous gold electrodes amplify the electrochemical response as well as encourage the covalent binding of probes to reduce baseline current signals. 266,267 This concept can be integrated into PCR-based amplification to reduce the number of reaction cycles. 267 The concentration of AuNPs is another critical parameter that must be optimized as a higher concentration increases not only the color intensity 241 but also the possibility of nanoparticle agglomeration.…”

Section: As Wellmentioning

confidence: 99%

Nanomaterials for virus sensing and tracking

Pirzada

Altıntaş

2022

Chem. Soc. Rev.

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Section: As Wellmentioning

confidence: 99%

Nanomaterials for virus sensing and tracking

Pirzada

Altıntaş

2022

Chem. Soc. Rev.

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“…Various types of voltammetric techniques in combination with nanostructured supported material were used to determine avian influenza virus (H5N1) in chicken serum on porous Au nanoparticles [ 157 ] with an LOD of 1 pM, Hepatitis B e antigen detection based on a signal amplification by co-catalysis of horseradish peroxidase and nanoporous gold with an LOD of 64 fg/mL in human serum [ 158 ], human enterovirus 71 with dual-labeled magnetic nanobeads [ 159 ] with an LOD of 10 pg/mL, and many others [ 150 ]. The electrochemical immunosensor for corona virus associated with the Middle East respiratory syndrome (MERS) using an array of gold nanoparticle-modified carbon electrodes with voltammetric detection has been already designed [ 160 ].…”

Section: Detection Of Sars-cov-2mentioning

confidence: 99%

Wastewater-Based Epidemiology as an Early Warning System for the Spreading of SARS-CoV-2 and Its Mutations in the Population

Martı́n

Gál

Špalková

et al. 2021

IJERPH

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New methodologies based on the principle of “sewage epidemiology” have been successfully applied before in the detection of illegal drugs. The study describes the idea of early detection of a virus, e.g., SARS-CoV-2, in wastewater in order to focus on the area of virus occurrence and supplement the results obtained from clinical examination. By monitoring temporal variation in viral loads in wastewater in combination with other analysis, a virus outbreak can be detected and its spread can be suppressed early. The use of biosensors for virus detection also seems to be an interesting application. Biosensors are highly sensitive, selective, and portable and offer a way for fast analysis. This manuscript provides an overview of the current situation in the area of wastewater analysis, including genetic sequencing regarding viral detection and the technological solution of an early warning system for wastewater monitoring based on biosensors.

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“…NPG was fabricated according to our previous report 11 . A paper sensor with four CWE, one CCE, and one reference electrode (Ag/AgCl) was used as the multi-biocatalyst sensing chip.…”

Section: Preparation Of Cat-lipo/npg/cwe Bioelectrodementioning

confidence: 99%

“…The copyright holder for this preprint this version posted October 23, 2020. ; https://doi.org/10.1101/2020.10.22.348615 doi: bioRxiv preprint 5 min) 11 . Additionally, the use of specialized equipment, such as a microplate reader, limits the widespread application of ELISA-based detection in low-cost food industries.…”

Section: Introductionmentioning

confidence: 99%

A triple functional sensing chip for rapid detection of pathogenic Listeria monocytogenes

Zhang

Wang

Xiao

et al. 2020

Preprint

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Here a triple functional sensing chip was created for L. monocytogenes detection by integrating three biomarkers (Listeriolysin O (LLO) at protein level, hly gene at genetic level, and acetoin at metabolic level). Liposome encapsulated catechol was used for LLO detection via LLO pore-forming ability. hly gene was specifically captured by using a thiolated capture probe on nanoporous gold (NPG). As an electroactive label, methylene blue was embedded in double-stranded structures to generate an electrochemical signal for hly detection. Combined with the electrocatalysis of NADH by NPG, the acetoin detection was achieved by measuring the consumption of NADH as a cofactor under acetoin reductase catalysis. Importantly, the L. monocytogenes detection results obtained by detecting three biomarkers using the chip can be mutually verified, which reduces the probability of false positives based on a single marker. Moreover, the detection time was reduced to about 90 min, making it a rapid and reliable tool for L. monocytogenes detection.

show abstract

Electrochemical immunosensor for HBe antigen detection based on a signal amplification strategy: The co-catalysis of horseradish peroxidase and nanoporous gold

Cited by 28 publications

References 46 publications

Nanomaterials for virus sensing and tracking

Nanomaterials for virus sensing and tracking

Wastewater-Based Epidemiology as an Early Warning System for the Spreading of SARS-CoV-2 and Its Mutations in the Population

A triple functional sensing chip for rapid detection of pathogenic Listeria monocytogenes

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