Designing of a unique bioreceptor and fabrication of an efficient genosensing platform for neonatal sepsis detection

Gopal, Neha; Chauhan, Nidhi; Jain, Utkarsh; Dass, Sujata K.; Kumar, Suveen; Chandra, Ramesh

doi:10.1039/d3ay00567d

Cited by 4 publications

(2 citation statements)

References 51 publications

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“…Here a novel 20-mer oligonucleotide has been designed for the diagnosis of the fimA gene of E. coli that is responsible for neonatal sepsis. This indirect approach will not only help in the diagnosis of neonatal sepsis but also helps the doctor in the selection of selective drug against microorganism for the curing of this disease [7]. Along with this, it has been reported by WHO (World Health Organization) and UNICEF (United Nations Children's Fund) that drinking water should be free from E.coli, because it may cause to development of a lot of mammalian gastrointestinal diseases [8].…”

mentioning

confidence: 99%

Biosensor: An Emerging Technological Tool for Microorganisms and Its Disease Diagnosis

Yadav,

Prasad,

Singh

2023

Indian J Microbiol

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confidence: 99%

Biosensor: An Emerging Technological Tool for Microorganisms and Its Disease Diagnosis

Yadav,

Prasad,

Singh

2023

Indian J Microbiol

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“…The functional effect of removing genes of interest (Table 6.6) was investigated using ex vivo whole blood and THP-1 cell infection models. In addition, the mutant fimA was included in mutant studies due to its role during the initial stages of urinary tract infection as well as inthe abdominal tract and neonatal sepsis [467][468][469].In whole blood infections, the cytokines IL-8, MIP3α and TNFα were investigated. Greater levels of cytokine responses were induced by LPS in whole blood than in the THP-1 cells (Figure 6.1 versus 6.2).…”

Section: Ex Vivo Whole Blood and Thp-1 Cell Cytokine Response To E Co...mentioning

confidence: 99%

Identifying Host and Bacterial Biomarkers to Predict Sepsis

Lewis

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Sepsis is a serious concern for healthcare programmes worldwide and is associated with 11 million deaths annually. E. coli accounts for 20% of bloodstream infections worldwide and is responsible for 17% of sepsis related mortality. Early diagnosis and treatment are critical and delays in initiating antimicrobial therapy are linked to mortality. Identifying biomarkers to predict patients that might succumb to sepsis is vital to aid in early diagnosis. It was hypothesised that E. coli bacteraemia isolates and isolates from different sources of bacteraemia would elicit a distinctive host response and be genetically unique. Blood culture positive isolates (n=165) were collected from the Hywel Dda University Health Board. Most of the isolates were assigned to the B2 and D phylogroups and belonged to either ST131 or ST73. Antimicrobial resistance in the collection was lower than national averages. Host models of infection were used to identify phenotypic responses of the bacterial collection. IL-8 and MIP3α were increased following stimulation by bacteraemia isolates compared to non-pathogenic strains. Greater IL-8 in whole blood was associated with a urinary and abdominal bacteraemia. Isolates that were resistant to human plasma elicited a higher IL-6, IL-8 and resistin response in whole blood compared to plasma sensitive isolates. Blood culture positive bacteraemia isolates had significantly more virulence factors than control isolates. Bacteraemia isolates expressed more P fimbriae genes. The S fimbrial adhesin genes were found to be significantly different between urinary and abdominal isolates. Abdominal isolates had significantly more sfaC (32%) while urinary isolates had more sfaX (22%). GWAS analysis revealed 6 potential gene targets based on bacterial phenotypes. These were ynbC, yhgE, ybjE, yejF, tufB and yohF. The results contained within this thesis describe new targets for predicting bacteraemia and sepsis and underline the importance of using host and pathogen as sources for biomarkers.

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Nanomaterial Modified Screen Printed Electrode Based Electrochemical Genosensor for Efficient Detection of Neonatal Sepsis

Gopal,

Chauhan,

Jain

et al. 2024

Indian J Microbiol

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Designing of a unique bioreceptor and fabrication of an efficient genosensing platform for neonatal sepsis detection

Abstract: We report results of studies related to fabrication of nanostructured graphene oxide (GO) based electrochemical genosensor for neonatal sepsis detection. Initially, we have selected fimA gene of E. coli for...

Cited by 4 publications

References 51 publications

Biosensor: An Emerging Technological Tool for Microorganisms and Its Disease Diagnosis

Biosensor: An Emerging Technological Tool for Microorganisms and Its Disease Diagnosis

Identifying Host and Bacterial Biomarkers to Predict Sepsis

Nanomaterial Modified Screen Printed Electrode Based Electrochemical Genosensor for Efficient Detection of Neonatal Sepsis

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