Electrochemical DNA Sensor Based on Chitosan-Graphene and Electrodeposited Gold Nanoparticle Modified Electrode for the Detection of Staphylococcus aureus nuc Gene Sequence

Designing of a unique bioreceptor and fabrication of an efficient genosensing platform for neonatal sepsis detection

“…The developed sensor showed a linear detection range from 1 × 10 −13 mol L −1 to 1 × 10 −6 mol L −1 with an LOD of 3.3 × 10 −14 mol L −1 . 27 Moreover, an electrochemical genosensor was developed for E. coli detection using a GO–nickel ferrite–chitosan (GO/NiF/ch) nanocomposite film as the sensing platform. The fabricated biosensor exhibited a linear response to target complementary DNA in the concentration range of 10 −6 M to 10 −16 M with an LOD of 10 −16 M. 28 A study by Jaiswal et al showed the use of aminopropyltrimethoxysilane (APTMS)-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNF) in a composite that was electrophoretically deposited on an indium tin oxide (ITO)-coated glass substrate, which was further immobilized with an E. coli specific DNA probe.…”

Single-Layered Graphene/Au-Nanoparticles-Based Love Wave Biosensor for Highly Sensitive and Specific Detection of Staphylococcus aureus Gene Sequences

“…In addition, S. aureus can also cause staphylococcal food poisoning, which is regarded as one of the major foodborne illnesses in the world . Currently, common detection methods of S. aureus include enzyme-linked immunosensor assay, real-time polymerase chain reaction (PCR), metal–organic frameworks, magnetic particle enhanced surface plasmon spectroscopy, polydopamine-modified magnetic nanoparticles, and optical and electrochemical biosensors. − However, these methods have some limitations, such as high costs, requiring expensive precision instruments and professional operators, and long processing times, requiring labeled markers. Therefore, the demand for low costs and operationally simple, rapid, and label-free detection methods is urgently increasing.…”

A sensitive DNA biosensor based on the distance dependent quenching of Silica@Ru(bpy)32+‐chitosan‐GO electrochemiluminescence by Ferrocene@Gold nanoparticles

“…GO is a two-dimensional chemically modified graphene sheet. Its unique and excellent conductivity and thermodynamic properties have attracted extensive attention in different fields [24]. And it has excellent conductivity, biocompatibility, small size effect, and high surface chemical properties.…”