Electrochemical immobilization of ascorbate oxidase in poly(3,4‐ethylenedioxythiophene)/multiwalled carbon nanotubes composite films

Liu, Ming; Wen, Yang; Dong, Li Min; He, Haohua; Xu, Jingkun; Liu, Congcong; Yue, Ruirui; Lu, Baoyang; Liu, Guodong

doi:10.1002/app.34257

Cited by 22 publications

(12 citation statements)

References 54 publications

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“…Similar results have been reported for catalase covalently bound to electrospun nanofiber meshes filled with carbon nanotubes [40]. It was also reported that the conducting carbon nanotubes were able to promote direct electron transport from redox enzymes such as ascorbate oxidase, peroxidase, and Lac [38,[40][41][42][43][44][45][46]. Therefore, PpPD/Fe3O4 nanocomposite may also be suitable for enzyme immobilization, especially for redox enzymes.…”

Section: Removal Of Rb-19supporting

confidence: 80%

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

et al. 2016

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Magnetic poly(p-phenylenediamine) (PpPD) nanocomposite was synthesized via mixing p-phenylenediamine solution and Fe 3 O 4 nanoparticles and used as a carrier for immobilized enzymes. Successful synthesis of PpPD/Fe 3 O 4 nanofiber was confirmed by transmission electron microscopy and Fourier transform infrared spectroscopy. Laccase (Lac) was immobilized on the surface of PpPD/Fe 3 O 4 nanofiber through covalent bonding for reactive blue 19 dye removal. The immobilized Lac-nanofiber conjugates could be recovered from the reaction solution using a magnet. The optimum reaction pH and temperature for the immobilized Lac were 3.5 and 65 • C, respectively. The storage, operational stability, and thermal stability of the immobilized Lac were higher than those of its free counterpart. The dye removal efficiency of immobilized Lac was about 80% in the first 1 h of incubation, while that of free Lac was about 20%. It was found that the unique electronic properties of PpPD might underlie the high dye removal efficiency of immobilized Lac. Over a period of repeated operation, the dye removal efficiency was above 90% during the first two cycles and remained at about 43% after eight cycles. Immobilized Lac on PpPD/Fe 3 O 4 nanofiber showed high stability, easy recovery, reuse capabilities, and a high removal efficiency for reactive blue 19 dye; therefore, it provides an optional tool for dye removal from wastewater.

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Section: Removal Of Rb-19supporting

confidence: 80%

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

et al. 2016

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“…where R b is the R ct measured at a bare electrode, R m is the R ct measured at a modified electrode under same conditions [28,42,43]. The value of θ is about 65.64%, which also further confirmed that GOx were covalently immobilized onto the surface of ILs-PEDOTM-MWCNTs-COOH electrode.…”

Section: Electrochemical Impedance Spectroscopysupporting

confidence: 56%

Covalent Immobilization of Glucose Oxidase onto Electro-synthesized Nanocomposite with PEDOT Derivative for Amperometric Glucose Biosensing

Zhou¹

2018

International Journal of Electrochemical Science

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A highly sensitive and specific glucose (Glu) amperometric biosensor was successfully developed by the covalent immobilization of glucose oxidase (GOx) onto the electro-synthesized ionic liquid (ILs) modified poly(3,4-ethylenedioxylthiophene) derivative poly(hydroxymethylated-3,4ethylenedioxylthiophene) (PEDOTM) nanocomposite based on multi-walled carbon nanotubes functionalized with carboxyl group (MWCNTs-COOH). A highly-stable and conducting PEDOTM was one-step electro-synthesized in water/ILs mixed system containing BmimPF 6 and MWCNTs-COOH , then GOx was covalently immobilized onto the biocompatible ILs modified PEDOTM-MWCNTs-COOH with high affinity, which was employed for amperometric biosensing of Glu in human urine, human and animal serum samples. IL/PEDOTM-MWCNTs-COOH displayed good electrochemical activity, excellent electrochemical stability and high conductivity. The fabricated GOx biosensor showed pronounced amperometric current toward Glu response in a wide linear range of 6.0×10-8 ～2×10-3 M with a high sensitivity of 89.5 μA M −1 cm −2 , rapid response time within 10 s, low limit of detection of 0.015 μM, remarkable biocompatibility and bioaffinity, high sensing stability, excellent selectivity and practicality. Satisfactory results reveal that the ILs-PEDOTM-MWCNTs-COOH will provide a promising biosensing platform for the covalent immobilization of biomacromolecules and disease diagnostics via the detection of Glu in human and animal serum samples.

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“…The formation of this loop could be explained as the characteristics of nucleation process. As compared with EDOT polymerization, during electropolymerization process, the redox peaks of EDOT‐MeCl appeared at +0.46 V and +0.16 V, which attributed to the p ‐doping/dedoping processes of PEDOT‐MeCl film formed in previous scans. Upon sequential cycles, redox currents increased, implying that the formed electroactive and conductive layer on the gold electrode surface (light‐blue to blue‐black as the deposit thickened) was gradually increasing.…”

Section: Resultsmentioning

confidence: 94%

Electrosynthesis, characterization, and application of poly(3,4‐ethylenedioxythiophene) derivative with a chloromethyl functionality

Zhang

Wen

Yao

et al. 2013

J of Applied Polymer Sci

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Poly(2-chloromethyl-2,3-dihydrothieno [3,4-b][1,4]dioxine), a chloromethyl functionalized poly(3,4-ethylenedioxythiophene) derivative (PEDOT-MeCl), was synthesized electrochemically via the potentiostatic polymerization of its monomer in dichloromethane solution containing suitable tetrabutylammonium tetrafluoroborate, then it was used for the characterization of film properties and the fabrication of electrochemical sensor. The properties of the resulting PEDOT-MeCl film were characterized by different methods such as cyclic voltammetry, electrochemical impedance spectroscopy, Fourier transform infrared and ultraviolet-visible techniques, scanning electron microscope, and thermogravimetric analysis. The PEDOT-MeCl film displayed a good reversible redox activity, remarkable capacitance properties, good thermal stability, rough, and porous structure, especially fluorescent spectra indicated that PEDOT-MeCl was a blue-emitter with maximum emission centered at 396 and 398 nm. Finally, the PEDOT-MeCl film was employed for the fabrication of the sensing electrode, and dopamine was chosen as a model analyte for the application of the electrochemical sensor. Results indicated that the PEDOT-MeCl film as sensing interface was feasible, and studies of these film properties were very beneficial for studying properties and applications of other poly(3,4-ethylenedioxythiophene) derivative films.

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Electrochemical immobilization of ascorbate oxidase in poly(3,4‐ethylenedioxythiophene)/multiwalled carbon nanotubes composite films

Cited by 22 publications

References 54 publications

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

Covalent Immobilization of Glucose Oxidase onto Electro-synthesized Nanocomposite with PEDOT Derivative for Amperometric Glucose Biosensing

Electrosynthesis, characterization, and application of poly(3,4‐ethylenedioxythiophene) derivative with a chloromethyl functionality

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