Rapid Legionella pneumophila determination based on a disposable core–shell Fe 3 O 4 @poly(dopamine) magnetic nanoparticles immunoplatform

Martín, Miriam; Salazar, Pedro; Jiménez, Carmen; Lecuona, Marı́a; Ramos, Ma José; Ode, Jesús; Alcoba, Julia; Roche, R.; Villalonga, Reynaldo; Campuzano, Susana; Pingarrón, José M.; González–Mora, José Luis

doi:10.1016/j.aca.2015.05.048

Cited by 62 publications

(24 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, these nanoparticles (MNPs) are more prone to agglomerate and to suffer losses during their more challenging handling than their micrometric analogues [ 15 , 16 ]. In electrochemical biosensing approaches, MNPs have been used mainly as nanosurfaces where affinity conjugation takes place without the need for applying costly and complicated modification procedures to the electrode platforms [ 17 , 18 , 19 , 20 , 21 , 22 , 23 ], but also as catalytic label and as nanocarriers of signalling molecules.…”

Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

“…In the first type, the presence of gold nanoparticles provides good conductivity, improves the adsorption capacity of biomolecules and makes it possible to use thiolated derivatives to incorporate functionalities [ 20 ], while SiO 2 increases the stability of the nanoparticles providing a suitable surface for the binding of bioreactives [ 11 ]. Moreover, coatings of MNPs with polymers, such as polydopamine [ 17 ], allow increasing the immobilization capacity of biomolecules.…”

Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

Self Cite

View full text Add to dashboard Cite

The excellent capabilities demonstrated over the last few years by electrochemical affinity biosensors should be largely attributed to their coupling with particular nanostructures including dendrimers, DNA-based nanoskeletons, molecular imprinted polymers, metal-organic frameworks, nanozymes and magnetic and mesoporous silica nanoparticles. This review article aims to give, by highlighting representative methods reported in the last 5 years, an updated and general overview of the main improvements that the use of such well-ordered nanomaterials as electrode modifiers or advanced labels confer to electrochemical affinity biosensors in terms of sensitivity, selectivity, stability, conductivity and biocompatibility focused on food and environmental applications, less covered in the literature than clinics. A wide variety of bioreceptors (antibodies, DNAs, aptamers, lectins, mast cells, DNAzymes), affinity reactions (single, sandwich, competitive and displacement) and detection strategies (label-free or label-based using mainly natural but also artificial enzymes), whose performance is substantially improved when used in conjunction with nanostructured systems, are critically discussed together with the great diversity of molecular targets that nanostructured affinity biosensors are able to quantify using quite simple protocols in a wide variety of matrices and with the sensitivity required by legislation. The large number of possibilities and the versatility of these approaches, the main challenges to face in order to achieve other pursued capabilities (development of antifouling, continuous operation, wash-, calibration- and reagents-free devices, regulatory or Association of Official Analytical Chemists, AOAC, approval) and decisive future actions to achieve the commercialization and acceptance of these devices in our daily routine are also noted at the end.

show abstract

Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…One of the most effective and versatile strategies for biomolecules-nanoparticles coupling is the reaction via Schiff bases formation and/or Michael addition adducts of biomolecules amino groups and quinone groups on polydopamine modied surfaces. [37][38][39][40][41] Furthermore, the amount of antibodies conjugated on nanoparticle surfaces is critical for their recognition capability. In this case, the amount of AntiIgM (IgG isotype antibody) and IgM-dengue antibodies conjugated on core-shell Fe 3 O 4 @PDA nanoparticles and coated papers surfaces were determined by combining the reductive SDS-PAGE technique and Bradford assay (see ESI S6 for analysis details and Table S2 for data information †).…”

Section: Antibodies Conjugationmentioning

confidence: 99%

Magnetic paper – based ELISA for IgM-dengue detection

Ortega

Pérez-Rodriguez

Reguera³

2017

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…The LOD value achieved was 10 4 cfu·mL −1 with no need for pre-concentration or pre-enrichment treatments. This method was applied to the analysis of water samples [78]. Relevant aspects of this work are the good stability during 30 days of the Ab-Fe 3 O 4 @pDA NPs and the possibility of detecting bacteria at 10 CFU·mL −1 level in less than three hours after a preconcentration step.…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Yáñez‐Sedeño

Campuzano

Pingarrón

2016

Sensors

Self Cite

View full text Add to dashboard Cite

Ultrasensitive biosensing is currently a growing demand that has led to the development of numerous strategies for signal amplification. In this context, the unique properties of magnetic particles; both of nano- and micro-size dimensions; have proved to be promising materials to be coupled with disposable electrodes for the design of cost-effective electrochemical affinity biosensing platforms. This review addresses, through discussion of selected examples, the way that nano- and micro-magnetic particles (MNPs and MMPs; respectively) have contributed significantly to the development of electrochemical affinity biosensors, including immuno-, DNA, aptamer and other affinity modes. Different aspects such as type of magnetic particles, assay formats, detection techniques, sensitivity, applicability and other relevant characteristics are discussed. Research opportunities and future development trends in this field are also considered.

show abstract

Rapid Legionella pneumophila determination based on a disposable core–shell Fe 3 O 4 @poly(dopamine) magnetic nanoparticles immunoplatform

Cited by 62 publications

References 32 publications

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Magnetic paper – based ELISA for IgM-dengue detection

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Contact Info

Product

Resources

About