Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C3N4/ZnO Nanocomposite-Modified Au Electrode

Kc, Saxena; Kumar, Arun; Chauhan, Nidhi; Khanuja, Manika; Malhotra, Bansi D.; Jain, Utkarsh

doi:10.1021/acsomega.2c03627

Cited by 18 publications

(11 citation statements)

References 59 publications

(82 reference statements)

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“…Our results are in line with many studies, (11) design an immunosensor able to detect a low concentration of VacA antigens in serum of H. pylori infected patients with high sensitivity and stability, suggesting to provide more early reliable clinical diagnosis for H. pylori infection.…”

Section: Discussionsupporting

confidence: 90%

Immunological Study of Gastroduodenal Disorders Patients Infected with H. Pylori in Basrah Province-Iraq

Hilfi¹,

Alsaimary²,

Hilfi³

2022

PJMHS

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The purpose of the current study was to investigate the immunological finding of the most important Helicobacter pylori virulence factors CagA, VacA, DupA, HopQ, and IceA genotypes in gastric biopsies of gastroduodenal diseases patients infected with H. pylori, and to determine the concentrations of CagA, VacA, DupA, HopQ, and IceA in the sera of the patients infected with H. pylori in Iraq-Basrah, by using ELISA technique, to give acknowledge about the role of these virulence factors in patients with the gastroduodenal disorder. A case-control study included 112 confirmed gastroduodenal patients and 112 healthy individuals as a control group. Data about age, gender, smoking, alcohol abuse, family history, occupation, residence, and clinical findings for all study populations were collected. Blood samples have been gathered from each patient as well as the control group. Each individual in this study was examined under the supervision of a gastroenterologist and was subjected to a urea breath test (UBT) for definitive confirmation of H. pylori infection. The finding of this study show a significant effect of these factors with an increased risk of gastroduodenal disorder. Conclusion: The study show a significant effect of the studied virulence factors (CagA, VacA, DupA, HopQ, and IceA) with an increased risk of gastroduodenal disorder of Iraqi population in Basrah province. Keywords: Immunological factors, gastroduodenal disorder, Helicobacter pylori, ELISA, CagA, VacA, DupA, HopQ, and IceA.

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Section: Discussionsupporting

confidence: 90%

Immunological Study of Gastroduodenal Disorders Patients Infected with H. Pylori in Basrah Province-Iraq

Hilfi¹,

Alsaimary²,

Hilfi³

2022

PJMHS

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“…All the measurements were taken in the presence of electrolytes containing 0.1 M K 3 [Fe(CN) 6 ] and K 4 [Fe(CN) 6 ] by using a three-electrode cell configuration. The study involved electrochemical measurement through cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

Fabrication of a Molecularly Imprinted Nano-Interface-Based Electrochemical Biosensor for the Detection of CagA Virulence Factors of H. pylori

Murti

Yang

et al. 2022

Biosensors

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H. pylori is responsible for several stomach-related diseases including gastric cancer. The main virulence factor responsible for its establishment in human gastric cells is known as CagA. Therefore, in this study, we have fabricated a highly sensitive MIP-based electrochemical biosensor for the detection of CagA. For this, an rGO and gold-coated, screen-printed electrode sensing platform was designed to provide a surface for the immobilization of a CagA-specific, molecularly imprinted polymer; then it was characterized electrochemically. Interestingly, molecular dynamics simulations were studied to optimize the MIP prepolymerization system, resulting in a well-matched, optimized molar ratio within the experiment. A low binding energy upon template removal indicates the capability of MIP to recognize the CagA antigen through a strong binding affinity. Under the optimized electrochemical experimental conditions, the fabricated CagA-MIP/Au/rGO@SPE sensor exhibited high sensitivity (0.275 µA ng−1 mL−1) and a very low limit of detection (0.05 ng mL−1) in a linear range of 0.05–50 ng mL−1. The influence of other possible interferents in analytical response has also been observed with the successful determination of the CagA antigen.

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“…The toxins were analyzed with a detection limit of 0.1 ng/mL for ricin and botulinum toxin A, of 0.05 ng/mL for staphylococcal enterotoxin B, and the time per single measurement was 15 min. A voltametric immunosensor was developed to detect vacuolating cytotoxin A from Helicobacter pylori [ 92 ]. Although this toxin is not listed among biological warfare agents, the assay provides promising results and can be easily adapted for other bacterial toxins.…”

Section: Progress On Immunosensors For Toxic Biological Warfare Agent...mentioning

confidence: 99%

Immunosensors for Assay of Toxic Biological Warfare Agents

Pohanka

2023

Biosensors

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An immunosensor for the assay of toxic biological warfare agents is a biosensor suitable for detecting hazardous substances such as aflatoxin, botulinum toxin, ricin, Shiga toxin, and others. The application of immunosensors is used in outdoor assays, point-of-care tests, as a spare method for more expensive devices, and even in the laboratory as a standard analytical method. Some immunosensors, such as automated flow-through analyzers or lateral flow tests, have been successfully commercialized as tools for toxins assay, but the research is ongoing. New devices are being developed, and the use of advanced materials and assay techniques make immunosensors highly competitive analytical devices in the field of toxic biological warfare agents assay. This review summarizes facts about current applications and new trends of immunosensors regarding recent papers in this area.

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Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C₃N₄/ZnO Nanocomposite-Modified Au Electrode

Cited by 18 publications

References 59 publications

Immunological Study of Gastroduodenal Disorders Patients Infected with H. Pylori in Basrah Province-Iraq

Immunological Study of Gastroduodenal Disorders Patients Infected with H. Pylori in Basrah Province-Iraq

Fabrication of a Molecularly Imprinted Nano-Interface-Based Electrochemical Biosensor for the Detection of CagA Virulence Factors of H. pylori

Immunosensors for Assay of Toxic Biological Warfare Agents

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