Electrochemical dual-aptamer biosensors based on nanostructured multielectrode arrays for the detection of neuronal biomarkers

Zhang, Yuting; Figueroa-Miranda, Gabriela; Wu, Changtong; Willbold, Dieter; Offenhäusser, Andreas; Mayer, Dirk

doi:10.1039/d0nr03421e

Cited by 34 publications

(20 citation statements)

References 45 publications

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“…Construction of practical aptasensors for multiplex POCT may be the right step to make electrochemical aptasensors commercially attractive. There were a number of strategies reported for the multiplex analysis (Section 5) based on either simultaneous use of several different aptamers in an array format or of several redox-active probes encoding each aptamer/protein binding, and their further adaptation to multichannel, multianalyte [281], electrode array [282], and label-free detection schemes [283] may further assist the market activation of electrochemical aptasensors for cancer diagnosis. Therewith, the limitations to overcome also include the on-chip reagent storage and decreasing the cost of the miniaturised aptasensors.…”

Section: Discussionmentioning

confidence: 99%

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Malecka

Mikuła

Ferapontova

2021

Sensors

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Improved outcomes for many types of cancer achieved during recent years is due, among other factors, to the earlier detection of tumours and the greater availability of screening tests. With this, non-invasive, fast and accurate diagnostic devices for cancer diagnosis strongly improve the quality of healthcare by delivering screening results in the most cost-effective and safe way. Biosensors for cancer diagnostics exploiting aptamers offer several important advantages over traditional antibodies-based assays, such as the in-vitro aptamer production, their inexpensive and easy chemical synthesis and modification, and excellent thermal stability. On the other hand, electrochemical biosensing approaches allow sensitive, accurate and inexpensive way of sensing, due to the rapid detection with lower costs, smaller equipment size and lower power requirements. This review presents an up-to-date assessment of the recent design strategies and analytical performance of the electrochemical aptamer-based biosensors for cancer diagnosis and their future perspectives in cancer diagnostics.

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Section: Discussionmentioning

confidence: 99%

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Malecka

Mikuła

Ferapontova

2021

Sensors

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“…A dual-aptamer functionalized 3D nanostructure equipped with a multi-electrode array chip was designed to simultaneously quantify ATP and Aβ 40 oligomers ( Figure 3 D ). This dual-AD-biomarkers detecting biosensor provided a potential tool for accurate AD diagnosis strategies 112 .…”

Section: The Applications Of Aptamer In Aβ Detectionmentioning

confidence: 99%

Advances in aptamers against Aβ and applications in Aβ detection and regulation for Alzheimer's disease

Zheng¹,

Zhang²,

Zhao³

et al. 2022

Theranostics

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Alzheimer's disease (AD) is an irreversible neurodegenerative disease, causing profound social and economic implications. Early diagnosis and treatment of AD have faced great challenges due to the slow and hidden onset. β-amyloid (Aβ) protein has been considered an important biomarker and therapeutic target for AD. Therefore, non-invasive, simple, rapid and real-time detection methods for AD biomarkers are particularly favored. With the development of Aβ aptamers, the specific recognition between aptamers and Aβ plays a significant role in AD theranostics. On the one hand, aptamers are applied to construct biosensors for Aβ detection, which provides possibilities for early diagnosis of AD. On the other hand, aptamers are used for regulating Aβ aggregation process, which provides potential strategies for AD treatment. Many excellent reviews have summarized aptamers for neurodegenerative diseases or biosensors using specific recognition probes for Aβ detection applications in AD. In this review, we highlight the crucial role of the design, classification and applications of aptamers on Aβ detection as well as inhibition of Aβ aggregation for AD.

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“…[ 84 ] Zhang et al detected two small molecules using biosensors ( Figure 5 A), such as amyloid oligomer (AβO) and adenosine triphosphate (ATP). [ 85 ] They developed dual‐aptamer‐based multielectrode arrays for the simultaneous detection of AβO and ATP in artificial cerebrospinal fluid. The obtained dual‐target aptasensor exhibited linear detection for ATP ranging from 10 pM to 1000 nM with a LOD of 2 pM, and linear detection for AβO ranging from 1 pM to 200 nM with a low LOD of 0.3 pM.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Section: Biomedical Applicationsmentioning

confidence: 99%

Nanomaterial‐Based Electrochemical Sensors: Mechanism, Preparation, and Application in Biomedicine

Liu

Huang

Qian

2021

Advanced NanoBiomed Research

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Nanomaterials possess unique properties, including excellent electrochemical properties, high surface area, and tunable electric conductivity, making them attractive for sensing applications, especially for electrochemical sensing. Notably, the compatibility of nanomaterials with other techniques opens up opportunities for electrochemical sensors in various biomedical applications. Herein, the enhancing mechanisms and preparation protocols of electrochemical sensors, regarding the material choices, are introduced. The integration of nanomaterial‐based electrochemical sensors with other techniques toward precise health management and controlled drug release is further highlighted. It is concluded with perspectives on the future directions for this topic. Future research directions of nanomaterial‐based electrochemical sensors and the challenges faced by commercialized implementation of the sensors are presented, paving the way for the upcoming era of accurate biosensing toward both academic and industrial use.

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Electrochemical dual-aptamer biosensors based on nanostructured multielectrode arrays for the detection of neuronal biomarkers

Abstract:
Multielectrode arrays (MEAs) have been increasingly used for the development of biosensors due to their capability to record signals from multiple channels, fast mass transfer rates, and high spatial resolution....

Cited by 34 publications

References 45 publications

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Advances in aptamers against Aβ and applications in Aβ detection and regulation for Alzheimer's disease

Nanomaterial‐Based Electrochemical Sensors: Mechanism, Preparation, and Application in Biomedicine

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Electrochemical dual-aptamer biosensors based on nanostructured multielectrode arrays for the detection of neuronal biomarkers

Abstract: Multielectrode arrays (MEAs) have been increasingly used for the development of biosensors due to their capability to record signals from multiple channels, fast mass transfer rates, and high spatial resolution....

Cited by 34 publications

References 45 publications

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Advances in aptamers against Aβ and applications in Aβ detection and regulation for Alzheimer's disease

Nanomaterial‐Based Electrochemical Sensors: Mechanism, Preparation, and Application in Biomedicine

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Product

Resources

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Abstract:
Multielectrode arrays (MEAs) have been increasingly used for the development of biosensors due to their capability to record signals from multiple channels, fast mass transfer rates, and high spatial resolution....