Electrochemical immunosensor based on hydrophilic polydopamine-coated prussian blue-mesoporous carbon for the rapid screening of 3-bromobiphenyl

Bifunctional magnetic nanobeads for sensitive detection of avian influenza A (H7N9) virus based on immunomagnetic separation and enzyme-induced metallization

“…On the one hand, MBs have been widely used as separation and enrichment carriers in pathogens detection (El-Boubbou et al, 2007;Chan et al, 2013), cell capture and separation (Song et al, 2011;Otieno et al, 2014), targeted drug delivery (Fuchigami et al, 2012;Anirudhan et al, 2013), magnetic imaging (Perez et al, 2004;Yu et al, 2013), and cancer therapy Santra et al, 2009). On the other hand, due to their large specific surface area and rich surface functional groups, MBs are also used as signal carriers to load a large amount of signal molecules to enhance the signal-to-noise ratio and improve the detection sensitivity (Mani et al, 2009;Shang et al, 2012), which is similar to the application of some nanoparticles, such as metal nanoparticles, carbon nanotubes, graphene, and polystyrene ball (Du et al, 2011;Li et al, 2013;Yan et al, 2012;Sun et al, 2014). Although MBs have been broadly used in biochemical analysis, the combined application of MBs both as separation and enrichment carriers and signal carriers has been rarely studied.…”

A multi-walled carbon nanotubes-poly(l-lysine) modified enantioselective immunosensor for ofloxacin by using multi-enzyme-labeled gold nanoflower as signal enhancer

“…Moreover, a significant investment in equipment should be considered. In the past decades, many immunoassay methods have been reported including chemiluminescence (Zangheri et al, 2015), fluorescence (Wu et al, 2015), quartz crystal microbalance (Ozalp et al, 2015), surface plasmon resonance (Ming et al, 2015), electrochemical immunoassays (Lin et al, 2012;Sun et al, 2014), and so on. Among these methods, electrochemical immunoassays, due to their intrinsic advantages such as good portability, low cost and high sensitivity, have been developed and extensively applied to the detection of various substance including tumor markers, food additives and environmental pollutants Scognamiglio et al, 2014).…”

Rapid Legionella pneumophila determination based on a disposable core–shell Fe 3 O 4 @poly(dopamine) magnetic nanoparticles immunoplatform

“…This polymer exhibits some interesting advantages over other coupling agent such as N-hydroxysuccinimide (NHS) or maleimide with respect to the lower susceptibility to hydrolysis which results in a higher conjugation efficiency [15]. Thus, recent works demonstrated the applicability of pDA-based immunosensors [16][17][18]. However, it is worth to mention that although MNPs@pDA have demonstrated previously their usefulness for the construction of electrochemical enzyme biosensors [13,14], their applicability to the preparation of electrochemical immunosensors have been explored only once [19].…”