Electrochemical DNA biosensor based on chitosan/nano-V2O5/MWCNTs composite film modified carbon ionic liquid electrode and its application to the LAMP product of Yersinia enterocolitica gene sequence

Sun, Wei; Qin, Peng; Gao, Hongwei; Li, Guicun; Jiao, Kui

doi:10.1016/j.bios.2009.10.011

Cited by 149 publications

(56 citation statements)

References 45 publications

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“…26 In brief, samples were diluted with 5.0 mM Tris-HCl buffer solution (pH 7.4) and denatured by heating it in boiling water bath for 10 min, and then frozen in an ice bath for 2 min. Then the solution of the denatured LAMP products was dropped onto the modified surface of the chip for hybridization.…”

Section: Methodsmentioning

confidence: 99%

Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips

Zhang¹,

Ji²,

Cui

et al. 2012

Bulletin of the Korean Chemical Society

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Five-channel electrochemical chips were fabricated based on the Micro-electromechanical System (MEMS) technology and were used as platforms to develop DNA arrays. Different kinds of thiolated DNA strands, whose sequences were related to white spot syndrome virus (WSSV) gene, were separately immobilized onto different working electrodes to fabricate a combinatorial biosensor system. As a result, different kinds of target DNA could be analyzed on one chip via a simultaneous recognition process using potassium ferricyanide as an indicator. To perform quantitative target DNA detection, a limit of 70 nM (S/N=3) was found in the presence of 600 nM coexisting noncomplementary ssDNA. The real samples of loop-mediated isothermal amplification (LAMP) products were detected by the proposed method with satisfactory result, suggesting that the multichannel chips had the potential for a high effective microdevice to recognize specific gene sequence for pointof-care applications.

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Section: Methodsmentioning

confidence: 99%

Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips

Zhang¹,

Ji²,

Cui

et al. 2012

Bulletin of the Korean Chemical Society

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“…al. [50] . ILs are known for their good biocompatibility with biomolecules and enzymes and they improve their reusability and stability.…”

Section: Ionogels: a Diverse Materials For Sensing Platformsmentioning

confidence: 99%

Wearable electrochemical sensors for monitoring performance athletes

Fraser¹,

Curto²,

Coyle³

et al. 2011

SPIE Proceedings

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Nowadays, wearable sensors such as heart rate monitors and pedometers are in common use. The use of wearable systems such as these for personalized exercise regimes for health and rehabilitation is particularly interesting. In particular, the true potential of wearable chemical sensors, which for the real-time ambulatory monitoring of bodily fluids such as tears, sweat, urine and blood has not been realized. Here we present a brief introduction into the fields of ionogels and organic electrochemical transistors, and in particular, the concept of an OECT transistor incorporated into a sticking-plaster, along with a printable "ionogel" to provide a wearable biosensor platform.

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“…1,[3][4][5][6] However, it is time-consuming and the requirement for electrophoresis and UV detection reduces the suitability for field applications. Though the other candidate, electrochemical hybridization biosensor, could obtain the result of LAMP reaction automatically by detecting the product DNA, 7 it is cost and time-consuming to prepare DNA biosensor. To sum up the above contents, it's plain to see that regardless of their great success, the existing instruments for detecting the result of LAMP reaction have some limitations.…”

Section: Introductionmentioning

confidence: 99%

Detection of LAMP Products Using CNT Array Electrode

Zhang

Chen

et al. 2011

J Chinese Chemical Soc

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We described here a novel strategy for discriminating target gene by integrating loop-mediated isothermal amplification (LAMP) and differential pulse voltammetry (DPV). After a successful LAMP reaction, the anodic peak current (i pa ) of the free 2¢-deoxyguanosine 5¢-triphosphate (dGTP) decreased remarkably at a carbon nanotubes array working electrode, owing to the consumption of free dGTP as one of reactive substrates. Thus, the change of current response was used to characterize the result of LAMP reaction. And hence the presence of the target gene in template DNA could be discriminated easily. The malB gene extracted from Escherichia coli cells was tested as a model. After the reaction for 30 min, the LAMP mix was scanned directly. Then the information of the target gene in 0.8 picogram template DNA was obtained accurately. The result was in good accordance with that obtained with optical-based methods (gel electrophoresis and fluorescent dye). The new strategy has the advantages of being very simple to perform, rapid response, elimination of post-amplification processing, avoidance of auxiliary reagents and low cost (there was almost no cost for the detection step). Therefore, it was quite promising for use in miniaturized devices and in the development of point-of-care applications.

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Electrochemical DNA biosensor based on chitosan/nano-V2O5/MWCNTs composite film modified carbon ionic liquid electrode and its application to the LAMP product of Yersinia enterocolitica gene sequence

Cited by 149 publications

References 45 publications

Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips

Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips

Wearable electrochemical sensors for monitoring performance athletes

Detection of LAMP Products Using CNT Array Electrode

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