Electrochemical Evaluation of Layer-by-Layer Drug Delivery Coating for Neural Interfaces

Abstract

Intracortical microelectrode arrays (MEAs) are valuable tools for neuroscience research, and their potential clinical use has been demonstrated. However, their inability to function reliably over chronic time points has limited their clinical translation. MEA failure is highly correlated with foreign body response (FBR), and therapeutics have been used to reduce FBR and improve device function, with drugs such as minocycline showing promising results in vivo. To avoid issues associated with systemic drug delivery, device coatings can be used to for therapeutic delivery. One method to locally deliver minocycline is a layer-by-layer (LBL) coating that consists of multiple trilayers of gelatin type A, minocycline, and dextran sulfate; however, the coating's impact on device function was previously unknown. This work characterized 10, 20, and 30 trilayer coatings and then evaluated their effect on device function. Cumulative minocycline release and coating thickness increased with the number of trilayers, agreeing with observations in previous studies. Atomic force microscopy images were used to calculate surface roughness of the coatings, which significantly increased from 10 to 20 trilayers and then remained relatively constant upon increasing to 30 trilayers. Scanning electron microscopy images confirmed that trilayers coated the MEAs. Electrochemical impedance spectroscopy (EIS) and charge carrying capacity (CCC) were used to evaluate the coating's effect on MEA electrochemical behavior over 3 weeks while the coated MEAs soaked in PBS. The 10 trilayer coatings slightly decreased CCC, while 20 and 30 trilayers initially increased CCC. CCC of all trilayers gradually increased as the MEAs soaked in PBS. All trilayers initially increased MEA impedance magnitude and reduced the phase angle at low frequencies. Impedance magnitude at 1 kHz and 15 kHz decreased toward their initial precoated values for all trilayers as the MEAs soaked in PBS. Overall, these results show that the LBL coatings did not significantly impact MEA function.

Engineering the Multienzymatic Activity of Cerium Oxide Nanoparticle Coatings for the Antioxidant Protection of Implants

“…CV technique was performed as previously described, with some modifications. [ 53 ] Briefly, a two centimeters stainless steel wires (127 μm diameter, A‐M Systems Inc., WA) were coated with different formulations of CONP and alginate. The coated stainless‐steel wire was submerged 1 cm into a MOPS buffer solution at room temperature and connected to an Autolab potentiostat PGSTAT12 (EcoChemie, Utrecht, The Netherlands) using a three‐electrode configuration.…”

Progress and challenges of implantable neural interfaces based on nature-derived materials

“…For these reasons, we believe that different NIs coatings made from nanostructure materials, such as layer-by-layer nanocoating and peptide functionalization, are preferable solutions to enhance biocompatibility for achieving better electrode tissue integration and electric performance (Olczak et al 2019 ).…”