Electrochemical DNA biosensor for the detection of DNA hybridization with the amplification of Au nanoparticles and CdS nanoparticles

Du, Ping; Li, Hongxia; Mei, Zhenhua; Liu, Shufeng

doi:10.1016/j.bioelechem.2009.01.003

Cited by 109 publications

(50 citation statements)

References 44 publications

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“…Many kinds of nanomaterial, including metal [9], oxide [10], and semiconductor [11] nanoparticles, can be used to improve immobilization of biomolecules. Combining nanoparticles with biomolecules improves biosensor performance because of their large surface area.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical DNA biosensor based on gold nanorods for detecting hepatitis B virus

et al. 2014

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The purpose of this work was to fabricate an electrochemical DNA biosensor for detecting hepatitis B virus. Gold nanorods (GNRs), which are known for their conductivity, were used to increase surface area and consequently increase the immobilization of single-stranded DNA (ss-DNA) on the modified gold electrode. The GNRs were characterized via transmission electron microscopy. The morphology of the gold electrode before and after modification with GNRs was characterized by scanning electron microscopy. Atomic-force microscopy was used to evaluate the morphology of the GNR electrode surface before and after interaction with ss-DNA. Cyclic voltammetry was used to monitor DNA immobilization and hybridization, using [Co(phen)3](3+) as an electrochemical indicator. The target DNA sequences were quantified at a linear range from 1.0 × 10(-12) to 10.0 × 10(-6) mol L(-1), with a detection limit of 2.0 × 10(-12) mol L(-1) by 3σ. The biosensor had good specificity for distinguishing complementary DNA in the presence of non-complementary and mismatched DNA sequences.

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Section: Introductionmentioning

confidence: 99%

Electrochemical DNA biosensor based on gold nanorods for detecting hepatitis B virus

et al. 2014

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“…Colloidal gold nanoparticles and carboxyl group-functionalized CdS Nanoparticles (CdS NPs) were immobilized on the Au electrode surface to fabricate a novel electrochemical www.intechopen.com DNA biosensor (Du et al, 2009). Both GNPs and CdS NPs, well known to be good biocompatible and conductive materials, could provide larger surface area and sufficient amount of binding points for DNA immobilization.…”

Section: Horseradish Peroxidasementioning

confidence: 99%

Nanobiosensors for health care

Atta¹,

Galal²,

Ali³

2011

Biosensors for Health, Environment and Biosecurity

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“…Methylene blue (MB), HAuCl 4 •4H 2 O, HCl, and sodium citrate were obtained from Shanghai Chemical Reagent Co., Ltd (China). Cu(NO 3 ) 2 •3H 2 O, NH 3 •H 2 O, and anhydrous ethanol were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).…”

Section: Reagents and Apparatusmentioning

confidence: 99%

“…As a result, a variety of DNA biosensors based on different detection strategies have been developed, including optical, [1,2] electrochemical, [3,4] acoustic [5,6] and gravimetric techniques. [7,8] Among them, electrochemical DNA biosensors are particularly attractive due to its simplicity, low cost, high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a Sensitive Electrochemical Biosensor for Detection of DNA Hybridization Based on Gold Nanoparticles/CuO Nanospindles Modified Glassy Carbon Electrode

2012

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In this work, a sensitive electrochemical DNA biosensor for the detection of sequence-specific target DNA was reported. Firstly, CuO nanospindles (CuO NS) were immobilized on the surface of a glassy carbon electrode (GCE). Subsequently, gold nanoparticles (Au NPs) were introduced to the surface of CuO NS by the electrochemical deposition mode. Probe DNA with SH (HS-DNA) at the 5'-phosphate end was covalently immobilized on the surface of the Au NPs through Au-S bond. Scanning electron microscopy (SEM) was used to elucidate the morphology of the assembled film, and electrochemical impedance spectroscopy technique (EIS) was used to investigate the DNA sensor assembly process. Hybridization detection of DNA was performed with differential pulse voltammetry (DPV) and the methylene blue (MB) was hybridization indicator. Under the optimal conditions, the decline of reduction peak current of MB (ΔI) was linear with the logarithm of the concentration of complementary DNA from 1.0× 10 -13 to 1.0×10 -6 mol•L -1 with a detection limit of 3.5×10 -14 mol•L -1 (S/N＝3). In addition, this DNA biosensor has good selectivity, and even can distinguish single-mismatched target DNA.

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Electrochemical DNA biosensor for the detection of DNA hybridization with the amplification of Au nanoparticles and CdS nanoparticles

Cited by 109 publications

References 44 publications

Electrochemical DNA biosensor based on gold nanorods for detecting hepatitis B virus

Electrochemical DNA biosensor based on gold nanorods for detecting hepatitis B virus

Nanobiosensors for health care

Fabrication of a Sensitive Electrochemical Biosensor for Detection of DNA Hybridization Based on Gold Nanoparticles/CuO Nanospindles Modified Glassy Carbon Electrode

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